A kind of preparation method of superporous hydrogel

A technology of porous hydrogel and ultra-pure water, which is applied in the field of preparation of new ultra-porous hydrogel, can solve the problems of easy water loss closure, unstable pore structure, fast degradation rate, etc., to achieve enhanced molecular cross-linking reaction, Good biocompatibility, the effect of promoting the formation of pores

Active Publication Date: 2019-11-19
NORTHWEST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Due to the introduction of porous structure, it often directly leads to the obvious decline of the mechanical properties of the gel.
In addition, most of the current porous hydrogels generally have many defects, such as low porosity or poor pore connectivity; or unstable pore structure, easy to lose water and close; or poor biocompatibility; or degradation rate Too fast; or the function is relatively single, etc.

Method used

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  • A kind of preparation method of superporous hydrogel
  • A kind of preparation method of superporous hydrogel
  • A kind of preparation method of superporous hydrogel

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1 Preparation of porous hydrogel

[0035] (1) Dissolve the water-soluble protein HLC in 10 mL of distilled water to obtain an HLC solution with a concentration of 100 mg / mL, and add the cross-linking agent β-diimine zinc complex and 1, 2, 7, 8 with a concentration of 1%. -Diepoxyoctane each 1 mL, then add 10mg / mL KCl2mL, mix well, and adjust the pH of the solution to 4.5 with dilute hydrochloric acid;

[0036] (2) Dispense the mixed solution in (1) into a mold, keep it at 50°C for 2h, then transfer it to a high-pressure steam sterilizer and keep it at 121°C for 20min to obtain the initial gel;

[0037] (3) Soak and wash the initial gel in distilled water for 5 days, and change the washing water every 6 hours to remove salt and residual crosslinking agent; transfer the washed initial gel to a high-pressure steam sterilizer and keep at 121°C for 2 h, then soak and wash again with distilled water for 2 days, control the total amount of residual crosslinker to be less tha...

Embodiment 2

[0040] Example 2 Preparation of porous hydrogel

[0041] (1) Dissolve the water-soluble protein HSA in 10 mL of distilled water to obtain a HSA solution with a concentration of 100 mg / mL, and add the cross-linking agent β-diimine zinc complex and 1, 2, 7, 8 with a concentration of 2%. -Diepoxy octane each 1 mL, then add 10mg / mL Na 2 SO 4 2mL of the solution, mix well, and adjust the pH of the solution to 3.5 with dilute hydrochloric acid;

[0042] (2) Dispense the mixed solution in step (1) into a mold, keep it at 50°C for 2h, then transfer it to a high-pressure steam sterilizer and keep it at 110°C for 20min to obtain the initial gel;

[0043] (3) Step (2) Soak and wash with distilled water for 2 days to remove the residual cross-linking agent, and control the total amount of residual cross-linking agent to be less than 2 μg / g to obtain a porous wet gel sample;

[0044] (4) Freeze-dry the wet gel in step (3) to obtain a superporous hydrogel sample, and sterilize it by Co-60 irradiati...

Embodiment 3

[0046] Example 3 Preparation of porous hydrogel

[0047] (1) Dissolve the water-soluble protein BSA in 10 mL of distilled water to obtain a BSA solution with a concentration of 100 mg / mL, and add the cross-linking agent β-diimine zinc complex and 1, 2, 7, 8 with a concentration of 1%. -Diepoxy octane each 2 mL, then add 10mg / mL NaH 2 PO 4 Solution 2mL, mix well, and adjust the pH of the solution to 4 with dilute hydrochloric acid;

[0048] (2) Dispense the mixed solution in step (1) into a mold, keep it at 50°C for 2 hours, then transfer it to a high-pressure steam sterilizer and keep it at 121°C for 20 minutes to obtain the initial gel;

[0049] (3) The first gel in step (2) is soaked and washed with distilled water for 5 days, and the washing water is changed every 6 hours to remove salt and residual crosslinking agent; transfer the washed first gel to the autoclave Continue to keep at 121°C for 2 h, then soak and wash again with distilled water for 2 days to remove the residual c...

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Abstract

The invention discloses a preparation method of a novel superporous hydrogel. First, dissolve the macromolecular protein in ultrapure water to prepare a protein solution, then add inorganic salts and cross-linking agents β-diimine zinc complex and 1,2,7,8-dioxoctane to adjust the concentration of the solution. When the pH is 2-5.5, stir evenly, heat in a water bath at 40-80°C for 0.5-5h, and dry after repeated high-temperature steam treatment and water washing to obtain a super-porous hydrogel. The porosity of the hydrogel is 75-90%, the maximum compressive strain and compressive stress can reach 70% and 5.5MPa respectively, and the swelling equilibrium can be reached in 5-10s. The porous hydrogel of the invention has good biocompatibility, and can be used as a drug slow-release carrier, a tissue filling material, a separation and purification medium, and the like.

Description

Technical field [0001] The invention relates to a method for preparing a novel superporous hydrogel, in particular to the preparation of a superporous hydrogel based on water-soluble macromolecular proteins. Background technique [0002] Hydrogel is a high-molecular polymer with a three-dimensional network structure that is rich in water and can be used in tissue engineering filling and drug slow-release fields. The mechanical properties and swelling rate of conventionally prepared hydrogels are generally poor, which limits their application range. Therefore, the design and preparation of (super) porous hydrogels with good mechanical strength and high swelling rate has become a major issue in recent years. Research hotspots. [0003] Porous hydrogels have obvious pore structure and can quickly achieve swelling balance by absorbing water through capillary action. The introduction of porous structure often directly leads to a significant decrease in the mechanical properties of the...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08J3/075C08J3/24C08J9/04C08L89/00
CPCC08J3/075C08J3/24C08J9/04C08J2389/00C08L89/00
Inventor 惠俊峰范代娣米钰马沛郑晓燕姜西娟
Owner NORTHWEST UNIV
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