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A polyionic liquid modified reduced graphene oxide drug-loaded doxorubicin and its preparation method and application

A technology of polyionic liquids and graphene, which is applied in pharmaceutical formulations, antineoplastic drugs, drug combinations, etc., can solve problems such as easy flocculation and aggregation, and achieve the effects of prolonging the action time, improving targeting, and high release rate

Active Publication Date: 2019-07-16
LIAONING UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, GO is easily aggregated by flocculation in saline and physiological solutions

Method used

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  • A polyionic liquid modified reduced graphene oxide drug-loaded doxorubicin and its preparation method and application
  • A polyionic liquid modified reduced graphene oxide drug-loaded doxorubicin and its preparation method and application
  • A polyionic liquid modified reduced graphene oxide drug-loaded doxorubicin and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1 Investigation of prescription technology

[0027] Preparation method: After mixing the graphene oxide (GO) solution with the PIL solution, ultrasonic reaction and stirring reaction, pH adjustment, simultaneous reduction reaction under the reducing agent, after the reaction is completed, centrifugation, washing, and drying to obtain PIL-RGO composite material . Dissolve the PIL-RGO composite material in water, add doxorubicin hydrochloride DOX aqueous solution, adjust the pH to 7-10, shading, stir the reaction, centrifuge, freeze-dry, and obtain the target product polyionic liquid modified reduced graphene oxide drug-loaded doxorubicin Vegetarian PIL-RGO / DOX.

[0028] Investigate the influence of different reaction conditions on the experimental results, and screen the optimal formulation process.

[0029] (1) The influence of the mass ratio of PIL and GO on the preparation of PIL-RGO composites

[0030] For screening, the GO solution concentration is 1 mg / ml and the...

Embodiment 2

[0049] Example 2 Polyionic liquid modified reduced graphene oxide drug-loaded doxorubicin (PIL-RGO / DOX)

[0050] (1) Preparation method:

[0051] 1. Preparation of PIL-RGO composite material: Take 100ml GO solution (1mg / ml), add dropwise to 100ml poly-1-vinyl-3-ethylimidazole bromide solution (16mg / ml) under stirring at 100rpm, and react with ultrasound Stir at 20min and 100rpm for 1h to obtain PIL-GO; adjust the pH to 10 with NaOH solution (1mol / ml), add 1.1ml hydrazine hydrate to 100℃ for simultaneous reduction reaction for 12h, after the reaction is completed, centrifuge at 10000rpm for 1h and wash with pure water. Repeat centrifugation and drying to obtain PIL-RGO composite material.

[0052] 2. Preparation of PIL-RGO / DOX: Dissolve 25 mg of PIL-RGO composite material in 50 ml of pure water, add 5 ml of DOX solution (1 mg / ml) dropwise, and adjust the pH to 10 with NaOH solution (1 mol / ml). After the reaction was shaded and stirred for 24 hours, it was centrifuged at 12000 rpm fo...

Embodiment 3

[0066] Example 3 The effect of the type of polyionic liquid (PIL) on PIL-RGO

[0067] (1) Take 100ml GO solution (1mg / ml), add dropwise to 100ml poly-1-vinyl-3-ethylimidazole bromide solution (16mg / ml) under stirring at 100rpm, and react with ultrasonic for 20min and stir for 1h at 100rpm ; Adjust the pH to 10 with NaOH solution (1mol / ml), add 1.1ml of hydrazine hydrate at 100℃ for simultaneous reduction reaction for 12h, after the reaction is completed, centrifuge for 1h at 10,000, wash with pure water, repeat centrifugation and drying to obtain the PIL-RGO composite material.

[0068] (2) Take 100ml GO solution (1mg / ml), add dropwise to 100ml poly-1-allyl-3-ethylimidazole bromide solution (16mg / ml) under stirring at 100rpm, and react with ultrasound for 20min and stir at 100rpm for reaction 1h; Adjust the pH to 10 with NaOH solution (1mol / ml), add 1.1ml of hydrazine hydrate to 100℃ for simultaneous reduction reaction for 12h, after the reaction is completed, centrifuge for 1h at...

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Abstract

The invention relates to a polymeric ionic liquid modified reduced graphene oxide loaded doxorubicin, as well as a preparation method and an application of the polymeric ionic liquid modified reduced graphene oxide loaded doxorubicin. The preparation method of the polymeric ionic liquid modified reduced graphene oxide loaded doxorubicin comprises the following steps: preparing PIL-RGO (Polymeric Ionic Liquid-Reduced Graphene Oxide); dissolving a PIL-RGO composite into water, adding a doxorubicin hydrochloride aqueous solution, adjusting the PH to neutral, stirring for a reaction under the shading condition, centrifuging to remove DOX not loaded, and freeze-drying to form the PIL-RGO composite. The carrier material is excellent in solubleness in water, and the stability is strengthened. The polymeric ionic liquid modified reduced graphene oxide loaded doxorubicin has the PH sensitive release characteristic after being loaded, the detention time of DOX in the body is prolonged, and the sustained-release effect is achieved, so that the treatment effect is strengthened. The polymeric ionic liquid modified reduced graphene oxide loaded doxorubicin can be applied to the studies on antitumor, and has excellent clinical study and application values.

Description

Technical field [0001] The invention belongs to the field of pharmaceutical preparations, and particularly relates to a polyionic liquid modified reduced graphene oxide drug-loaded adriamycin and a preparation method thereof. Background technique [0002] Graphene oxide (GO) is a two-dimensional (2D) honeycomb lattice structure with a single layer of carbon atoms in the form of sp2 hybridization. Its surface contains a large number of oxygen-containing functional groups and has good hydrophilicity. It is also GO further Functionalization provides sufficient active sites. Therefore, the research of using GO and its derivatives as enzyme, protein and drug carriers is becoming a hot spot. However, GO is easy to flocculate and aggregate in saline and physiological solutions. Therefore, there is an urgent need for functional modification to obtain graphene oxide derivatives that can stably exist in physiological solutions. [0003] Ionic Liquids are salts composed of organic cations ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/51A61K9/50A61K47/04A61K31/704A61P35/00
CPCA61K9/501A61K9/5115A61K31/704
Inventor 刘宇吴浩天陈立江李丽刘冰弥
Owner LIAONING UNIVERSITY
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