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Doped graphene based cholesterol sensor and preparation method and application

A graphene and sensor technology, applied in the field of cholesterol sensor and preparation and application, can solve the problems of damage and high cost, and achieve the effects of strong anti-interference ability, low detection limit and stable performance

Inactive Publication Date: 2017-09-05
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there are many blood cholesterol detection methods reported, such as gas-liquid chromatography-mass spectrometry, temperature measurement, molecular luminescence, colorimetry, electrochemical methods, etc., but the selectivity of these detection methods mostly depends on It is destructive to enzymes or antibodies that specifically recognize cholesterol, and the cost is quite expensive
However, there is no report on the application of doped graphene material sensor in cholesterol detection.

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  • Doped graphene based cholesterol sensor and preparation method and application
  • Doped graphene based cholesterol sensor and preparation method and application
  • Doped graphene based cholesterol sensor and preparation method and application

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Embodiment 1

[0035] A preparation method of a graphene-doped cholesterol enzyme biosensor based on the present embodiment, the specific preparation steps are as follows:

[0036] (1) The glassy carbon electrode with a diameter of 3 mm is sequentially filled with Al with a diameter of 0.3 μm and 0.05 μm 2 o 3 The powder was polished to a mirror surface, rinsed with distilled water, and then ultrasonically cleaned in absolute ethanol and distilled water for 1 min, and then the glassy carbon electrode was placed in 10 mL of potassium ferricyanide solution (5 mmol / L K 3 Fe(CN) 6+0.2mol / L KCl) at 0-0.8V by scanning 6 cycles with cyclic voltammetry to activate the electrode, take it out, wash it with distilled water, and dry it at room temperature to obtain a pretreated glassy carbon electrode.

[0037] (2) Take 100mg of graphite oxide in a beaker, add 50mL of deionized water and ultrasonically strip it for 4h, then add 6.25mL of FeCl 3 ·6H 2 O solution and 235 μL of pyrrole monomer were put...

Embodiment 2

[0044] A preparation method of a graphene-doped cholesterol enzyme biosensor based on the present embodiment, the specific preparation steps are as follows:

[0045] (1) The glassy carbon electrode with a diameter of 3 mm is sequentially filled with Al with a diameter of 0.3 μm and 0.05 μm 2 o 3 The powder was polished to a mirror surface, rinsed with distilled water, and then ultrasonically cleaned in absolute ethanol and distilled water for 1 min, and then the glassy carbon electrode was placed in 10 mL of potassium ferricyanide solution (5 mmol / L K 3 Fe(CN) 6 +0.2mol / L KCl) at 0-0.8V by scanning 6 cycles with cyclic voltammetry to activate the electrode, take it out, wash it with distilled water, and dry it at room temperature to obtain a pretreated glassy carbon electrode.

[0046] (2) Take 100mg of graphite oxide in a beaker, add 50mL of deionized water and ultrasonically strip it for 4h, then add 6.25mL of FeCl 3 ·6H 2 O solution and 235 μL of pyrrole monomer were pu...

Embodiment 3

[0056] A preparation method of a graphene-doped cholesterol enzyme biosensor based on the present embodiment, the specific preparation steps are as follows:

[0057] (1) The glassy carbon electrode with a diameter of 3 mm is sequentially filled with Al with a diameter of 0.3 μm and 0.05 μm 2 o 3 The powder was polished to a mirror surface, rinsed with distilled water, and then ultrasonically cleaned in absolute ethanol and distilled water for 1 min, and then the glassy carbon electrode was placed in 10 mL of potassium ferricyanide solution (5 mmol / L K 3 Fe(CN) 6 +0.2mol / L KCl) at 0-0.8V by scanning 6 cycles with cyclic voltammetry to activate the electrode, take it out, wash it with distilled water, and dry it at room temperature to obtain a pretreated glassy carbon electrode.

[0058] (2) Take 100mg of graphite oxide in a beaker, add 50mL of deionized water and ultrasonically strip it for 4h, then add 6.25mL of FeCl 3 ·6H 2 O solution and 235 μL of pyrrole monomer were pu...

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Abstract

The invention belongs to the technical field of enzyme biosensors and discloses a doped graphene based cholesterol sensor and a preparation method and application. The sensor is composed of a reference electrode, a counter electrode and a modified working electrode, the modified working electrode is composed of a working electrode and a substance recognition film cured on the surface of the working electrode, and the substance recognition film is composed of a doped graphene composite material, Meldola's blue, cholesterol oxidase, horseradish peroxidase and perfluorosulfonated resin. The biosensor obtained for detection cholesterol can be used for detecting serum total cholesterol or cholesterol in food and has wide detection range and low detection limit, reaction is performed in a neutral environment at room temperature, and the sensor is stable in performance and has good application prospect.

Description

technical field [0001] The invention belongs to the technical field of enzyme biosensors, in particular to a cholesterol sensor based on doped graphene and its preparation and application. Background technique [0002] Cholesterol is an indispensable and important substance in the human body. It directly participates in the formation of cell membranes and is also a raw material for the synthesis of bile acids, vitamin D and steroid hormones. About 75% of the cholesterol in the human body is self-synthesized, and 25% is obtained from food intake. The cholesterol content in the human body must be maintained in a normal concentration range. If the cholesterol concentration is too high, it will cause diabetes, heart disease, and atherosclerosis. , hepatobiliary system diseases and other diseases; if it is too low, you need to check for serious illness or malnutrition, and it will increase the risk of depression, hemorrhagic stroke and other diseases. At present, there are many ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327G01N27/48
CPCG01N27/3275G01N27/48
Inventor 朴金花陈东霞张力姜建国
Owner SOUTH CHINA UNIV OF TECH
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