Method for simultaneously detecting sunset yellow and malachite green in aquatic product

A malachite green and sunset yellow technology, applied in the field of analysis and detection, achieves the effects of easy operation, improved detection sensitivity, and a wide range of applications

Inactive Publication Date: 2017-08-18
SHANGHAI APPLIED TECHNOLOGIES COLLEGE
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, the Chinese patent (publication number CN 104977286 A) uses gold nanoparticles as the SERS subs

Method used

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  • Method for simultaneously detecting sunset yellow and malachite green in aquatic product
  • Method for simultaneously detecting sunset yellow and malachite green in aquatic product
  • Method for simultaneously detecting sunset yellow and malachite green in aquatic product

Examples

Experimental program
Comparison scheme
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Example Embodiment

[0026] Example 1: Electrochemical enrichment-surface enhanced Raman technology to detect sunset yellow and malachite green

[0027] (1) Preparation of reduced graphene oxide

[0028] The graphene oxide was prepared by the improved Hummers method, and 0.5mg / mL graphene oxide flocculation was obtained at a constant volume. 5mL of graphene oxide was taken in a distillation flask, 5μL of hydrazine hydrate (35%) was added as a reducing agent, and 100μL of ammonia (28 %), stir well, and reflux at 95°C for 1 h. Allow to stand at room temperature, centrifuge at 10000 rpm for 20 min, take the lower layer liquid to a constant volume to obtain a 0.5 mg / mL reduced graphene oxide solution.

[0029] (2) Preparation of gold nanorods by seed method

[0030] a. Preparation of gold nano seed solution: at room temperature (25-28°C), prepare 9.75ml 0.1mol / L cetyltrimethylammonium bromide aqueous solution, stir evenly until transparent, add 0.25ml 0.01mol / L dropwise Chloroauric acid aqueous solution, af...

Example Embodiment

[0047] Example 2: Detection of sunset yellow and malachite green in aquatic products

[0048] figure 1 A flowchart of sunset yellow and malachite green in aquatic products in an embodiment of the present invention is schematically given, and the detection method includes the following steps:

[0049] (1) Pretreatment of fish samples

[0050] The specific steps for the extraction and purification of malachite green and its metabolites in fish meat are as follows: Weigh 2.00±0.02g fish meat into a 50ml centrifuge tube, add 500μL of 9.5g / L hydroxylamine hydrochloride solution and place it in the dark for 10 minutes, then vortex Shake to spread the meat evenly. Add 10ml of acetonitrile and 1.0±0.1g of anhydrous magnesium sulfate and vortex vigorously for 1min, then add 4.0±0.1g of neutral alumina and place it in a shaker and shake for 10min at 250rpm. After that, the sample was centrifuged at 3220 rpm for 5 min, and the supernatant was transferred to a 15 mL centrifuge tube with a pipe...

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Abstract

The invention discloses a method for simultaneously detecting sunset yellow and malachite green in an aquatic product. The method comprises the following steps: (1) preparing a reduced graphene (RGO) nano material by virtue of a hydrothermal method; (2) synthesizing a gold nano rod (GNRs) by virtue of a seed crystal growth method; (3) respectively adsorbing RGO, GNRs, SY and MG in the solution on a screen printing electrode by adopting an electrochemical concentrating method; and (4) detecting a Raman spectral signal of a substance on the surface of the screen printing electrode, so that the SY and MG in the aquatic product can be qualitatively and quantitatively analyzed and detected. By adopting the electrochemical enrichment-SERS detection technology, the method not only has the characteristics of rapidness in analysis, high sensitivity, less consumption of samples, wide application range, simplicity in operation, convenience in carrying and the like, but also has excellent selectivity; and moreover, marking, separation and purification is needed, the sunset yellow and the malachite green in the aquatic product can be simultaneously analyzed and detected, and the detection limit is low.

Description

technical field [0001] The invention belongs to the technical field of analysis and detection, and in particular relates to a method for simultaneously detecting sunset yellow and malachite green in aquatic products. Background technique [0002] Sunset yellow (SY) is mainly used for coloring food and medicine, but its usage is strictly limited. If a person consumes a large amount of food with excessive pigment content such as sunset yellow for a long time or at one time, it may cause symptoms such as allergies and diarrhea. When the intake is too large and exceeds the liver load, it will accumulate in the body and cause certain damage to the kidneys and liver. . Malachite Green (MG) is a toxic triphenylmethane chemical, which is not only a dye, but also a fungicide, bactericide, and parasite-killer drug. Therefore, it can be used as a fungicide for fish, etc., but long-term excessive use may cause Carcinogenic, teratogenic and mutagenic. Malachite green is also listed as...

Claims

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Application Information

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IPC IPC(8): G01N21/65G01N1/40
CPCG01N21/658G01N1/40G01N2001/4038
Inventor 李丹邓维段化珍马亚丹
Owner SHANGHAI APPLIED TECHNOLOGIES COLLEGE
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