Method for extracting cellulose compound enzyme from agricultural by-products
A technology for cellulose composite enzymes and agricultural by-products, which is applied in the directions of microorganism-based methods, biochemical equipment and methods, enzymes, etc., can solve the problem of being unsuitable for large-scale industrial production, reducing cellulase production, and low cellulase efficiency. and other problems, to achieve the effect of being conducive to resource recycling, good repeatability and simple operation
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[0018] Example 1
[0019] The mushroom bran of Grifola frondosa produced after two crops of fruiting bodies was used for drying at a constant temperature of 65°C for 8 hours. Fresh bran was just added to the dried mushroom bran in a mass ratio of 1:1.5, the material-water ratio was 1:1, and the pH was adjusted to 7.2. A nutritionally complete microbial culture medium. Bacillus subtilis was activated, and cultured at a constant temperature of 37°C for 48 hours according to a 1% inoculum volume medium to obtain a fermentation product rich in cellulase. Add physiological saline to the fermentation product, shake the glass bead shaker for 15 minutes, centrifuge at 5000 rpm / min for 10 minutes, take the supernatant, add saturated ammonium sulfate to precipitate the protein, ultrafiltration, and freeze the enzyme liquid for vacuum drying to obtain the cellulose composite Enzyme powder. The resulting cellulose complex enzymes include cellulase, xylanase and lignin peroxidase.
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[0020] Example 2
[0021] The mushroom bran of Grifola frondosa produced after two crops of fruiting bodies was used for drying at a constant temperature of 80°C for 6 hours. Fresh bran was just added to the dried mushroom bran in a mass ratio of 1:2, the material-water ratio was 1:1.25, and the pH was adjusted to 7.2. A nutritionally complete microbial culture medium. Activated Bacillus subtilis was inoculated into the step-prepared medium according to 2% inoculation amount, and cultivated at a constant temperature of 32°C for 48 hours to obtain a fermentation product rich in cellulase. Add physiological saline to the fermentation product, shake the glass bead shaker for 25min, centrifuge at 3500rpm / min for 20min, take the supernatant, add saturated ammonium sulfate to precipitate the protein, ultrafiltration, and freeze the enzyme solution for vacuum drying to obtain the cellulose composite enzyme powder. The resulting cellulose complex enzymes include cellulase, xylanase ...
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