Production method of enrichment gamma-aminobutyric acid in barley malt
A technology of aminobutyric acid and production methods, applied in horticultural methods, botanical equipment and methods, horticulture, etc., to achieve the effects of improving nutritional value, increasing economic value, and high-efficiency enrichment
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Embodiment 1
[0019] Select barley grains with full grains and no pests and diseases, soak in 1.5% sodium hypochlorite solution for 10 min, then wash with distilled water until neutral pH; put in water at a solid-liquid ratio of 0.1 g / mL, soak at 25 °C for 35 h, During the soaking period, the air was continuously ventilated at a rate of 3.0 L / min; then the barley was germinated at 25°C for 60 hours, and the mixed aqueous solution was sprayed once every 12 hours during the germination period. The mixed aqueous solution contained 25 mmol / L sodium chloride and 3 Mmol / L calcium chloride, the ratio of spraying amount to barley kernel mass was 1.5mL / g, and barley malt was prepared, and its γ-aminobutyric acid content was 0.1 mg / g DW.
Embodiment 2
[0021] Select barley grains with full grains and no pests and diseases, soak them in 1.5% sodium hypochlorite solution for 10 min, and then wash them with distilled water until the pH is neutral; put them in water at a solid-liquid ratio of 0.15 g / mL, and soak them at 27°C for 30 h. During the soaking period, the air was continuously ventilated at a rate of 4.0 L / min; then the barley was germinated at 27°C for 55 hours, and the mixed aqueous solution was sprayed once every 12 hours during the germination period. The mixed aqueous solution contained 35 mmol / L sodium chloride and 4 Mmol / L calcium chloride, the ratio of spraying amount to barley kernel mass was 2.0 mL / g, and barley malt was prepared, and its γ-aminobutyric acid content was 0.2 mg / g DW.
Embodiment 3
[0023] Select barley grains with full grains and no pests and diseases, soak in 1.5% sodium hypochlorite solution for 10 min, and then wash with distilled water until neutral pH; put them in water at a solid-liquid ratio of 0.20 g / mL, soak at 30 °C for 25 h, During the soaking period, the air was continuously ventilated at a rate of 4.0 L / min; then the barley was germinated at 28°C for 50 hours, and the mixed aqueous solution was sprayed once every 12 hours during the germination period. The mixed aqueous solution contained 40 mmol / L sodium chloride and 4 Mmol / L calcium chloride, the ratio of spraying amount to barley kernel mass was 2.5mL / g, and barley malt was prepared, and its γ-aminobutyric acid content was 0.4 mg / g DW.
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