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Method for simultaneously detecting sunset yellow and malachite green in aquatic product

A malachite green and sunset yellow technology, applied in the field of analysis and detection, achieves the effects of easy operation, improved detection sensitivity, and a wide range of applications

Inactive Publication Date: 2017-08-18
SHANGHAI APPLIED TECHNOLOGIES COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, the Chinese patent (publication number CN 104977286 A) uses gold nanoparticles as the SERS substrate to analyze and detect MG, but there are few reports on the simultaneous analysis and detection of SY and MG

Method used

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  • Method for simultaneously detecting sunset yellow and malachite green in aquatic product
  • Method for simultaneously detecting sunset yellow and malachite green in aquatic product
  • Method for simultaneously detecting sunset yellow and malachite green in aquatic product

Examples

Experimental program
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Effect test

Embodiment 1

[0026] Example 1: Detection of Sunset Yellow and Malachite Green by Electrochemical Enrichment-Surface Enhanced Raman Technology

[0027] (1) Preparation of reduced graphene oxide

[0028] Utilize improved Hummers method to prepare graphene oxide, constant volume obtains 0.5mg / mL graphene oxide flocculation, take 5mL graphene oxide in the distillation flask, add 5 μ L hydrazine hydrate (35%) as reducing agent, add 100 μ L ammoniacal liquor (28 %), fully stirred, and refluxed at 95°C for 1h. Stand at room temperature, centrifuge at 10,000 rpm for 20 min, remove the lower layer and make up to volume to obtain a 0.5 mg / mL reduced graphene oxide solution.

[0029] (2) Preparation of gold nanorods by seed crystal method

[0030] a. Preparation of gold nano seed solution: at room temperature (25-28°C), prepare 9.75ml 0.1mol / L hexadecyltrimethylammonium bromide aqueous solution, stir evenly until transparent, add dropwise 0.25ml 0.01mol / L Chlorauric acid aqueous solution, after it...

Embodiment 2

[0047] Embodiment 2: detection of sunset yellow and malachite green in aquatic products

[0048] figure 1 The flow chart of sunset yellow and malachite green in the aquatic product of the embodiment of the present invention is schematically provided, and the detection method comprises the following steps:

[0049] (1) Pretreatment of fish samples

[0050] The specific steps for the extraction and purification of malachite green and its metabolites in fish meat are as follows: Weigh 2.00±0.02g fish meat into a 50mL centrifuge tube, add 500μL 9.5g / L hydroxylamine hydrochloride solution and place it in the dark for 10min, then vortex Shake to distribute the meat evenly. After adding 10mL of acetonitrile and 1.0±0.1g of anhydrous magnesium sulfate, vigorously vortex for 1min, then add 4.0±0.1g of neutral alumina, put it in a shaker and vibrate at 250rpm for 10min. Afterwards, the sample was centrifuged at 3220rpm for 5min, and the supernatant was pipetted into a 15mL centrifuge...

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Abstract

The invention discloses a method for simultaneously detecting sunset yellow and malachite green in an aquatic product. The method comprises the following steps: (1) preparing a reduced graphene (RGO) nano material by virtue of a hydrothermal method; (2) synthesizing a gold nano rod (GNRs) by virtue of a seed crystal growth method; (3) respectively adsorbing RGO, GNRs, SY and MG in the solution on a screen printing electrode by adopting an electrochemical concentrating method; and (4) detecting a Raman spectral signal of a substance on the surface of the screen printing electrode, so that the SY and MG in the aquatic product can be qualitatively and quantitatively analyzed and detected. By adopting the electrochemical enrichment-SERS detection technology, the method not only has the characteristics of rapidness in analysis, high sensitivity, less consumption of samples, wide application range, simplicity in operation, convenience in carrying and the like, but also has excellent selectivity; and moreover, marking, separation and purification is needed, the sunset yellow and the malachite green in the aquatic product can be simultaneously analyzed and detected, and the detection limit is low.

Description

technical field [0001] The invention belongs to the technical field of analysis and detection, and in particular relates to a method for simultaneously detecting sunset yellow and malachite green in aquatic products. Background technique [0002] Sunset yellow (SY) is mainly used for coloring food and medicine, but its usage is strictly limited. If a person consumes a large amount of food with excessive pigment content such as sunset yellow for a long time or at one time, it may cause symptoms such as allergies and diarrhea. When the intake is too large and exceeds the liver load, it will accumulate in the body and cause certain damage to the kidneys and liver. . Malachite Green (MG) is a toxic triphenylmethane chemical, which is not only a dye, but also a fungicide, bactericide, and parasite-killer drug. Therefore, it can be used as a fungicide for fish, etc., but long-term excessive use may cause Carcinogenic, teratogenic and mutagenic. Malachite green is also listed as...

Claims

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Application Information

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IPC IPC(8): G01N21/65G01N1/40
CPCG01N21/658G01N1/40G01N2001/4038
Inventor 李丹邓维段化珍马亚丹
Owner SHANGHAI APPLIED TECHNOLOGIES COLLEGE
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