Selenium-enriched natural multifunctional leaf fertilizer and preparation method thereof

A multi-functional, foliar fertilizer technology, applied in the preparation of organic fertilizers, biochemical equipment and methods, and methods based on microorganisms, can solve the problems of selenium that is not easily absorbed and utilized by plants, low bioavailability, soil pollution, etc. , achieve good preventive effect, long-lasting effect, and reduce dosage

Active Publication Date: 2017-08-11
HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Selenate and selenite are highly toxic. If they are not applied properly, they will easily pollute the soil and even cause harm to the human body

Method used

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  • Selenium-enriched natural multifunctional leaf fertilizer and preparation method thereof
  • Selenium-enriched natural multifunctional leaf fertilizer and preparation method thereof
  • Selenium-enriched natural multifunctional leaf fertilizer and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] The preparation process of the foliar fertilizer of the present embodiment is as follows:

[0041] (1) Breeding of selenium-enriched strains

[0042]Nitrogen ion implantation mutagenesis was performed on Candida utilis, and after multiple mutagenesis screening, a yeast mutant strain C.utilis cas3 tolerant to high concentrations of sodium selenite was obtained;

[0043] Candida utilis C.utilis was cultured on a YEPD culture plate for 24-30 hours, picked a single colony into a 250ml Erlenmeyer flask containing 100ml of YEPD liquid medium, 160-180rpm, shaking culture for 24-30 hours, and the bacterial liquid was centrifuged and washed 3 times , make a single cell suspension, so that the cell concentration is 10 8 ~10 9 individual / mL. Take 0.1mL of the bacterial suspension and evenly spread it on a sterile petri dish, and place it on a clean bench to air dry to form a bacterial film. in N + Ion implantation was carried out in the implanter with an energy of 18KeV. Six...

Embodiment 2

[0068] The preparation process of the foliar fertilizer of the present embodiment is as follows:

[0069] (1) Breeding of selenium-enriched strains

[0070] The breeding process of the selenium-enriched bacterial strain of this embodiment is the same as that of embodiment 1.

[0071] (2) Preparation of seed culture solution

[0072] The above-mentioned Candida utilis C.utilis cas3 was inoculated on the slant medium and cultured at 28°C for 20 hours to obtain the slant strain. The composition of the slant medium was: glucose 18g / L, peptone 18g / L, yeast extract 8g / L, selenium Sodium acid 25mg / L, agar 15g / L.

[0073] The above slant strains were inoculated into 80 mL of seed culture medium, and cultured with shaking at 28° C., 170 r / min, for 20 h, to obtain seed culture liquid. The composition of the seed medium is: malt extract 15g / L, peptone 12g / L, yeast extract 8g / L, sodium selenite 50mg / L.

[0074] (3) Fermentation culture

[0075] The composition of the fermentation med...

Embodiment 3

[0094] The preparation process of the foliar fertilizer of the present embodiment is as follows:

[0095] (1) Breeding of selenium-enriched strains

[0096] The breeding process of the selenium-enriched bacterial strain of this embodiment is the same as that of embodiment 1.

[0097] (2) Preparation of seed culture solution:

[0098] The above-mentioned Candida utilis C.utilis cas3 was inoculated on the slant medium and cultured at 28°C for 24 hours to obtain the slant strain. The composition of the slant medium was: glucose 20g / L, peptone 20g / L, yeast extract 10g / L, selenium Sodium acid 30mg / L, agar 18g / L.

[0099] Inoculate the above-mentioned slant strains into 80 mL of seed culture medium, culture at 28° C., 180 r / min, and shake for 24 hours to obtain a seed culture solution. The composition of the seed medium is: malt extract 20g / L, peptone 15g / L, yeast extract 10g / L, sodium selenite 60mg / L.

[0100] (3) Fermentation culture:

[0101] The composition of the fermentat...

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Abstract

The invention discloses a selenium-enriched natural multifunctional leaf fertilizer and a preparation method thereof. The preparation method comprises the following steps: breeding selenium-enriched strains, preparing seed culture solution, fermenting and cultivating to obtain selenium-enriched yeast hydrolysate, preparing rhus chinensis extracting solution, preparing microelement solution, and preparing the selenium-enriched natural multifunctional leaf fertilizer. According to the preparation method, selenium-enriched yeast C.utilis cas3 is used for fermenting to generate the organic selenium efficiently, and the organic selenium can be rapidly absorbed and used by plants. On one hand, the pollution damage to the environment and human body caused by directly applying a common selenium fertilizer containing selenite is prevented, and on the other hand the absorption and utilization of the selenium for the plants are greatly improved, the selenium content of crops, such as vegetables and fruits, is improved. A selenium-enriched product is produced.

Description

technical field [0001] The invention relates to a foliar fertilizer, in particular to a selenium-enriched natural multifunctional foliar fertilizer and a preparation method thereof. Background technique [0002] Selenium is one of the trace elements necessary for the life activities of organisms. It has important functions such as nourishing and repairing the myocardium, removing free radicals in the body, delaying aging, and regulating the microcirculation system of the human body. At present, with the continuous improvement of people's living standards, people's pursuit of health is more urgent, and the demand for selenium-rich foods is also increasing. Biological selenium enrichment is an effective way to obtain organic selenium, and intake of selenium-rich fruits and vegetables is the most important way for the human body to obtain organic selenium. However, the content of selenium in natural fruits and vegetables is low, and most of them need to use selenium fertilizer...

Claims

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Application Information

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IPC IPC(8): C05G3/00C05F17/00C12N1/16C12P1/02C12R1/72
CPCC05C3/00C05F17/00C05G3/00C05G5/27C12N1/16C12P1/02C05C11/00C05D3/00C05D5/00C05D9/00C05D9/02C05F11/00C05F11/08Y02W30/40
Inventor 黄胜威吴丽芳王钰婷蔡冬清
Owner HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
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