Tea seed saponin as well as preparation method and application thereof

A technology of tea seed saponin and tea seed, which is applied in the field of tea seed saponin and its preparation, can solve the problems of tea saponin such as poor color, complex process, and low purity, and achieve low solvent consumption, high yield purity, and selectivity high effect

Inactive Publication Date: 2017-09-29
GUANGZHOU UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the extraction methods of tea saponin have the disadvantages of complex process, time-consuming, low yield, etc., and the tea saponin has poor color and low purity, which cannot meet the requirements of the industry.

Method used

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  • Tea seed saponin as well as preparation method and application thereof
  • Tea seed saponin as well as preparation method and application thereof
  • Tea seed saponin as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] A preparation method of tea seed saponin, comprising the steps of:

[0050] (1) Tea seed cake is pulverized to obtain tea seed powder;

[0051] (2) Mix the tea seed powder prepared in step (1) with an alkaline ethanol solution with a pH of 9.07 and an ethanol volume fraction of 73.35% according to a solid-to-liquid ratio of 1:9. Under microwave-assisted extraction for 8 minutes, the extract was concentrated to obtain crude tea seed saponin;

[0052] (3) Weigh 10.0g of the crude tea seed saponin prepared in step (2), dissolve it in 200mL of hot water at 60°C, add active dry yeast to ferment for 6h, centrifuge, and take the supernatant; add Calcium oxide (16% of the mass of the supernatant), reacted in a constant temperature water bath at 30°C for 2 hours, centrifuged, discarded the supernatant, and obtained a precipitate;

[0053] (4) Mix the precipitate obtained in step (3), ammonium bicarbonate (2.5 times the mass of calcium oxide) and water, release in a constant te...

Embodiment 2

[0058] A preparation method of tea seed saponin, comprising the steps of:

[0059] (1) Tea seed cake is pulverized to obtain tea seed powder;

[0060] (2) The tea seed powder prepared in step (1) is mixed with an alkaline ethanol solution having a pH of 8 and an ethanol volume fraction of 60% according to a solid-to-liquid ratio of 1:10, and the temperature is 50°C and the microwave power is 650W. Under microwave-assisted extraction for 7 minutes, the extract was concentrated to obtain crude tea seed saponin;

[0061] (3) Weigh 10.0g of the crude tea seed saponin prepared in step (2), dissolve it in 200mL of hot water at 60°C, add active dry yeast to ferment for 6h, centrifuge, and take the supernatant; add Calcium oxide (16% of the mass of the supernatant) was reacted in a constant temperature water bath at 30° C. for 2 hours. Centrifuge, discard the supernatant, and obtain the precipitate;

[0062] (4) Mix the precipitate obtained in step (3), ammonium bicarbonate (2.5 ti...

Embodiment 3

[0066] A preparation method of tea seed saponin, comprising the steps of:

[0067] (1) Tea seed cake is pulverized to obtain tea seed powder;

[0068] (2) The tea seed powder prepared in step (1) is mixed with an alkaline ethanol solution having a pH of 9.5 and an ethanol volume fraction of 75% according to a solid-to-liquid ratio of 1:12, and the temperature is 70°C and the microwave power is 600W. Under microwave-assisted extraction for 10 minutes, the extract was concentrated to obtain crude tea seed saponin;

[0069] (3) Weigh 10.0g of the crude tea seed saponin prepared in step (2), dissolve it in 200mL of hot water at 60°C, add active dry yeast to ferment for 6h, centrifuge, and take the supernatant; add Calcium oxide (16% of the mass of the supernatant), reacted in a constant temperature water bath at 30°C for 2 hours, centrifuged, discarded the supernatant, and obtained a precipitate;

[0070] (4) Mix the precipitate obtained in step (3), ammonium bicarbonate (2.5 ti...

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Abstract

The invention belongs to the field of plant extracting and specifically relates to tea seed saponin as well as a preparation method and application thereof. According to the tea seed saponin disclosed by the invention, tea seed cake which is hardly utilized is utilized as a raw material, and a microwave-assisted alkalescent ethanol method is utilized to extract the tea seed saponin and perform further flocculation and purification to obtain the pure tea seed saponin. A critical micelle concentration of the prepared pure tea seed saponin is 3.9*10<-5> mol/L, the surface tension of the prepared pure tea seed saponin is 34.8mN/m under the concentration, and the tea seed saponin has relatively good solubilization property, rewetting property and emulsifying property and good foaminess and foam stability. The preparation method has the advantages of small extracting solvent consumption, high efficiency, time saving, high selectivity and high yield and purity. In addition, according to the tea seed saponin disclosed by the invention, biological active ingredients in the tea seeds are fully utilized, comprehensive utilization and maximum benefit of tea seed resources are achieved, and environmental pollution is reduced.

Description

technical field [0001] The invention belongs to the field of plant extraction, and in particular relates to a tea seed saponin and its preparation method and application. Background technique [0002] In recent years, due to its low toxicity and good biodegradability, natural surfactants have attracted the attention of researchers at home and abroad, and have gradually been widely used in various industries as substitutes for other types of surfactants. Tea saponin is a non-ionic natural surfactant with excellent performance. It has good functions of foaming, decontamination, emulsification, dispersion, penetration, wetting and foam stabilization. Itching and other pharmacological effects, can be widely used in daily chemical, textile, pesticide and other industries. For a long time, researchers at home and abroad have made comprehensive and in-depth research on the method and technology of extracting tea saponin from camellia oleifera seed dregs, but there has not been any...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01F17/56C09K23/56
CPCC09K23/00
Inventor 毛桃嫣郑成李小然单烨琪邹敏婷
Owner GUANGZHOU UNIVERSITY
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