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Aptamer modified core-shell type composite material and preparation method and application thereof

A nucleic acid aptamer and composite material technology, applied in the fields of nanotechnology and biomedicine, can solve the problems of small specific surface area, large environmental impact of SERS effect, inability to efficiently load drugs, etc., to ensure SERS imaging and excellent biocompatibility. And target recognition performance, improve the effect of drug loading rate

Active Publication Date: 2017-09-15
SUN YAT SEN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, gold nanoparticles are prone to agglomeration, and the SERS effect is greatly affected by the environment; and the specific surface area is small, so it cannot efficiently load drugs

Method used

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  • Aptamer modified core-shell type composite material and preparation method and application thereof
  • Aptamer modified core-shell type composite material and preparation method and application thereof
  • Aptamer modified core-shell type composite material and preparation method and application thereof

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preparation example Construction

[0034] The preparation method of the nucleic acid aptamer modified core-shell type composite material of the present invention comprises the following steps:

[0035] S1: Preparation of gold nanoparticles by reducing chloroauric acid with sodium citrate;

[0036] S2: adding a mercaptolation reagent to the solution of the gold nanoparticles prepared in step S1, stirring and reacting, and centrifuging to obtain mercapto-modified gold nanoparticles;

[0037]S3: Disperse the mercapto-modified gold nanoparticles prepared in step S2 in an alcoholic solvent, add copper acetate, centrifuge after ultrasonication, take the precipitate and disperse it in an alcoholic solvent, add trimesic acid, and perform coordination by ultrasonication Reaction, centrifugal separation to obtain the core-shell composite material;

[0038] S4: Disperse the core-shell composite material prepared in step S3 in Tris-HCl buffer, add a cross-linking agent, stir and react, and centrifuge to obtain a precipita...

Embodiment 1

[0045] Nucleic acid aptamer modified core-shell composite material and its preparation method and application

[0046] The nucleic acid aptamer modified core-shell composite material of the present invention includes a gold nanoparticle core, a metal-organic framework shell and a nucleic acid aptamer, the gold nanoparticle is coated in the metal-organic framework shell, and the nucleic acid adapter grafted onto the surface of the metal-organic framework.

[0047] see figure 1 , which is a schematic diagram of the synthesis of the nucleic acid aptamer modified core-shell composite material of the present invention, the preparation method of the nucleic acid aptamer modified core-shell composite material comprises the following steps:

[0048] Step S1: Prepare gold nanoparticles by reducing chloroauric acid with sodium citrate; specifically, take 200mL of chloroauric acid solution with a mass fraction of 0.01% and add it to a 500mL three-neck flask, heat to 145°C, and keep magn...

Embodiment 2

[0054] (1) Stability of surface Raman spectra of nucleic acid aptamer-modified core-shell composites

[0055] Use a PBS solution with a pH of 7.4 to prepare a PBS solution of a nucleic acid aptamer-modified core-shell composite material with a concentration of 5 mg / L, and mix the PBS solution of the composite material with NaCl solutions of different concentrations at a volume ratio of 8:2. The concentration of NaCl is 0.01, 0.05, 0.1, 0.5 mol / L in sequence. After mixing, all were stirred at 37° C. for 2 h, and then Raman spectra were collected. see image 3 , which is the Raman spectrum of the nucleic acid aptamer-modified core-shell composite material in different concentrations of NaCl solutions. It can be seen from the figure that with the increase of NaCl concentration, the Raman spectrum of the nucleic acid aptamer-modified core-shell composite material does not change significantly, indicating that the composite material has better SERS stability in salt solution . ...

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Abstract

The invention relates to an aptamer modified core-shell type composite material and a preparation method and application thereof. The aptamer modified core-shell type composite material comprises a gold nanoparticle core, a metal-organic framework shell and an aptamer, wherein gold nanoparticles are cladded in the metal-organic framework shell; the aptamer is grafted on the surface of the metal-organic framework shell; a metal-organic framework is a metal-organic framework made through coordination assembly on copper and trimesic acid; the aptamer is an anti-lung cancer cell A549 aptamer; and the base sequence of the anti-lung cancer cell A549 aptamer is 5'-NH2-(CH2)6-GGTTGCATGCCGTGGGGAGGGGGGTGGGTTTTATAGCGTACTCAG-3'. The aptamer modified core-shell type composite material provided by the invention has three functions of SERS (Surface Enhanced Raman Scattering) imaging, photothermal therapy and drug therapy at the same time, and can effectively realize coordinating functions of diagnosis and treatment.

Description

technical field [0001] The invention relates to the fields of nanotechnology and biomedicine, in particular to a nucleic acid aptamer-modified core-shell composite material and its preparation method and application. Background technique [0002] Nanomaterials have unique physical-chemical properties. In recent years, the diagnosis and treatment of diseases based on nanomaterials have attracted people's attention. The commonly used cancer treatment methods of nanomaterials are photothermal therapy and chemotherapy, and the synergistic application of hyperthermia and chemotherapy can effectively enhance the therapeutic effect. In order to achieve precise treatment, diagnosis and treatment are generally combined. Surface-enhanced Raman spectroscopy (SERS) has the advantages of high sensitivity and good photobleaching resistance, and has been successfully applied in cell imaging and cancer diagnosis. Gold nanoparticles have good biocompatibility, high SERS activity and photot...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K41/00A61K49/00A61K9/51A61K47/26A61K47/02A61K45/00A61P35/00
CPCA61K41/0052A61K45/00A61K49/0013A61K9/5115A61K9/5123
Inventor 李攻科贺锦灿胡玉玲
Owner SUN YAT SEN UNIV
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