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Method for combined detection of sulfate reducing bacteria and nitrate reducing bacteria in petroleum sewage

A joint detection and sulfate technology, applied in the direction of biochemical equipment and methods, microbial measurement/inspection, etc., can solve the problems of small scope of application, complicated operation, etc., and achieve the effect of easy operation, simple method, accurate detection and counting

Active Publication Date: 2017-09-05
陕西延长石油丰源石油助剂有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The method for joint detection of sulfate-reducing bacteria and nitrate-reducing bacteria in petroleum sewage of the present invention solves the limitations of the existing SRB and NRB bacteria counting detection technology, the problems of small application range, complicated operation, etc., and the method can be accurate, simple, Rapid SRB and NRB bacterial count test

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  • Method for combined detection of sulfate reducing bacteria and nitrate reducing bacteria in petroleum sewage
  • Method for combined detection of sulfate reducing bacteria and nitrate reducing bacteria in petroleum sewage
  • Method for combined detection of sulfate reducing bacteria and nitrate reducing bacteria in petroleum sewage

Examples

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Embodiment 1

[0031] This embodiment is a process of detecting sulfate-reducing bacteria and nitrate-reducing bacteria in the production water of the first-stage production separator in the production treatment process of an oilfield. The specific process is as follows:

[0032] After about 3 minutes after the water phase outlet of the on-site primary separator discharged the residual liquid in the pipeline, about 200 mL of water sample was collected in a sealed bottle, and sent back to the laboratory in time for storage at a low temperature of 4 °C.

[0033] Prepare the ferrous salt solution required for the counting and detection of sulfate-reducing bacteria, weigh 1.4000 g of the analytically pure ammonium ferrous sulfate (hexahydrate) reagent using a balance of 1 / 10,000, and carry out ultraviolet sterilization for 30 minutes under an ultra-clean workbench, and take 100 mL of sterile water sterilized under high temperature and high pressure at 120°C for 30 minutes and ferrous ammonium su...

Embodiment 2

[0047] This embodiment is a process of detecting sulfate-reducing bacteria and nitrate-reducing bacteria in a water injection sample of an oilfield water injection manifold. The specific process is as follows:

[0048] After draining the residual liquid in the pipeline for about 3 minutes at the sampling point of the on-site water injection manifold, collect about 100 mL of water sample in a sealed bottle, and send it back to the laboratory in time for storage at a low temperature of 5 °C.

[0049] Prepare the ferrous salt solution required for the counting and detection of sulfate-reducing bacteria, weigh 0.3500 g of the analytically pure ammonium ferrous sulfate (hexahydrate) reagent with a balance of 1 / 10,000, and carry out ultraviolet sterilization for 40 minutes under an ultra-clean workbench, and take Mix and dissolve 100 mL of sterile water sterilized under high temperature and high pressure at 125°C for 40 minutes and ferrous ammonium sulfate reagent sterilized by ultr...

Embodiment 3

[0063] This embodiment is a process of detecting sulfate-reducing bacteria and nitrate-reducing bacteria in the water samples of the production water buffer tank in the production and treatment process of an oil field. The specific process is as follows:

[0064] After the water phase outlet of the on-site production water buffer tank discharges the residual liquid in the pipeline for about 3 minutes, collect a water sample of about 200 mL in a sealed bottle and send it back to the laboratory in time for storage at a low temperature of 5 °C.

[0065] Prepare the ferrous salt solution required for the counting and detection of sulfate-reducing bacteria, weigh 3.500 g of the analytically pure ammonium ferrous sulfate (hexahydrate) reagent using a balance of 1 / 10,000, and carry out ultraviolet sterilization for 40 minutes under an ultra-clean workbench, and take Mix and dissolve 100 mL of sterile water sterilized under high temperature and high pressure at 120°C for 40 minutes an...

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Abstract

The invention belongs to the field of bacteria detection and relates to a method for combined detection of sulfate reducing bacteria and nitrate reducing bacteria in petroleum sewage. The method includes following steps: (1), performing concentration gradient dilution on a collected petroleum sewage sample; (2), putting the diluted petroleum sewage sample in a detection bottle for constant-temperature culture, and performing counting detection on sulfate reducing bacteria and nitrate reducing bacteria respectively, wherein culture liquid is composed of sodium lactate, yeast powder, sodium chloride, potassium dihydrogen phosphate / dipotassium hydrogen phosphate, ammonium chloride, potassium / sodium nitrate, magnesium sulfate, ascorbic acid, ethylene diamine tetraacetic acid and glutathione. The method solves the problems of limitation, narrow application range and complex operation of existing SRB and NRB bacteria counting detection technology and can realize SRB and NRB bacteria counting detection accurately, simply and quickly.

Description

technical field [0001] The invention belongs to the field of bacteria detection, and in particular relates to a method for joint detection of sulfate-reducing bacteria and nitrate-reducing bacteria in petroleum sewage. Background technique [0002] Sulfate-reducing bacteria (SRB) are microorganisms that use sulfate or other oxidized sulfides in sewage as electron acceptors to dissimilate organic substances. 4 2- reduced to H 2 S, and then change the pH of the water quality and the corrosion state of the metal, an important factor that significantly increases the corrosion rate of the metal, and brings many harmful effects such as corrosion and blockage to the oil pipeline. [0003] Nitrate-reducing bacteria (NRB) is an antagonistic bacteria that exists in petroleum sewage systems and has a competitive advantage. It is very similar to SRB in terms of living habits and growth environment, but it does not produce H 2 S and other harmful substances. Therefore, NRB can be use...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/06
CPCC12Q1/06
Inventor 于亮
Owner 陕西延长石油丰源石油助剂有限公司