A method of using rice bran meal to co-produce rice bran protein and 4-vinylguaiacol
A technology of rice bran protein and guaiacol, which is applied in the directions of microorganism-based methods, peptide preparation methods, chemical instruments and methods, etc., can solve the problems of high energy consumption and low utilization rate of rice bran, and achieves recycling and transformation. The effect of high rate and low production cost
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Embodiment 1
[0033] Example 1 Preparation of immobilized cell microspheres by sodium alginate embedding method
[0034]Bacillus DLF-15161 was inoculated into the liquid medium, cultured at 180rpm / min, 37°C until the stationary phase, and then the bacterial liquid was centrifuged at 9000r / min for 10min to obtain the bacterial sludge; a certain quality of the bacterial sludge and sodium alginate solution Mix to make the mass concentration of the sludge reach 5g / 100mL, use a syringe to evenly drop the mixture to the CaCl 2 solution, cross-linked at 4°C for 16 hours to obtain microspheres. Among them, the concentration of sodium alginate is 3g / 100mL, CaCl 2 Solution concentration 3g / 100mL.
[0035] The liquid medium composition is: glucose 5.0g / L, yeast extract 2.0g / L, KH 2 PO 4 1g / L,Na 2 HPO 4 12H 2 O 4g / L, CaCl 2 0.05g / L, MgSO 4 ·7H 2 O 0.2g / L, NaCl 0.2g / L.
Embodiment 2
[0036] Example 2 Utilize rice bran meal to co-produce rice bran protein and 4-vinylguaiacol
[0037] Utilize weak base (pH8.5) to soak the defatted rice bran according to the ratio of material to liquid 2g:10mL, put it into an ultrasonic cell breaker and ultrasonically 10min under 60W power, then use 0.1M NaOH or HCl seasoning solution pH value, Keep it at 8.5; carry out alkaline extraction of the treated feed liquid at 40°C for 1 hour (pH 8.5), centrifuge at 4500 rpm / min for 20 minutes to obtain the residue and supernatant, wash the residue once and centrifuge, and combine the supernatant; The supernatant was adjusted to a pH value of 4.5 for acid precipitation, centrifuged at 4500 r / min for 20 min, and the protein precipitate was collected and dried to obtain crude rice bran protein, with a protein extraction rate of 51.7%. Mix the rice bran residue and waste liquid produced by extracting protein according to the ratio of material to liquid 4g:20mL, and carry out alkaline hy...
Embodiment 3
[0038] Example 3 Co-production of rice bran protein and 4-vinylguaiacol by using rice bran meal
[0039] Utilize weak base (pH9.5) to soak the defatted rice bran according to the material-to-liquid ratio of 2g:30mL, put it into an ultrasonic cell breaker, and ultrasonicate it for 2min at a power of 100W, then use 0.1M concentration of NaOH or HCl to adjust the pH value of the liquid, Keep it at 9.5; carry out alkaline extraction of the treated feed liquid at 45°C for 1 hour (pH9.5), centrifuge at 4500rpm / min for 20 minutes to obtain the residue and supernatant, wash the residue once and centrifuge, and combine the supernatant; The supernatant was adjusted to a pH value of 4.5 for acid precipitation, centrifuged at 4500 r / min for 20 min, and the protein precipitate was collected and dried to recover crude rice bran protein, with a protein extraction rate of 54.6%. The rice bran residue and waste liquid produced by extracting protein were mixed according to the ratio of material...
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