Fibrous membrane device capable of adsorbing-desorbing protein and based on 3D printing PLA material and preparation method thereof

A 3D printing and fiber membrane technology, applied in the field of protein separation and purification, can solve the problems of increasing process flow, increasing processing costs, limiting the expansion of separation and purification application fields, etc., to achieve the goal of ensuring separation efficiency, improving adsorption and improving recyclability Effect

Active Publication Date: 2017-09-22
AEROSPACE INST OF ADVANCED MATERIALS & PROCESSING TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Cellulose diacetate is a cellulose derivative obtained by esterifying cellulose under the action of a catalyst, and then hydrolyzing the product to reduce its acetyl content; the molecular structure of cellulose acetate itself has no specific adsorption on proteins

Method used

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  • Fibrous membrane device capable of adsorbing-desorbing protein and based on 3D printing PLA material and preparation method thereof
  • Fibrous membrane device capable of adsorbing-desorbing protein and based on 3D printing PLA material and preparation method thereof
  • Fibrous membrane device capable of adsorbing-desorbing protein and based on 3D printing PLA material and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] S11. Add 8g of bamboo pulp to the oxidation system of TEMPO (0.04g), NaBr (0.4g) and NaClO (10g), adjust the pH to 10.2 by NaOH, then react for 6.5h, and obtain the product by washing, filtering, and ultrasonic centrifugation Aqueous suspension of nanocellulose with a solid content of 0.2%.

[0046] 8.2 g of dimethyl sulfoxide was slowly added dropwise into the aqueous suspension, and then vacuumized and rotary evaporated at 80° C. for 4 hours to obtain a nanocellulose dimethyl sulfoxide suspension.

[0047] S12. Dissolve 1.64g of cellulose diacetate (the degree of substitution of acetyl group is 2.5, the molecular weight is 39000) in 8.03g of nanocellulose dimethyl sulfoxide suspension, until the cellulose diacetate is completely dissolved and the solution is transparent , and then 0.2g dimethyl sulfoxide and 6.1g trichloromethane were added dropwise into the solution, and then magnetically stirred at room temperature for 4 hours, then the stirring was stopped, left st...

Embodiment 2

[0052] S21. Add 8g of bamboo pulp to the oxidation system of TEMPO (0.04g), NaBr (0.4g) and NaClO (10g), adjust the pH to 10.5 by NaOH, then react for 6.5h, and the product is obtained by washing, filtering, and ultrasonic centrifugation. Aqueous suspension of nanocellulose with a content of 0.2%.

[0053] 5.25 g of dimethyl sulfoxide was slowly added dropwise into the aqueous suspension, followed by rotary evaporation at 80° C. for 4 hours under vacuum to obtain a nanocellulose dimethyl sulfoxide suspension.

[0054] S22. Dissolve 2.1 g of cellulose diacetate (with an acetyl substitution degree of 2.3 and a molecular weight of 60,000) in the nanocellulose dimethyl sulfoxide suspension obtained in S21, and wait until the cellulose diacetate is completely dissolved and the solution becomes transparent , then add 4.65g dimethyl sulfoxide and 9.06g trichloromethane dropwise into the solution, then magnetically stir at room temperature for 8h, stop stirring, stand for defoaming, a...

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Abstract

The invention relates to a fibrous membrane device capable of adsorbing-desorbing protein and based on a 3D printing PLA material and a preparation method thereof. A nanocellulose/cellulose diacetate composite spinning solution is prepared into nanocellulose/cellulose diacetate composite nano-fibers through electrostatic spinning, the nanocellulose/cellulose diacetate composite nano-fibers are accumulated on a PLA supporting body prepared through the 3D printing technology, and a nanocellulose/cellulose diacetate composite nano-fiber membrane, namely the fibrous membrane device is formed. The formula of the spinning solution is optimized, the PLA supporting body is introduced to serve as a base body support of the fibrous membrane device, effective support is provided for the device under the high-pressure-drop and high-flux separation condition, the reusability of the fibrous membrane can be improved, and the separation efficiency of protein is guaranteed. The membrane device has the high adsorption amount on bovine serum albumin, after the adsorption-desorption process is circulated five times, the desorption rate can still be kept at 80% or above, and the device can be widely applied to protein purification and separation.

Description

technical field [0001] The invention relates to the technical field of separation and purification of proteins, in particular to a fiber membrane device capable of adsorbing and desorbing proteins based on 3D printing PLA materials and a preparation method thereof. Background technique [0002] In recent years, 3D printing technology, as an emerging material processing and molding technology, has been called "manufacturing technology with industrial revolution significance". 3D printing technology is mainly based on a three-dimensional mathematical model, using materials of different shapes (powder, droplet, and line) to form three-dimensional entities with different shapes and structures through point-by-point or layer-by-layer integration and accumulation methods. Because of the characteristics of flexible manufacturing, 3D printing technology can freely print products with unique shapes and complex internal structures. This technology can be applied to the preparation of ...

Claims

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Application Information

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IPC IPC(8): D04H1/4382D04H1/728D01D5/00B29C64/118B29C64/393B33Y50/02B33Y80/00B01J20/24B01J20/28B01J20/30
CPCB01J20/24B01J20/28038B01J2220/44B01J2220/4825B33Y50/02B33Y80/00D01D5/003D01D5/0084D04H1/4382D04H1/728
Inventor 兰天裴雨辰杨洁颖李南孟庆杰梁垠徐亮杨帆
Owner AEROSPACE INST OF ADVANCED MATERIALS & PROCESSING TECH
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