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Entomogenous fungus granules and preparation method thereof

A technology for insect-growing fungi and granules, applied in the field of insect-growing fungi granules and their preparation, can solve the problem that the surface of the granules is easily polluted by miscellaneous bacteria, and achieves the advantages of enhancing soil fertilizer supply, good activity and good insect control effect. Effect

Pending Publication Date: 2017-08-18
CHONGQING JULIXIN BIOENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The granule is first collected the spore powder of the entomogeneous fungus, then adopts the corresponding formula and process to make the Beauveria bassiana granule, and the disintegration speed of the granule and the release speed of the conidia are controlled by related auxiliaries to prolong the effective period , although it has a slow-release effect, but when controlling underground pests, the surface of the granules is easily polluted by miscellaneous bacteria

Method used

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  • Entomogenous fungus granules and preparation method thereof
  • Entomogenous fungus granules and preparation method thereof
  • Entomogenous fungus granules and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Embodiment 1: first place the 1 / 4SDAY plate (0.25% of peptone, 0.5% of yeast extract, 1.0% of glucose, 1.8% of agar) bacterial classification of scarabia anisopliae Metarhizium anisopliae (CQMa421) to be activated, then inoculate it on Cultivate 2.5 days (60 hours) at 26°C on the sterilized 1 / 4SDAY Sabouraud liquid medium to obtain liquid strains; then soak rice with a particle size of 3 mm in water for 3 hours, and drain Sterilize and cool to obtain a granular carrier; then mix the liquid strain and the granular carrier according to the mass ratio = 3:100, stir evenly, place it at 26°C for 5 days, and obtain 20 million live spores / grams of scarab metarhizium anisopliae granules.

Embodiment 2

[0023] Embodiment 2: put the 1 / 4SDAY plate (0.25% of peptone, 0.5% of yeast extract, 1.0% of glucose, 1.8% of agar) bacterial classification of scarabiana metarhizium anisopliae (CQMa421) to be activated first, then inoculate it on the Place the sterilized 1 / 4SDAY Sabouraud liquid culture medium at 26°C for 2.5 days to obtain liquid strains; then soak the rice with a particle size of 3mm in water for 3 hours, drain and sterilize Treat and cool to obtain a granular carrier; then mix the liquid bacteria and the granular carrier according to the mass ratio = 3:100, stir evenly and place it at 26°C for 7 days, and obtain scarabs with 0.5 billion live spores per gram after drying Metarhizium anisopliae granules.

Embodiment 3

[0024] Embodiment 3: first place the 1 / 4SDAY plate (0.25% of peptone, 0.5% of yeast extract, 1.0% of glucose, 1.8% of agar) bacterial classification of scarabia anisopliae Metarhizium anisopliae (CQMa421) to be activated, then inoculate it on Put the sterilized 1 / 4SDAY Sabouraud liquid culture medium at 26°C for 2.5 days to obtain liquid strains; then soak the barley in water for 4 hours, drain and sterilize, and cool to obtain Granular carrier; then mix the liquid strain and the granular carrier according to the mass ratio = 3:100, stir evenly and then culture it at 26°C for 11 days, and obtain 100 million live spores / gram of Metarhizium anisopliae granules after drying .

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Abstract

The invention provides entomogenous fungus granules. The entomogenous fungus granules comprise granular carriers and entomogenous fungus spores, wherein the granular carriers are fermented natural crop granules or artificially synthesized nutrient granules, and the required granular carriers are intact in surfaces; the entomopathogenic fungus spores are activated entomogenous fungus spores, and the entomogenous fungus spores are attached (wrapped) to the surfaces of the granular carriers. The entomogenous fungus granules disclosed by the invention do not contain any additives of adhesives, lubricants, dispersants or the like, and comprise the granular carriers and the entomogenous fungus spores in reproductive statuses instead of being further prepared by mixing spore powder. The surfaces of the entomogenous fungus granules are not easily contaminated by miscellaneous bacteria after applied to the inner part of soil, and the entomogenous fungus granules inside the soil have the persistent period lasting for 2 months.

Description

technical field [0001] The invention relates to an insect-growing fungus granule and a preparation method thereof, which is specifically used for preventing and controlling underground pests such as grubs, cutworms, and needleworms in soil. Background technique [0002] Entomogenic fungi refer to fungi that can parasitize insects such as scarabs, spiders, termites, locusts, grubs, cutworms, and needle worms, and can cause insects to become diseased and dead. Deficient bacteria (such as Beauveria bassiana, Metarhizium anisopliae). Based on their pathogenic properties, entomogenic fungi (especially Beauveria bassiana and Metarhizium anisopliae) are usually used to develop biopesticide preparations such as powders, oil suspensions, and granules. [0003] CN104770397A discloses a Beauveria bassiana powder, which comprises 1-5% of Beauveria bassiana spore powder, 0.5-2% of an anti-ultraviolet protection agent, and 94-98% of a filler, and each component is in mass percent. CN122...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05G3/00C05G3/02C05G3/04C05G5/00A01N25/12A01N63/04A01P7/04C05G3/60C05G3/80
CPCA01N25/12A01N63/30C05F11/00C05G3/60C05G3/80C05G5/30C05F11/08
Inventor 夏玉先彭国雄
Owner CHONGQING JULIXIN BIOENG
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