Normal-temperature preservation diluent for seminal fluid of Guanzhong black pig

Abstract

The invention discloses a normal-temperature preservation diluent for seminal fluid of a Guanzhong black pig. The diluent mainly contains the following components: glucose, sodium citrate, ethylenediamine tetraacetic acid, sodium hydrogen carbonate, potassium chloride, kojic acid and epsilon-polylysine. By utilizing the normal-temperature preservation diluent, the seminal fluid of the Guanzhong black pig can be preserved at room temperature for 1-5 days and can meet the artificial insemination requirement. Kojic acid added into the diluent has good antioxidant and protective effects to semens, meanwhile, the use amount of antibiotics during pig-raising production is reduced, and the problem of the drug resistance of bacteria can be effectively alleviated, so that the problem of the preservation of the seminal fluid of the Guanzhong black pig in an artificial insemination technique adopted in a large-scale cultivation process is solved, the feeding of boars is reduced, and the utilization ratio of excellent boar semens is increased. A preparation method of the diluents is simple in operation, low in production cost and convenient for large-scale popularization and application.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a diluent formula for storing semen at room temperature for deep insemination of Guanzhong black pigs. Background technique [0002] Guanzhong black pig is the first batch of geographical indication products of the Ministry of Agriculture. It is named after it is produced and widely distributed in the Guanzhong Plain of Shaanxi. Guanzhong black pig is a meat-fat dual-purpose breed bred by crossbreeding Berkshire pig, Gansu Jingchuan Bamei pig and Sichuan Neijiang pig. It was identified in the early 1980s. It has high fecundity, good meat quality, rough feeding resistance, Excellent germplasm characteristics such as strong stress resistance. [0003] In the process of promoting the large-scale breeding of Guanzhong black pigs, the use of deep insemination technology can improve the use efficiency of the semen of high-quality boars, reduce the amount of boars raised, reduce production...

AI Technical Summary

Problems solved by technology

[0005] 1) At present, the dilution powders for room temperature storage of pig semen widely used in the market are mainly foreign brands, and there are few domestic self-developed products, and these dilution powders are not necessarily suitable for the dilution storage of Guanzhong black pig semen

[0006] 2) Antibiotics are mainly added to the commercial dilution powder for sterilization and antibacterial. However, in recent years, the problems of antibiotic residues and bacterial drug resistance have become increasingly prominent and need to be resolved.

[0007] 3) Sperm are prone to oxidative stress during storage at room temperature, which affects the quality of semen, thereby reducing the reproductive performance of sows

Images