Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A production process for extracting l-tryptophan from fermentation broth

A production process and tryptophan technology, applied in organic chemistry and other directions, can solve the problems of low tryptophan solubility, reduced product yield, and high cost, and achieve the effects of improving extraction yield, saving energy, and reducing steam consumption.

Active Publication Date: 2020-08-21
TIANJIN UNIV OF SCI & TECH
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it is not only expensive, but the small pore size is very easy to block, and because the solubility of tryptophan is very low, it is easy to be trapped by the membrane after crystallization, resulting in product loss and reduced product yield.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] a. Heat and inactivate the tryptophan fermentation broth, remove bacteria by microfiltration membrane filtration, take 1L of the filtrate, the measured tryptophan concentration is 25.4g / L, and enter the anion decolorization resin (special decolorization resin SQD-817) For decolorization, the column loading rate was 4BV / h, and then washed with 1L deionized water to collect 1.5L of the effluent; the tryptophan content of the effluent was measured to be 16.7g / L, and the tryptophan loss rate was 1.4%;

[0019] b. Add 1.25 g of activated carbon (powdered, 5% of the total mass of tryptophan in the eluent) to the eluent obtained in step a, decolorize for 15 minutes at a temperature of 45° C., and obtain the decolorized solution by suction filtration;

[0020] c. Pass the decolorized liquid effluent obtained in step b into 300ml of macroporous exchange resin, and the adsorption rate on the column is 2BV / h; take the adsorption liquid for tryptophan content determination, and the ...

Embodiment 2

[0024] a. Heat and inactivate the tryptophan fermentation broth, remove bacteria by microfiltration membrane filtration, take 1L of the filtrate, the concentration of tryptophan is 22g / L, and put it into an anionic decolorization resin (special decolorization resin SQD-817) for decolorization , the column loading rate was 3BV / h, and then washed with 1L deionized water to collect 1.5L of the effluent; the tryptophan content of the effluent was measured to be 14.6g / L, and the loss rate of tryptophan was 1.5%;

[0025] b. Add 0.657 g of activated carbon (powdered, 3% of the total mass of tryptophan in the eluent) to the eluate obtained in step a, decolorize at a temperature of 45° C. for 15 minutes, and obtain the decolorized solution by suction filtration;

[0026] c. Pass the decolorized liquid effluent obtained in step b into 300ml macroporous exchange resin, and the adsorption rate on the column is 3BV / h. Get the adsorption liquid and carry out the tryptophan content determin...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
purityaaaaaaaaaa
transmittivityaaaaaaaaaa
purityaaaaaaaaaa
Login to View More

Abstract

The invention provides a production technology for extracting L-typtophan from a fermentation liquor. After microfiltration, most of proteins and pigments are removed by the aid of anion decolorization resin, further decolorization is performed by adding activated carbon, tryptophan is adsorbed by the aid of macroporous resin, most of impurities in the fermentation liquid are removed, elution is performed by adopting an ethanol solution, a tryptophan crystal is obtained after cooling crystallization of an eluant, a distillated ethanol solution obtained after a mother liquor is heated and evaporated rotarily can be recycled and continuously serve as the elution, and the mother liquor is further applied to the step of adsorption of the macroporous resin to have the tryptophan recovered; ultrafiltration operation of the fermentation liquior is omitted in the extraction technology, loss is avoided, product yield is increased, consumption of evaporation of concentrated vapor is saved, industrial water consumption and acid and alkali dosage are saved, and effluent disposal is reduced.

Description

technical field [0001] The invention relates to the field of L-tryptophan production, in particular to a production process for extracting L-tryptophan from fermentation broth. Background technique [0002] Tryptophan, as a common amino acid, has been widely recognized for its application value. As one of the 8 kinds of amino acids necessary for the human body, it has been widely used in the fields of feed, food and medicine. The production technology of tryptophan has gradually matured after years of research and development, and it is mainly obtained through microbial fermentation. Yeast powder and other components in the medium commonly used in tryptophan fermentation production today will make the color of the fermentation broth darker, and in the late stage of tryptophan fermentation, the bacteria will also produce some by-products with pigments, which will darken the color of the fermentation broth . There are also proteins produced by some bacteria in the fermentati...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C07D209/20
CPCC07D209/20
Inventor 徐庆阳陈宁刘子强谢希贤张成林李燕军范晓光马倩杨梦晨刘镇瑜户红通蔡萌萌
Owner TIANJIN UNIV OF SCI & TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com