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Method for manufacturing allulose-containing sweetener composition

A manufacturing method and technique of psicose are applied in the field of manufacturing sweetener compositions containing glucose, fructose and psicose, which can solve problems such as difficulties, unresearched control factors, difficulty in enzymatic reaction control, etc. The effect of less investment burden

Inactive Publication Date: 2017-08-29
MATSUTANI CHEM INDS CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in Patent Document 3, studies on glucose concentration and reaction temperature are limited to control factors for increasing the content ratio of false fructose, and there is no study on other control factors.
In addition, in Patent Document 3, the immobilized glucose isomerase and the recombinant microorganism with pseudofructose epimerase activity immobilized on alginate were packed into different columns for use, but this reflects that: due to immobilization The physical properties of the two enzymes are different from each other, so it is difficult to fill the two enzymes homogeneously in the same column; and it is difficult to control the enzyme reaction when packed in the same column

Method used

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  • Method for manufacturing allulose-containing sweetener composition
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  • Method for manufacturing allulose-containing sweetener composition

Examples

Experimental program
Comparison scheme
Effect test

manufacture example 1

[0102] Preparation of immobilized enzyme and determination of immobilized enzyme activity

[0103] Commercially available immobilized glucose isomerase was obtained from Nagase ChemteX Corporation (product name: Suitaze GN). The product is a granular preparation obtained by immobilizing microorganisms having glucose isomerase activity on a carrier, and the specific activity after swelling is 110.43 U / ml.

[0104] In the case of independently preparing the immobilized glucose isomerase, it is carried out through the following steps. First, 50 ml of ion exchange resin (manufactured by Purolite Corporation, trade name: PUROLITE A103S) was packed into a column, washed with 50 mM phosphate buffer (pH 8.0) containing 2 mM magnesium sulfate, and then isomerized liquid glucose containing 4500 U Glucose isomerase was adsorbed by circulating 500 ml of 50 mM phosphate buffer solution (pH 8.0) containing 2 mM magnesium sulfate (manufactured by Dow Chemical Company, product name: Spezym...

Embodiment 1

[0107] The glucose solution was passed through the column packed with immobilized glucose isomerase and immobilized psicose epimerase Confirmation of obtained sweetener composition (under the condition of space velocity SV=0.2)

[0108] Commercially available immobilized glucose isomerase prepared in Production Example 1 or immobilized immobilized glucose isomerase and immobilized psicose epimerase prepared in-house were prepared in accordance with the amount and activity ratio shown in Table 1. Mix uniformly by method, and fill in a glass column with a jacketed inner diameter of 32 mm and a length of 300 mm.

[0109] [Table 1]

[0110] Activity ratio of the mixed 2 immobilized enzymes

[0111]

[0112] A 35% by mass glucose solution whose pH was adjusted to 7.8 to 8.0 by adding 2 mM magnesium sulfate and sodium carbonate was prepared. The glucose solution was continuously passed through the column in an upward flow while controlling so that the jacket temperature bec...

Embodiment 2

[0118] Effects of the mixing ratio of immobilized glucose isomerase and immobilized allulose epimerase on glucose, fructose and allulone Influence of sugar composition ratio (under the condition of space velocity SV=0.3~0.4)

[0119] The commercially available immobilized glucose isomerase prepared in Production Example 1 or the immobilized immobilized glucose isomerase and immobilized psicose epimerase prepared in-house were prepared in accordance with the amount and activity ratio shown in Table 3. Mix uniformly by method, and fill in a glass column with a jacketed inner diameter of 32 mm and a length of 300 mm.

[0120] [table 3]

[0121] Activity ratio of the mixed 2 immobilized enzymes

[0122]

[0123] A 35% by mass glucose solution whose pH was adjusted to 7.8 to 8.0 by adding 2 mM magnesium sulfate and sodium carbonate was prepared. The glucose solution was continuously passed through the column in an upward flow while controlling the jacket temperature to be ...

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Abstract

The purpose of the present invention is to provide a technology whereby, in a method for manufacturing a sweetener composition containing glucose, fructose and allulose, said method comprising treating glucose with glucose isomerase and allulose epimerase, the content of allulose in the sweetener composition is increased. A sweetener composition containing glucose, fructose and allulose and having a high allulose content can be continuously manufactured at a high efficiency by immobilizing glucose isomerase and allulose epimerase, packing the same into a column so as to give an activity ratio of the immobilized glucose isomerase to the immobilized allulose epimerase of 1.49:1-5.61:1, and then passing a glucose solution through the column.

Description

technical field [0001] The present invention relates to a method for enzymatically producing a sweetener composition comprising glucose, fructose and allulose by using glucose as a raw material. More specifically, the present invention relates to the efficient production of glucose, fructose, and psicose, and a Method for sweetener composition with high ratio of ketose. Background technique [0002] Isomerized sugar is a sweetener mainly composed of glucose and fructose produced by enzymatically hydrolyzing gelatinized starch to obtain a glucose solution and causing glucose isomerase to act on it (isomerization reaction). The isomerization reaction by glucose isomerase is an equilibrium reaction, and the ratio of fructose contained in the isomerized sugar is usually 46% to 42%. Furthermore, in order to eliminate the lack of sweetness of isomerized sugar, refined fructose (fructose obtained by chromatographically separating the fructose contained in the above-mentioned isom...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/02A23L27/00C07H1/06C07H3/02
CPCA23L27/33C07H1/06C07H3/02C12Y501/03C12Y503/01005C13K13/00C12N9/90C12P19/02C12P19/24C12Y501/00C12Y503/01018C12N11/082A23V2002/00
Inventor 岛田研作P·K·古洛帕利新谷知也高冈晴造
Owner MATSUTANI CHEM INDS CO LTD
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