A method for determining free butyric acid in milk/dairy products
A technology for dairy products and butyric acid, applied in the field of food safety, can solve the problems of limiting accuracy and precision, increasing adsorption efficiency and separation, and many interfering substances, and achieving high precision, high accuracy and high extraction efficiency. Effect
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Embodiment 1
[0026] (1) Preparation of standard solution
[0027] Butyric acid standard solution: Accurately weigh 1 g of butyric acid (purity is chromatographic grade) to prepare 1 L of mother liquor and store it in a refrigerator at 4°C for later use. The standard solution is obtained by diluting the mother solution with an appropriate gradient, and it is freshly prepared before use.
[0028] Internal standard solution: Accurately weigh 1 g of heptanoic acid (purity is chromatographic grade), and prepare 1 L of heptanoic acid in ethanol solution with a mass volume ratio of 1 mg / ml, and the concentration of ethanol solution is 5%, and store it in a refrigerator at 4°C for later use.
[0029] (2) Sample processing
[0030] Take 5mL pasteurized milk sample A and place it in a 15mL headspace bottle, add 1.5g sodium chloride and 50μL internal standard solution, and immediately tighten the bottle cap.
[0031] Fatty acid standard sample: Weigh the butyric acid mother liquor to prepare a 55.2...
Embodiment 2
[0045] (1) Preparation of standard solution
[0046] Butyric acid standard solution: Accurately weigh 1 g of butyric acid (purity is chromatographic grade) to prepare 1 L of mother liquor and store it in a 4°C refrigerator for later use. The standard solution is obtained by diluting the mother solution with an appropriate gradient, and it is freshly prepared before use.
[0047] Internal standard solution: Accurately weigh 1 g of heptanoic acid (purity is chromatographic grade), and prepare 1 L of heptanoic acid in ethanol solution with a mass volume ratio of 1 mg / ml, and the concentration of ethanol solution is 5%, and store it in a refrigerator at 4°C for later use.
[0048] (2) Sample processing
[0049] Take 5mL of LUHT sterilized milk sample B and place it in a 15mL headspace bottle, add 2.5g of sodium chloride and 5μL of internal standard solution, and immediately tighten the bottle cap.
[0050] Fatty acid standard sample: Weigh the butyric acid mother liquor to prepa...
Embodiment 3
[0064] (1) Preparation of standard solution
[0065] Butyric acid standard solution: Accurately weigh 1 g of butyric acid (purity is chromatographic grade) to prepare 1 L of mother liquor and store it in a 4°C refrigerator for later use. The standard solution is obtained by diluting the mother solution with an appropriate gradient, and it is freshly prepared before use.
[0066] Internal standard solution: Accurately weigh 1 g of heptanoic acid (purity is chromatographic grade), and prepare 1 L of heptanoic acid in ethanol solution with a mass volume ratio of 1 mg / ml, and the concentration of ethanol solution is 5%, and store it in a refrigerator at 4°C for later use.
[0067] (2) Sample processing
[0068] Take 5mL raw milk sample C and put it in a 15mL headspace bottle, add 0.5g sodium chloride and 50μL internal standard solution, and immediately tighten the bottle cap.
[0069] Fatty acid standard sample: Weigh the butyric acid mother liquor to prepare a 55.2 mg / L standar...
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