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Method for preparing gamma-polyglutamic acid by utilizing gamma-polyglutamic acid producing bacteria

A technology for producing polyglutamic acid and bacteria, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of complex production conditions and difficult production operations, and achieve simple operation, low cost, and high production efficiency. high rate effect

Inactive Publication Date: 2017-08-08
广东华汗农业科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a method for preparing gamma-polyglutamic acid by a gamma-polyglutamic acid-producing bacterium, which solves the complex production conditions and production problems of gamma-polyglutamic acid prepared by Bacillus licheniformis in the prior art. Problems that are not easy to operate

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0027] A method for preparing gamma-polyglutamic acid by using gamma-polyglutamic acid producing bacteria comprises the following steps:

[0028] S1. Preparation of gamma-polyglutamic acid-producing strains;

[0029] S2. preparing fermentation medium;

[0030] S3. fermentation medium sterilization;

[0031] S4. Inoculating the seed solution obtained in S1 into a fermenter containing the fermentation medium obtained in S3 to form a culture solution;

[0032] S5. Fermenting the culture solution obtained in S4 in a shaker flask;

[0033] S6. adjusting the acidity of the culture medium after S5 shake flask fermentation, centrifuging, separating, and purifying with an organic solvent, and the purified supernatant is a γ-polyglutamic acid product;

[0034] Wherein, in the step S1, Bacillus licheniformis CICC10099 is used as the starting strain of the γ-polyglutamic acid producing bacterium seed liquid, and the seed liquid is prepared by seed fermentation culture, and the mother s...

Embodiment 2

[0042] Except that the raw material mass percentage of described preparation fermentation medium is different, other is with embodiment 1;

[0043] The fermentation medium contains the following raw materials in mass percentages: 0.8% glucose, 0.2% yeast powder, 0.06% dipotassium hydrogen phosphate, 9% L-glutamic acid, 0.05% magnesium sulfate, and the balance is water.

Embodiment 3

[0045] Except that the raw material mass percentage of described preparation fermentation medium is different, other is with embodiment 1;

[0046] The fermentation medium contains the following raw materials in mass percentages: 0.6% glucose, 0.12% yeast powder, 0.05% dipotassium hydrogen phosphate, 7% L-glutamic acid, 0.04% magnesium sulfate, and the balance is water.

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Abstract

The invention discloses a method for preparing gamma-polyglutamic acid by utilizing gamma-polyglutamic acid producing bacteria. The method comprises the following steps: the gamma-polyglutamic acid producing bacteria take bacillus licheniformis CICC10099 as a starting strain and preparing a seed liquid through the gamma-polyglutamic acid producing bacterium; preparing a fermentation culture medium; sterilizing the fermentation culture medium; inoculating strains and carrying out fermentation culture in a medicine bottle and the like to generate the gamma-polyglutamic acid; finally separating and purifying to obtain a gamma-polyglutamic acid product. According to the method disclosed by the invention, a process for producing the gamma-polyglutamic acid is simple and is convenient to operate; complicated fermentation tools and fermentation conditions are not needed; the concentration of the gamma-polyglutamic acid can reach 40g / l at maximum; a product has high purity and good quality and is suitable for large-area popularization in actual production.

Description

technical field [0001] The invention relates to the field of preparation and production of gamma-polyglutamic acid, in particular to a method for preparing gamma-polyglutamic acid by using gamma-polyglutamic acid producing bacteria. Background technique [0002] γ-polyglutamic acid (γ-PGA) is a kind of polyglutamic acid biosynthesized by microorganisms, which is formed by condensation of D-glutamic acid monomer or L-glutamic acid monomer with carboxyl and amino groups. γ-polyglutamic acid is a multifunctional biological product with great development value and prospects. In recent years, it has been widely used in industrial fields as thickener, moisturizer, drug carrier, etc. It is a water-soluble and biodegradable new biopolymer material that can be synthesized by microorganisms. In the process of producing oligoglutamic acid, the use of microbial fermentation to produce polyglutamic acid has a good prospect. The production of polyglutamic acid by microbial fermentation ...

Claims

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Application Information

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IPC IPC(8): C12P13/02C12R1/10
CPCC12P13/02
Inventor 杨天光
Owner 广东华汗农业科技有限公司