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Aspergillus oryzae bacterial strain for producing feed compound enzyme with high yield and application of aspergillus oryzae bacterial strain

A technology of compound enzyme for feed and Aspergillus oryzae, applied in the directions of application, enzyme, hydrolase, etc., can solve the problems of complex steps and low production efficiency, and achieve the effect of easy cultivation

Inactive Publication Date: 2017-08-08
ANIMAL HUSBANDRY CO LTD JIANGSU DAY KIND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the production of polypeptides needs to use microbial fermentation to produce protease, and then use protease to partially degrade the macromolecular proteins that are difficult to be digested and absorbed by animals to produce a variety of small molecular active polypeptides. The steps are relatively complicated and the production efficiency is low.

Method used

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  • Aspergillus oryzae bacterial strain for producing feed compound enzyme with high yield and application of aspergillus oryzae bacterial strain
  • Aspergillus oryzae bacterial strain for producing feed compound enzyme with high yield and application of aspergillus oryzae bacterial strain
  • Aspergillus oryzae bacterial strain for producing feed compound enzyme with high yield and application of aspergillus oryzae bacterial strain

Examples

Experimental program
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Effect test

Embodiment 1

[0048] 1. Preparation of high-yield serving compound enzyme protease and inulinase strain

[0049] (1) Sampling and sample processing:

[0050] Take 20 soil samples from places rich in protein and Jerusalem artichoke, such as slaughterhouses, meat sales markets, bean product processing factories, and Jerusalem artichoke plantations in Xuzhou City, Jiangsu Province. Weigh 5g of the solid soil sample, put it into a conical flask filled with glass beads and 100mL of sterilized saline, shake it at room temperature at a speed of 180r / min for 20-30min, and make a suspension for use.

[0051] (2) Enrichment culture:

[0052] Take 2-4mL of the treated soil sample turbid liquid, add it to a 250mL Erlenmeyer flask filled with 30-60mL enrichment medium 1, and place it on a constant temperature shaker at 35-40°C and a speed of 180-200r / min Culture in medium for 5-7 days; then measure 30-50mL of enriched culture medium into a centrifuge tube under aseptic operation, centrifuge at 4000-50...

Embodiment 2

[0089] Prepare the polypeptide-rich feed additive (being applicable to the situation that the secondary seed culture liquid of required fermentation is less) with triangular flask fermentation, comprises the following steps:

[0090] Step 1, strain activation

[0091] Scrape 1 ring of Aspergillus oryzae spores from the preserved Aspergillus oryzae DJ36 strain slant, inoculate it on the activated solid seed medium of the strain, and culture it statically in a biochemical incubator at 28-32°C for 3-5 days. After the species is activated and the spores are fully mature, the culture is terminated.

[0092] Step 2, prepare the monospore suspension of Aspergillus oryzae

[0093] Wash the spores cultivated in step 1 with 0.75% sterile saline containing 0.01% v / v Tween 80, put them into a conical flask filled with 50 mL of sterile saline and sterile glass beads, and shake fully for 20 -30min. After the spores are fully dispersed, the prepared spore content is 1.0×10 8 CFU / mL of sp...

Embodiment 3

[0105] Prepare the polypeptide-rich feed additive by fermenting in a fermenter (applicable to the situation that the secondary seed culture liquid of the required fermentation is more), the difference between its preparation steps and Example 2 is that the preparation of the compound enzyme by liquid fermentation in step 4 The specific operation is:

[0106] Put 8-10L of fermentation medium in a 15L fermenter, raise the temperature of the jacket of the fermenter to 95°C, then directly pass steam into the fermentation medium, sterilize at 121°C for 20-30min, and gradually lower the temperature to 28- 32°C, take 400-1000mL of the secondary seed culture solution obtained in step 2 and inoculate it in the fermenter by flame method, the pressure of the fermenter is 0.05MPa; the initial fermentation control conditions are: temperature 28-32°C, initial pH of the fermentation medium 5. 5-6.5, sterile air ventilation ratio 1:0.6-0.8 (V / V), stirring speed 260-300r / min, keep dissolved ox...

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Abstract

The invention discloses an aspergillus oryzae bacterial strain for producing feed compound enzyme with high yield and application of the aspergillus oryzae bacterial strain, and belongs to the technical field of biological fermentation. The bacterial strain for fermentation is specifically aspergillus oryzae DJ36, and is collected in the China General Microbiological Culture Collection Center with a collection number of CGMCC NO.13575. A polypeptide feed preparing process is implemented through a liquid fermentation technology in conjunction with an enzymatic hydrolysis technology, and specifically comprises the following steps: preparing a monospore bacterial suspension of the aspergillus oryzae; preparing a liquid bacterial strain; performing liquid fermentation with a triangular flask or a fermentation tank; performing enzymatic hydrolysis on impure protein with fermentation liquid; drying, smashing, packaging and the like. A functional small-molecular polypeptide is generated efficiently under the coaction of aspergillus oryzae fermentation and enzymatic hydrolysis at the later stage of fermentation, the advantages of a microbial fermentation technology and the enzymatic hydrolysis technology are combined, the use of an enzyme preparation is greatly reduced, the production cost of the polypeptide is lowered, and the industrialization process of the polypeptide is accelerated; moreover, a prepared feed additive has a relatively high nutrient value and disease resistance, and a wide application prospect.

Description

technical field [0001] The invention relates to the technical field of biological fermentation, in particular to a high-yield feed compound enzyme Aspergillus oryzae strain and application thereof. Background technique [0002] Agricultural and sideline products such as soybean meal, cottonseed meal, gluten powder, and feather meal have high crude protein content and low price, but the protein molecular weight is large, it is not easy to be digested and utilized, the amino acid composition is unbalanced, and the effective utilization rate is low. Utilization is low. At the same time, there are a variety of anti-nutritional factors, such as trypsin inhibitor, urease, etc. Studies have found that these anti-nutritional factors affect the digestion and absorption of nutrients by livestock and poultry, and affect the health and production performance of animals. During the fermentation process of agricultural and sideline products, various enzyme activities such as protease and...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12N1/02C12N9/24C12N9/62A23K10/12A23K20/147C12R1/69
CPCC12N1/02C12N1/14C12N9/2402C12N9/62C12Y302/01007C12N1/145C12R2001/69
Inventor 高兆建张铁柱唐仕荣李先双赵志刚康志锋冯奔
Owner ANIMAL HUSBANDRY CO LTD JIANGSU DAY KIND
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