An Improved Special Staining Diagnosis Solution

Abstract

The invention provides a modified specific stain diagnostic solution and a preparation method thereof and aims to provide a detection reagent for cervical cancer screening with high detection sensitivity and reliable detection result. In a detection process, an acetic acid whitening reaction zone is formed on a diseased region under the effect of the acetic acid in a staining solution, so that the biopsy under colposcope can be performed in the acetic acid whitening reaction zone. The method utilizes methylene blue developing and acetic acid whitening reaction dual-positioning and indication to increase the detection rate of cervix uteri / vagina / vulva precancerous lesion. The stain solution provided by the invention is high in stability in the process of contacting with air, can avoid the false positive result caused by quick oxidization and can fully guarantee the accuracy of the detection result. The modified specific stain diagnostic solution is prepared from the following components by weight percent: 30%-70% of medical propylene glycol, 3%-7% of glacial acetic acid, 0.1%-5% of vitamin C, 0.05%-5% of folic acid, 0.05%-5% of sodium acetate, 0.03%-0.5% of methylene blue, 1%-10% of fructose, 1%-20% of glucose, 0.1%-0.5% of sucrose fatty acid ester and purified water for keeping constant volume.

Description

technical field [0001] The invention relates to a detection reagent, specifically, it belongs to special staining and enzyme histochemical staining diagnosis (solution), and is a living cell staining agent; the invention also relates to the composition and preparation method of the detection reagent. Background technique [0002] At present, cervical cancer screening relies heavily on multiple laboratory tests, such as: liquid-based cytology test (TCT), HPV genotyping test, colposcopy, etc. Before the above tests, doctors have a basis for the patient's suspected diagnosis For unclear problems, it takes a long time to issue reports at the same time, the cost of inspection is high, and patients are easy to lose. The methods used for cell-specific staining of epithelial tissue mainly include acetic acid albinism reaction, iodine test, and methylene blue staining. Special staining liquid has a long history of external diagnosis of diseases. It is an invasive in vitro staining d...

AI Technical Summary

Problems solved by technology

[0006] At present, most of the special dyes in the market use dimethyl sulfoxide (DMSO) as the main solvent, but studies have shown that DMSO has certain toxic effects, and interacts with protein hydrophobic groups, leading to protein denaturation, vascular toxicity and liver and kidney damage. Toxicity, it can burn the skin and make the skin feel tingling, just like the rash and blisters seen, because this type of dye needs to be applied to human tissue, so dimethyl sulfoxide has a safety risk

[0007] At present, such special dyes in the market have a strong oxidation effect, so the stability is difficult to solve. In clinical use, it needs to be prepared and used immediately. Its stability is only ten minutes, which is very inconvenient, and it is not convenient for large-scale screening and large outpatient visits. clinical examination

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