A kind of nano gel slant culture medium and preparation method thereof

A slant culture medium and nano-gel technology, applied in the field of nano-gel slant culture medium and its preparation, can solve the problems such as affecting the growth of bacterial liquid, nutrient balance, too large difference in thickness between the upper and lower surfaces of the slant, affecting test accuracy, etc. The effect of avoiding repeated tests, delaying the setting time and shortening the test time
CN107034140BActive Publication Date: 2019-09-13句容亿格纳米材料厂
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
句容亿格纳米材料厂
Publication Date
2019-09-13

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Abstract

The invention discloses a nanogel inclined plane culture medium and a preparation method thereof and belongs to the field of microculture. Part of agar powder is replaced with part of nanogel particles and agarose gel on the basis of an ordinary inclined plane culture medium. Concretely, by mass percentage, first nanogel particles are used to replace 20-30% of agar powder, and then the agarose gel is used to replace 25%-35% of agar powder. The setting time and setting rate of the culture medium are effectively delayed, the thickness uniformity degree during inclined plane culture medium preparation is improved, the uniform distribution degree of culture medium nutrient substances is also improved, and thus accuracy of culture and subsequent test results is effectively ensured. Repeated testing is avoided, testing time is shortened, errors are reduced, and the precision degree of tests is improved. In addition, the preparation method is reasonable in design and simple and convenient to operate.
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Description

technical field

[0001] The invention relates to the field of microorganism culture, in particular to a nanogel slant medium and a preparation method thereof. Background technique

[0002] Culture medium is the nutrient substrate necessary for the growth, reproduction or accumulation of metabolites of microorganisms or animal or plant cells in the process of microbial fermentation or animal and plant cell culture. The components of a general medium include: carbon sources, nitrogen sources, minerals, and other essential substances, such as substances that cannot be synthesized by certain organisms themselves, such as certain amino acids, vitamins or nucleotides. In addition, pH buffers are often added to the medium to maintain a stable pH.

[0003] In the prior art, the solid medium is generally based on agar as the main solidified carrier and supplemented with other nutrients, but there are certain problems when it is applied to the slant medium, because the solidification ...

Claims

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