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Production method of high-purity black rice proteins

A production method and high-purity technology, applied in the production field of high-purity black rice protein, can solve the problems of technical difficulty in extracting black rice protein, difficult to separate, and little research, and achieve low cost, increased added value, and nutritional value. high effect

Inactive Publication Date: 2017-09-01
上海起林投资中心(有限合伙)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The protein content in black rice is 11.5-15.2% (dry basis), but as a kind of brown rice, black rice has harder rice grains, and the starch granules and protein granules in the endosperm cells are very small, tightly combined and difficult to separate
Therefore, the technical difficulty of extracting black rice protein from black rice is relatively large, and the cost is higher, and previous studies on it are seldom

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Take black japonica rice, remove foreign matter such as sand, stone and iron filings, and soak in water for 8 hours according to the solid-liquid weight ratio of 1:2-1:4. The soaked black rice is pulverized with a colloid mill to make the fineness of the black rice pulp reach 300-400 mesh. Add water and NaOH solution at 1:6 according to the weight ratio of solid to liquid in black rice slurry, control the final concentration of NaOH to 0.05mol / L, and continuously stir and extract at a temperature of 30°C for 2h. After the extraction is completed, the alkali extraction solution is quickly separated and washed by a hydrocyclone, and the light phase protein solution is collected. Transfer the protein liquid into the stirring and settling tank, adjust the pH value to 4.5-4.8 with hydrochloric acid, stir at room temperature for 12 hours, so that the black rice protein is fully precipitated, and use a plate and frame filter press to separate the filter cake, and dry it in an ...

Embodiment 2

[0023] Take black indica rice, remove foreign matter such as sand, stone and iron filings, and soak in water for 6 hours according to the solid-liquid weight ratio of 1:2-1:4. The soaked black rice is pulverized in circulation with an ultrafine pulverizer, so that the fineness of the black rice slurry reaches 300-400 mesh. Add water and NaOH solution according to the solid-to-liquid weight ratio of 1:6 in the black rice slurry, control the final concentration of NaOH to 0.05mol / L, and continuously stir and extract at a temperature of 45°C for 4h. After the extraction is completed, the alkali extraction solution is quickly separated and washed by a hydrocyclone, and the light phase protein solution is collected. Transfer the protein liquid into the stirring and settling tank, adjust the pH value to 4.5-4.8 with citric acid, stir at room temperature for 12 hours, so that the black rice protein is fully precipitated, and use a plate and frame filter press to separate the filter c...

Embodiment 3

[0025] Take black indica rice, remove foreign matter such as sand, stone and iron filings, and soak in water for 4 hours according to the solid-liquid weight ratio of 1:2-1:4. The soaked black rice is pulverized and processed with a high-pressure homogenizer, so that the fineness of the black rice slurry reaches 200-300 mesh. Add water and NaOH solution according to the solid-to-liquid weight ratio of 1:8 in the black rice slurry, control the final concentration of NaOH to 0.05mol / L, and continuously stir and extract at a temperature of 25°C for 3h. After the extraction is completed, the alkali extraction solution is quickly separated and washed by a hydrocyclone, and the light phase protein solution is collected. Transfer the protein liquid into the stirring and settling tank, adjust the pH value to 4.5-4.8 with oxalic acid, stir at room temperature for 8 hours, so that the black rice protein is fully precipitated, separate the filter cake with a disc centrifuge, and dry it w...

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Abstract

The present invention relates to a production method of high-purity black rice proteins. The production method comprises the following steps: a sand removing and magnetic separating technology is used to remove foreign matters in black rice, water is added according to a solid to liquid weight ratio of (1:2)-(1:4), and the black rice is soaked for a soaking time of 4-8 hours; the soaked black rice is crushed and a fineness of black rice slurry is 200-400 meshes; water and a NaOH solution are added into the black rice slurry with a solid to liquid weight ratio controlled at (1:6)-(1:8), and a continuous stirring and extracting is conducted at an extraction temperature of 25-45 DEG C and for an extraction time of 2-4 h; a cyclone hydraulic separator is used to conduct a rapid separating and washing of an alkali extracted solution, and a light phase protein solution is collected; a pH value of the protein solution is adjusted to be 4.5-4.8 using acids, a stirring is conducted for 8-12 h, the black rice proteins fully precipitate out, filtering cake is obtained by a separation, the obtained materials are dried, the dried materials are sterilized, and the sterilized materials are crushed and screened to obtain the finished products. The production method improves an extraction yield of the black rice proteins and also ensures a high content of anthocyanins in the black rice proteins.

Description

[0001] [technical field] [0002] The invention relates to the technical field of black rice protein extraction, in particular to a production method of high-purity black rice protein. [0003] [Background technique] [0004] Black rice is a treasure in rice, known as "tribute rice", "medicine rice" and "longevity rice". It has special nutritional value and is a precious and unique rice seed resource in my country. It is recorded in "Compendium of Materia Medica" that it has the effects of nourishing yin and tonifying kidney, invigorating spleen and warming liver, improving eyesight and promoting blood circulation. Using it as medicine has a very good effect on dizziness, anemia, white hair, eye diseases and so on. The main nutrients in the black rice include (brown rice): in terms of dry matter, it contains 8.5-12.5% ​​of crude protein, 2.7-3.8% of crude fat, 75-84% of carbohydrates, and 1.7-2% of crude ash. The inorganic salts such as manganese, zinc and copper contained in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23J1/12A23J3/14
CPCA23J1/12A23J3/14A23V2002/00A23V2250/5482
Inventor 蒋国强陈键董生福朱磊
Owner 上海起林投资中心(有限合伙)
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