A method for simultaneously stabilizing lipase and protease in compound enzyme laundry detergent

A compound enzyme laundry detergent and lipase technology, which is applied in the field of daily chemical industry, can solve the problems of unsuitability for liquid detergent, excess, large system, etc., and achieve the effects of prolonging shelf life, wide application range, and simple and easy to use products

Active Publication Date: 2019-10-15
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These methods can make protease and lipase have a certain stability in laundry detergent, but they are still not ideal: protease systems such as alginate or surface immobilization usually reduce the enzyme activity and it takes half an hour to release half of the enzyme life, which far exceeds the usual household washing time; and the system necessary for gel embedding systems such as alginate is too large to be suitable for liquid detergents

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Example 1 Used to simultaneously stabilize alkaline protease 2709 and Pseudomonas aeruginosa lipase in compound enzyme laundry detergent

[0047] Prepare entrapped alkaline protease 2709 (909.1 mg protein / g, Xingtai Spectrum Biotechnology Co., Ltd.) and Pseudomonas aeruginosa lipase (839.6 mg protein / g, provided by the Renewable Resources Laboratory of Engineering College of Jiangnan University) respectively. Vesicles, the specific steps are as follows:

[0048] (1) Dissolve 11.52 g of conjugated linoleic acid in 200 mL of sodium dihydrogen phosphate-disodium hydrogen phosphate buffer (pH 8.6, 0.01 mol / L) containing 0.25 mmol / L calcium citrate at room temperature. After mixing evenly, take half of it and add 3.17g of alkaline protease 2709, and add 3.43g of Pseudomonas aeruginosa lipase to the other half. After the two solutions were mixed evenly, each at 25 0 Under C, use a shaker to oscillate separately in the dark for 4h (shaker speed 50rpm), promptly obtain two ki...

Embodiment 2

[0056] Embodiment 2 is used in compound enzyme laundry detergent to simultaneously stabilize mucous Sailae alkaline protease and Pseudomonas aeruginosa lipase

[0057] Preparation of entrapped S. marcescens alkaline protease (930.2 mg protein / g, from S. marcescens) and Pseudomonas aeruginosa lipase (839.6 mg protein / g, provided by the Renewable Resources Laboratory of Engineering College of Jiangnan University) Vesicles, the specific steps are as follows:

[0058] (1) Dissolve conjugated linoleic acid and calcium citrate in 0.01mol / L borax buffer (pH9.18) at room temperature and mix well. The final concentration of conjugated linoleic acid is 300mmol / L, and the calcium salt The concentration is 0.15mmol / L; after mixing evenly, take 100mL of the above solution respectively, and add 5.25g mucilaginous Saib Alkaline protease and 5.81g Pseudomonas aeruginosa lipase, that is, the amount of enzyme added is 565mg protein / g Conjugated linoleic acid, after mixing evenly, each shaken w...

Embodiment 3

[0060] Example 3 Used to simultaneously stabilize neutral protease 1398 and Conn alkaline lipase in compound enzyme laundry detergent

[0061] Preparation of vesicles embedded with neutral protease 1398 (905.7 mg protein / g, Xingtai Spectrum Biotechnology Co., Ltd.) and alkaline lipase (860.3 mg protein / g, Shenzhen Dadi Kangen Biotechnology Co., Ltd.), Specific steps are as follows:

[0062] (1) Dissolve conjugated linoleic acid and calcium citrate tetrahydrate in 0.01mol / L pH 8 disodium hydrogen phosphate-citric acid buffer at room temperature, and the final concentration of conjugated linoleic acid is 240 mmol / L , the concentration of calcium salt is 0.25 mmol / L; after mixing evenly, take 100mL of the above solution respectively, add 4.04g of neutral protease 1398 and 4.26g of lipase, the amount of both enzymes added is 585mg protein / g conjugated Linoleic acid, after mixing evenly, each shaken at 25°C for 5.5 hours with a shaking table at a shaking speed of 50 rpm, respectivel...

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Abstract

The invention provides a method for simultaneously stabilizing lipase and protease in a complex enzyme laundry detergent and belongs to the technical field of daily-use chemical industry. The method includes: enabling conjugated linoleic acid to be respectively self-assembled with protease and lipase to form vesicae embedded with protease or lipase at a certain pH value and low calcium ion concentration; mixing, enabling conjugated linoleic acid on the surface layers of the vesciae to partly crosslink to obtain a complex enzyme vesica solution which can be directly used in a laundry detergent formulation after being concentrated. Lipase in the laundry detergent cannot be decomposed by protease, and enzyme vesicae can stand enzyme activity inhibition of a surfactant, so that both the lipase and the protease can maintain enzyme activity in the laundry detergent, and when in use, the laundry detergent can disintegrated and respectively release the protease and the lipase at higher calcium ion concentration like in an environment with washing water.

Description

technical field [0001] The invention belongs to the technical field of daily chemicals, and in particular relates to a method for stabilizing lipase and protease in compound enzyme laundry detergent. Background technique [0002] Laundry liquid is rich in anionic surfactants, amphoteric surfactants and nonionic surfactants, among which anionic surfactants are the main decontamination ingredients. In order to improve the washing performance of laundry detergent, it is necessary to add protease to the laundry detergent formula to help effectively remove protein stains such as blood stains, and to add lipase to facilitate the removal of grease stains; therefore, alkaline lipase and protease are compound enzymes in laundry detergent The two most commonly used enzymes. [0003] There are two problems with the stability of enzymes in compound enzyme laundry detergent. One, in liquid, proteases can degrade lipases, so they need to be isolated. Second, the stability of enzymes in...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N9/96
Inventor 方云夏咏梅魏贝闫引星
Owner JIANGNAN UNIV
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