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Method for extracting cellulose compound enzyme from agricultural by-products

A technology for cellulose composite enzymes and agricultural by-products, which is applied in the directions of microorganism-based methods, biochemical equipment and methods, enzymes, etc., can solve the problem of being unsuitable for large-scale industrial production, reducing cellulase production, and low cellulase efficiency. and other problems, to achieve the effect of being conducive to resource recycling, good repeatability and simple operation

Inactive Publication Date: 2017-08-22
信阳学院
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  • Application Information

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Problems solved by technology

However, the efficiency of cellulase prepared solely from fungus chaff is low, and the enzyme activity is not high, so it is not suitable for large-scale industrial production.
Patent CN201310431838.1 discloses a production method of cellulase, which uses lactic acid bacteria and compound mold two-stage fermentation method to prepare cellulase, but the process is relatively cumbersome, and the lactic acid produced by lactic acid bacteria in the first-stage fermentation is harmful to Aspergillus fermented by the second-stage fermentation. Has an inhibitory effect and reduces the production of cellulase

Method used

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Examples

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Effect test

example 1

[0019] The chaff of Grifola frondosa after producing two crops of fruiting bodies was selected and dried at a constant temperature of 65°C for 8 hours. Fresh bran was added to the dried chaff with a mass ratio of 1:1.5, the ratio of material to water was 1:1, and the pH was adjusted to 7.2. Nutritious comprehensive microbial culture medium. The Bacillus subtilis was activated, cultured at a constant temperature of 37°C for 48 hours in a 1% inoculum culture medium, and a cellulase-rich fermentation product was obtained. Add physiological saline to the fermentation product, shake the glass bead flask for 15 minutes, centrifuge at 5000rpm / min for 10 minutes, take the supernatant, add saturated ammonium sulfate to precipitate the protein, ultrafilter, freeze and vacuum-dry the enzyme solution to obtain the cellulose compound Enzyme powder. The obtained cellulose compound enzyme includes cellulase, xylanase and lignin peroxidase.

example 2

[0021] The chaff of Grifola frondosa after two crops of fruiting bodies was selected and dried at a constant temperature of 80°C for 6 hours, fresh bran was added to the dried chaff with a mass ratio of 1:2, the ratio of material to water was 1:1.25, and the pH was adjusted to 7.2 to produce Nutritious comprehensive microbial culture medium. Activate Bacillus subtilis, inoculate it into the culture medium prepared according to the step of 2% inoculum, and cultivate it at a constant temperature of 32°C for 48 hours to obtain a cellulase-rich fermentation product. Add physiological saline to the fermentation product, shake the glass bead flask for 25 minutes, centrifuge at 3500rpm / min for 20 minutes, take the supernatant, add saturated ammonium sulfate to precipitate protein, ultrafilter, freeze and vacuum-dry the enzyme solution to obtain cellulose complex enzyme powder. The obtained cellulose compound enzyme includes cellulase, xylanase and lignin peroxidase.

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Abstract

The invention provides a method for extracting cellulose composite enzyme from agricultural by-products, which specifically includes pre-processing edible fungus chaff, and using Bacillus subtilis solid-state fermentation to produce cellulose composite enzyme powder. The invention has extensive raw materials, low cost, simple method and operation, effectively utilizes low-molecular-weight nutrients in agricultural by-products to produce industrial enzymes, turns waste into treasure, and has strong vitality of Bacillus subtilis, avoiding the problem of miscellaneous bacteria infection in the traditional industrial enzyme preparation process. , has a strong promotion value.

Description

technical field [0001] The invention belongs to the technical field of industrial enzyme production, and in particular relates to a method for obtaining cellulose compound enzyme by utilizing agricultural by-products. Background technique [0002] With the increase of people's demand for edible fungi and the promotion and popularization of edible fungus technology, a large amount of waste culture medium-bacteria chaff after the production of edible fungi has accumulated. Mushroom chaff is a mixture composed of polyhydrocarbon sugars produced by the decomposition of cellulose, hemicellulose, and lignin from the mycelium residues of edible fungi. Among them, the crude protein, fat and small molecular weight sugars have been significantly improved, and the secondary products produced in the process of bacterial metabolism have accumulated to a certain extent, and the nutritional components are comprehensive, so it can be widely used in microbial cultivation to produce animal fe...

Claims

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Application Information

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IPC IPC(8): C12N9/42C12N9/08C12R1/125
CPCC12N9/2437C12N9/0065C12N9/248C12Y111/01014
Inventor 李欢高进勇余炎炎臧明丽鲁有强
Owner 信阳学院
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