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Disposable target plate for matrix-assisted laser desorption/ionization (MALDI) mass spectrometer

A matrix-assisted laser and chemical mass spectrometer technology, applied in the direction of instruments, scientific instruments, measuring devices, etc., can solve the problems of difficult cleaning, use, and difficult disposable consumables, so as to improve the sensitivity or signal strength of mass spectrometry analysis and avoid the cleaning process , the effect of improving the sensitivity

Pending Publication Date: 2017-09-15
CHUAN-MING (NINGBO) CHEM SCITECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, most target plates are made of stainless steel, which is repeatedly used, difficult to clean, and difficult to completely avoid cross-contamination
Especially for MALDI-MS target plates with surface modification features, their modifications are easily destroyed during repeated use
At the same time, the current commercialized target plate is expensive and difficult to use as a disposable consumable

Method used

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  • Disposable target plate for matrix-assisted laser desorption/ionization (MALDI) mass spectrometer
  • Disposable target plate for matrix-assisted laser desorption/ionization (MALDI) mass spectrometer
  • Disposable target plate for matrix-assisted laser desorption/ionization (MALDI) mass spectrometer

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Modify the surface of the disposable target plate of the present invention, use Teflon spray to modify the surface of the stainless steel thin layer to obtain a hydrophobic layer, and use the laser engraving method to obtain an array of hydrophilic points with a diameter of 0.5 microns, and the distance between the hydrophilic points about 7 mm. The top view of the prepared target plate is as follows figure 2 As shown, the conductive thin layer is above the hydrophobic layer 3, and the hydrophilic points 4 are evenly distributed on the hydrophobic layer. The sample to be tested is limited within the hydrophilic point 4.

[0037] Spot one microliter of bacteria (containing 10^5, 2000, or 100 bacterial cell copies) water-based suspension on the hydrophilic point, and after the solvent evaporates, add 1 microliter of matrix solution (2,5 dihydroxy Aqueous solution of benzoic acid, 10mg / ml), after the solvent evaporates, a dry sample to be tested is formed.

[0038] As ...

Embodiment 2

[0043] Modification is carried out on the disposable target plate surface of the present invention, titanium oxide nanopowder (1g, P25, 21 nm particle size, Sigma-Aldrich) were heated at 300°C for 2 hours and separated in a mortar for 3 hours. During the separation, 1 ml of 10% aqueous acetic acid was added dropwise. The isolated nanoparticles were suspended in aqueous ethanol (89%, v / v) to a concentration of 100 mg / ml, and then sonicated for 1 hour. The resulting TiO2 suspension was diluted 25-fold in deionized water. The final TiO2 suspension was deposited on sample spots (2.5 microliters per spot) in a thin layer of stainless steel. After drying at room temperature, the TiO2 was sintered at 400 °C for 1 h. get Figure 4 The surface titanium oxide modified target plate in . The lowermost conductive support 1, and above the conductive support 1 is a conductive thin layer 2, and on the conductive thin layer 2, nanomaterial modification points 5, which are titanium oxide ...

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Abstract

The invention provides a disposable target plate for a MALDI mass spectrometer. The disposable target plate comprises a conductive support and a conductive thin layer, wherein the conductive support is repeatedly usable while the conductive thin layer is disposable; the conductive support and the conductive thin layer are detachably connected; and the conductive thin layer covers the upper surface of the conductive support and closely contacts with the conductive support. The invention provides the disposable and cheap MALDI target plate to prevent cross contamination and cleaning in repeated usage; and surface modification can be carried out on the target plate so as to improve sensitivity or signal intensity.

Description

technical field [0001] The invention belongs to detection instruments, and relates to a disposable target plate used for a matrix-assisted laser desorption ionization mass spectrometer. Background technique [0002] Matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS) is widely used in the analysis of biological samples such as tissue sections, cells, microorganisms, proteins, polypeptides, and nucleic acids. In recent years, MALDI-MS has been used as a medical device for microbial identification and nucleic acid single point mutation identification. [0003] Target plates are an important part of MALDI-MS. So far, most target plates are made of stainless steel, which are reused repeatedly, difficult to clean, and difficult to completely avoid cross-contamination. Especially for MALDI-MS target plates with surface modification features, their modifications are easily destroyed during repeated use. At the same time, the current commercialized target pla...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/64
CPCG01N27/64
Inventor 余绍宁
Owner CHUAN-MING (NINGBO) CHEM SCITECH CO LTD