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Method for determining contents of morphine and glycyrrhizic acid in compound liquorice tablet

A technology of compound licorice tablets and glycyrrhizic acid, which is applied to measuring devices, instruments, scientific instruments, etc., can solve the problems of complicated operation, low precision, long time consumption, etc. Effect

Inactive Publication Date: 2017-08-18
SHENYANG NO 1 PHARMA FACTORY DONGBEI PHARMA GRP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In "Chinese Pharmacopoeia" (2015 edition), morphine and glycyrrhizic acid, the functional ingredients of compound licorice tablets, were detected by two completely different liquid chromatography systems. For the determination of morphine, the sample was first extracted in a solid-phase extraction column, and then dissolved in octane Based on silane-bonded silica gel column detection, the method is complex in operation, low in precision, time-consuming, and high in cost. Therefore, developing a detection method for the content of compound licorice tablets has always been a new subject to be solved urgently.

Method used

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  • Method for determining contents of morphine and glycyrrhizic acid in compound liquorice tablet
  • Method for determining contents of morphine and glycyrrhizic acid in compound liquorice tablet
  • Method for determining contents of morphine and glycyrrhizic acid in compound liquorice tablet

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Experimental program
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Effect test

Embodiment 1

[0047] 1. Chromatographic conditions

[0048] Chromatographic column: SUPERIOREX (Shiseido) MG C18 250×4.6mm 5μm, column temperature: 30℃~40℃, flow rate: 1.3ml / min~1.7ml / min, detection wavelength: 210nm, 250nm, injection volume: 10μl~25μl .

[0049] 2. Solution Preparation

[0050] 2.1 Mobile phase: phosphate buffer and acetonitrile

[0051] 2.2 Phosphate buffer: Take 6.8g of potassium dihydrogen phosphate, dissolve it in an appropriate amount of water, add 1.1g of sodium heptanesulfonate and 2ml of triethylamine, adjust the pH to 4.9±0.1 with 20% phosphoric acid solution, and dilute to 1000ml with water.

[0052] 2.3 Diluent: Take 50ml of glacial acetic acid, add water to 500ml, mix well, add 500ml of methanol, shake well, and you get it.

[0053] 2.4 Test solution: Take 20 tablets of this product, accurately weigh, grind finely, accurately weigh 0.15881g, 0.15675g, 0.15656g, put them in 50ml measuring bottles respectively, add appropriate amount of diluent, ultrasonic for...

Embodiment 2

[0104] The selection of the composition ratio in the mobile phase during concentration and gradient elution of embodiment two

[0105] Other conditions are the same as in Example 1, using mobile phase phosphate buffer and acetonitrile concentration, gradient elution, and variable wavelength method;

[0106] 0-4 minutes after the start of elution, phosphate buffer 86%, acetonitrile 14%, wavelength 210nm; 4-4.01 minutes after the start of elution, phosphate buffer 86%→73%, acetonitrile 14%→27%, wavelength 250nm; 4.01-28 minutes after the start of elution, phosphate buffer 73%, acetonitrile 27%, wavelength 250nm; 28-28.01 minutes after the start of elution, phosphate buffer 73%→50%, acetonitrile 27%→50% , wavelength 250nm; 28.01-47 minutes after the start of elution, phosphate buffer 50%, acetonitrile 50%, wavelength 250nm; 47-47.01 minutes after the start of elution, phosphate buffer 50% → 86%, acetonitrile 50% → 14%, wavelength 210nm; 47.01-65 minutes after the start of elutio...

Embodiment 3

[0108] The selection of embodiment three mobile phase pH value

[0109] Other conditions are the same as in Example 1, using phosphate buffer and acetonitrile as the mobile phase. Phosphate buffer solution: Take 6.8g of potassium dihydrogen phosphate, dissolve it in an appropriate amount of water, add 1.1g of sodium heptanesulfonate and 2ml of triethylamine, adjust the pH to 5.1 with 20% phosphoric acid solution, and dilute to 1000ml with water.

[0110] The chromatographic peak of glycyrrhizic acid in the test solution is interfered by adjacent impurity peaks, so it cannot be detected.

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Abstract

The invention relates to a method for determining the contents of morphine and glycyrrhizic acid in a compound liquorice tablet in the field of analysis. The method comprises the following chromatographic conditions: a chromatographic column is a C18 column utilizing octadecylsilane chemically bonded silica as a filling agent, and the particle size is 5 microns; the column temperature is 30-40 DEG C; the flow speed is 1.3ml / min-1.7ml / min; the detection wavelengths are 210nm and 250nm; the sample feeding amount is 10-25 microliters; and flow phases are a phosphate buffer solution and ethanol. The method is used for detecting the contents of morphine and glycyrrhizic acid in the compound liquorice tablet by virtue of an HPLC method and has the advantages that a reagent is easily available, the operation is simple and convenient, the time is short, the precision is high, the detection cost is low, and the like.

Description

technical field [0001] The invention relates to a method for detecting the contents of morphine and glycyrrhizic acid in compound licorice tablets in the field of drug analysis. Background technique [0002] Compound Liquorice Tablets, the English name is Compound Liquorice Tablets, this product is a brown tablet; it has a special smell, a sweet taste, and is easy to absorb moisture. Compound licorice tablet is recorded in the second part of the 2015 edition of the "Chinese Pharmacopoeia", mainly composed of licorice extract powder, opium powder, camphor, star anise oil, sodium benzoate, etc. Common medicine for cough. Among them, licorice extract powder is a protective antitussive and expectorant; opium powder has a strong antitussive effect; camphor and star anise oil can stimulate bronchial mucosa, reflexively increase gland secretion, dilute sputum, and make phlegm easy to cough out; sodium benzoate is a preservative. The above ingredients form a compound preparation,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 刘宇李翱赵鼎
Owner SHENYANG NO 1 PHARMA FACTORY DONGBEI PHARMA GRP
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