Method for efficiently and rapidly preparing and separating three kinds of ginsenoside isomers Rg6, Z type and E type F4

Abstract

The invention discloses a method for efficiently and rapidly preparing and separating three kinds of ginsenoside isomers Rg6, Z type and E type F4. The preparation method of a saponin mixture is as follows: ginsenoside Rg2 is used as a raw material, an acid-containing alcohol-water solution is used as a reaction system, a thermal dehydration reaction is carried out, and lead dioxide is used as a catalyst. A separation method of the saponin mixture is as follows: high-speed countercurrent chromatography is used for separation, and ethyl acetate-ethanol-water-acetic acid (volume ratio is 4:1:5:0.08) is used as a solvent system. The method for preparing a mixture of ginsenoside isomers Rg6, Z type and E type F4 has high yield, 90% or above of ginsenoside Rg2 is converted into Rg6, (Z)-F4 and (E)-F4, and utilization rate of raw materials is high; The method for separating the mixture of ginsenoside isomers Rg6, Z type and E type F4 by high speed countercurrent has high separation efficiency and good separation degree, three kinds of isomers can be effectively separated, and once separation is carried out in order to obtain three kinds of isomers, wherein each mass of the isomers is 100mg or above.

Description

technical field [0001] The invention belongs to the field of medicine preparation and relates to the separation and preparation of natural product isomers, in particular to an efficient and rapid method for preparing and separating three ginsenoside isomers Rg6, Z-type and E-type F4. Background technique [0002] The C20 position of dammarane-type ginsenosides mostly has a hydroxyl group, which is widely found in plants of the genus Panax genus, and its content is relatively high. After heat treatment, the C20 hydroxyl group of dammarane type saponins and their aglycones is prone to dehydration reaction, forming double bonds, and generating a series of C20 dehydroxylated dammarane type rare ginsenosides and their aglycones. [0003] CN102911238 A discloses the following reaction formula: [0004] [0005] But in fact, the dehydration products also include rare ginsenosides with D structure, whose chemical formula is as follows: [0006] [0007] Rare ginsenosides B, ...

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Problems solved by technology

[0013] Studies have shown that Rg6, Z-type and E-type F4 have different pharmacological effects, but due to the difficulty in obtaining standard products, they cannot be prepared in large quantities, which limits researchers to further study their activities.

Although CN102911238 A mentioned above discloses a method for preparing Rg6, F4, the method yield is low (less than 20%); Obtain three isomers (more than 100 mg each) to separate Rg6, Z-type and E-type F4

[0014] In short, it is difficult to obtain a large number of ginsenoside Rg6, Z-type and E-type F4 standard products

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