Preparation method for low-molecular weight ascophyllum mackaii glycan and application

A technology of alcophyllan and low molecular weight, which is applied in the field of low molecular weight seaweed polysaccharide preparation, can solve the problems of shedding of active groups, loss of activity, change of polysaccharide three-dimensional structure, etc., and achieves the effect of mild reaction conditions and weak destructive effect.

Active Publication Date: 2017-10-10
JIMEI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although these methods can reduce the molecular weight of algal polysaccharides, the chemical reaction is strong. As a result, the three-dimensional structure of polysaccharides is changed, and at the same time, some active groups are caused to fall off, which ultimately reduces the biological activity of the product or even completely loses its activity.

Method used

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  • Preparation method for low-molecular weight ascophyllum mackaii glycan and application
  • Preparation method for low-molecular weight ascophyllum mackaii glycan and application
  • Preparation method for low-molecular weight ascophyllum mackaii glycan and application

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Embodiment 1

[0025] The invention discloses a preparation method of low molecular weight ascophyllan, comprising the following steps:

[0026] Step 1, preparation of Ascophyllum nodosum powder:

[0027] Washing, drying, and pulverizing the Ascophyllum nodosum, passing through a 60-mesh standard sieve to obtain the Ascophyllum nodosum powder;

[0028] Step 2, preparation of ascophyllan dry powder:

[0029] Weigh 30g of Ascophyllum nodosum powder, add deionized water according to the weight volume ratio of 1:30g / mL, extract at 100°C, stir continuously for 2h during the extraction process, centrifuge at 5000×g for 20min after cooling, take the supernatant, add 3M HCL to make the final concentration pH 1.3, centrifuge to remove the alginic acid precipitate, collect the supernatant, add absolute ethanol to make the final concentration 50% by volume, centrifuge overnight, take the precipitate, dialyze and freeze-dry, and obtain the foam Phyllophyllan dry powder;

[0030] Step 3. Enzymatic hyd...

Embodiment 2

[0034] Embodiment 2 single factor enzymatic hydrolysis experiment

[0035] 2.1 The effect of temperature on the degradation of ascophyllan by cellulase

[0036] In Step 3 of Example 1, the effects of different temperatures of 45°C, 50°C, 55°C, 60°C, and 65°C on the degradation of Ascophyllan by cellulase were investigated, such as figure 1 As shown, at 55°C, the reducing sugar concentration is the highest, that is, the enzymatic hydrolysis efficiency is the highest.

[0037] 2.2 The effect of enzyme concentration on the degradation of ascophyllan by cellulase

[0038] In Step 3 of Example 1, the influence of different enzyme concentrations 3U / ml, 4U / ml, 5U / ml, 6U / ml, and 7U / ml on the degradation of Ascophyllan by cellulase was investigated, such as figure 2 As shown, when the enzyme concentration is 5 U / ml, the reducing sugar concentration is the highest, that is, the enzymatic hydrolysis efficiency is the highest.

[0039] 2.3 The effect of pH on the degradation of ascoph...

Embodiment 3

[0042] The 0.5g ascophyllan dry powder prepared in step 2 of Example 1 was degraded by cellulase, alginate lyase, and xylanase to obtain low molecular weight ascophyllan fragments, respectively, and the oligomeric polysaccharides containing the different molecular fragments were respectively The ascophyllan aqueous solution adopts a small-scale ultrafiltration membrane separation device to separate the oligomeric ascophyllan of different molecular weights, and the peristaltic pump transports the oligomeric ascophyllan aqueous solution of the different molecular fragments to a molecular cut-off of 5.3L / min. 10kDa hollow modified polyvinyl chloride membrane for separation, pressure, when the solution volume is concentrated to 1 / 6, stop the ultrafiltration, collect the solution with a molecular cut-off of 10kDa, and when it is concentrated to 1 / 6 by rotary evaporation, stop the concentration, and Collect the concentrated solution to obtain the oligomeric Ascophyllan component with...

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Abstract

The invention discloses a preparation method for a low-molecular weight ascophyllum mackaii glycan and application. In the method, cellulase is adopted to hydrolyze ascophyllum mackaii glycan, so that an aqueous ascophyllum mackaii glycan solution with different molecular weights of segments is obtained, and the low-molecular weight ascophyllum mackaii glycan is then obtained by ultrafiltration membrane separation. The reaction conditions of the production process of preparing the low-molecular weight ascophyllum mackaii glycan by the enzyme method disclosed by the invention are mild, the controllability is high, and the prepared low-molecular weight glycan segment has good immunity-inducing activity. The prepared low-molecular weight ascophyllum mackaii glycan is a safe, green product when applied to the regulation of body immunity, and can be used in the development of functional food and medicine materials.

Description

technical field [0001] The invention relates to the technical field of preparation of low-molecular-weight seaweed polysaccharide, in particular to a preparation method and application of low-molecular-weight ascophyllan. Background technique [0002] Ascophyllum nodosum, also known as Chlamydomonas, is a large marine brown algae that mainly grows and reproduces along the coast of the North Atlantic Ocean. Similar to other brown algae such as kelp. Ascophyllum nodosum is rich in alginate acid (accounting for about 79% of the total sugar contained in algae), and is one of the main raw materials for industrial production of alginate, iodine and mannitol. In addition to alginate, Ascophyllum nodosum also contains ascophyllan (ascophyllan) and 8% fucoidan (fucoidan), which account for about 13% of the total sugar of the algal body. In my country, Ascophyllum nodosum is mainly used as a raw material for extracting alginate in industry; it is used to make fertilizers and feeds i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61K31/715A61P37/04
CPCA61K31/715C08B37/0003C08B37/0063
Inventor 姜泽东鲍青云倪辉朱艳冰肖安风黄高凌杜希萍陈艳红杨远帆李利君蔡慧农
Owner JIMEI UNIV
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