Conjugate vaccine and preparation method thereof
A vaccine and conjugation technology, applied in the field of vaccines, can solve problems such as the inability to effectively activate the complement system, the reduction of polysaccharide-protein binding efficiency, and the transient immune response.
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Embodiment 1
[0107] 1. Preparation of magnetic nanospheres
[0108] 1. Take 2.24g FeSO 4 -7H 2 O and 3.24 g FeCl 3 -6H 2 O dissolved in 10 mL and 15 mL of dddH 2 In O, mix well after dissolving, add 100mL of dddH filtered to remove oxygen 2 O;
[0109] 2. In N 2 Stir for 5 minutes under the protection of the solution, add 50mL1mol / L NaOH solution at one time, then adjust the pH of the solution to 9-10, increase the stirring speed to 200-250r / min, and continue stirring for 30min;
[0110] 3. Transfer the reaction vessel to a 65-70°C water bath and continue to 2 Stirring and aging under the protection of 30min;
[0111] 4. After the reaction, set the volume to 100mL, and observe the synthesis of magnetic particles under a microscope;
[0112] 5. Dissolve 400mgPLGA in 10mLEA solvent as the oil phase (O), add 3mL of the above magnetic particle solution as the inner water phase (W 1 ), using an ultrasonic cell breaker (120W, 60s) to carry out colostrum in an ice-water bath to prepare ...
Embodiment 2
[0158] The difference between this example and Example 1 is that the carrier protein of the conjugated vaccine is tetanus toxoid carrier protein and PspA.
[0159] The composition analysis results of the prepared conjugated vaccine are consistent with the results obtained in Example 1 within the range of theoretical error.
Embodiment 3
[0161] The difference between this example and Example 1 is that the carrier protein of the conjugated vaccine is rotavirus carrier protein and tetanus toxoid carrier protein.
[0162] The composition analysis results of the prepared conjugated vaccine are consistent with the results obtained in Example 1 within the range of theoretical error.
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