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Application of basic acid in the preparation of anti-influenza virus drugs

An influenza virus-rescuing technology of bis-acid, which is applied in the field of bis-rescue to achieve the effect of reducing lung index

Active Publication Date: 2020-02-21
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is no research report on the anti-influenza virus effect of basic acid

Method used

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  • Application of basic acid in the preparation of anti-influenza virus drugs
  • Application of basic acid in the preparation of anti-influenza virus drugs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] Example 1 Preparation of the medicinal extract

[0013] Rescue Bing medicinal materials appropriate amount, pulverize, pass through No. 1 sieve, add 8 times the amount of 70% (volume fraction) ethanol to the medicinal materials, extract under reflux for 2 times, 1 hour each time, filter, combine the extracts, put them in a rotary evaporator, and put them in a rotary evaporator. It was concentrated under reduced pressure at 80°C to a thick paste. Transfer the concentrated solution into a drying oven under reduced pressure, continue to dry and pulverize it at 80°C, and obtain the medicinal material extract of Salvation Bing.

Embodiment 2

[0014] Example 2 In vitro influenza virus inhibition experiment

[0015] 1. Experimental materials

[0016] 1.1 Test drug: salvage acid (extracted from salvage, purity > 98%).

[0017] 1.2 Control drugs: ribavirin, oleanolic acid, Jiubing medicinal material extract (see Example 1)

[0018] 1.3 Virus strain: mouse lung-adapted strain of influenza A H1N1 virus (FM / 1 / 47 strain). Chicken embryos were passaged, tested in BSL-3 (Biosafety Laboratory Level 3) laboratory, subpackaged, and stored at -80°C.

[0019] 1.4 Cell Model: Dog Kidney Cell MDCK Cell

[0020] The above medicines were fully dissolved in dimethyl sulfoxide (DMSO) and stored at -20°C for later use.

[0021] 2. Experimental method

[0022] 2.1 Determination of virus virulence

[0023] MDCK cells were pressed 5 x 10 4 96-well culture plates were seeded at a concentration of / mL, 100 μl per well, 37°C, 5% CO 2 Cultivated to form a confluent state of cell monolayers. 100 μl of the 10-fold serial dilution of the...

Embodiment 3

[0032] Example 3 In vivo influenza inhibition experiment

[0033] 1. Experimental materials

[0034] 1.1 Test drug: salvage acid (extracted from salvage, purity > 98%).

[0035] 1.2 Control drugs: Tamiflu, oleanolic acid, and the extract of Jiubing medicinal materials (see Example 1)

[0036] 1.3 Virus: mouse lung-adapted strain of influenza A H1N1 virus (FM / 1 / 47 strain). Chicken embryos were passaged, tested in BSL-3 (Biosafety Laboratory Level 3) laboratory, subpackaged, and stored at -80°C.

[0037] 2. Experimental animals

[0038] 80 BALB / c mice weighing 18g-22g were purchased from Guangdong Provincial Laboratory Animal Center.

[0039] 3. Experimental method

[0040] BALB / c mice were randomly divided into 8 groups, namely blank control group, model control group, Tamiflu control group, oleanolic acid group, Jiubing medicinal material extract group, and Jiubing acid high, medium and low. 3 dose groups, 10 mice in each group. Except for the blank control group, the a...

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Abstract

The present invention discloses applications of rotundic acid in preparation of anti-influenza virus drugs, further applications of rotundic acid in preparation of drugs for treatment of pneumonia caused by influenza viruses, wherein the influenza virus is preferably influenza A H1N1 virus. According to the present invention, the in vitro cell experiment results prove that the IC50 value of the rotundic acid on H1N1 influenza virus is 5.8 [mu]g / mL, and is comparable to the IC50 value of the positive drug ribavirin; and the mouse influenza model pharmacological experiment results prove that the rotundic acid can significantly reduce the lung index of influenza-infected mice, and shows a certain dose-effect characteristic on H1N1.

Description

technical field [0001] The invention discloses a new application of salvage acid, in particular to the application of salvage acid in the preparation of anti-influenza virus drugs. Background technique [0002] Influenza virus (influenza virus for short) can cause acute respiratory infection and pose a threat to human health. Among them, influenza A virus is easy to mutate due to its antigenicity, and the influenza A H1N1 influenza caused by it has the characteristics of wide prevalence, strong infectivity and high morbidity. At present, the response to influenza virus is mainly through vaccine and drug treatment. Among them, M2 ion channel inhibitors amantadine and neuraminidase inhibitors are commonly used clinical anti-influenza virus drugs in many countries, but these two types of anti-viral drugs are now resistant to varying degrees, and some certain toxic side effects. Therefore, it is very important and urgent to find new, high-efficiency and low-toxic anti-influen...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/56A61P31/16A61P11/00
CPCA61K31/56
Inventor 王永刚姚宏亮张伟建彭维李沛波苏薇薇
Owner SUN YAT SEN UNIV
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