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A high copper-resistant and high iron-resistant Leptospira siderophilus byl and its application

A technology of Leptospira ferrous and copper resistance, applied in the field of microorganisms, can solve the problem of not showing high-concentration iron ion and copper ion tolerance, not showing high-concentration copper ion and iron ion tolerance, affecting metal leaching And other issues

Active Publication Date: 2020-08-14
LANZHOU UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, as the leaching progresses, the concentration of metal ions in the leaching solution gradually increases, and high concentrations of metal ions have a strong inhibitory effect on the growth of microorganisms, thus affecting the leaching of metals.
[0003] In Chinese patent CN201410373597, a strain of Leptospira ferrophilic which is resistant to nickel ions is disclosed. The strain can grow in a solution of high concentration of nickel ions, but the strain does not show tolerance to high concentrations of iron ions and copper ions sex
In Chinese patent CN200510127741, a strain of fluoride-resistant Leptospira ferrophilum is disclosed. This strain can grow normally in an environment with a fluoride ion concentration of 1.2-1.4g / L, but the bacteria does not show resistance to high-concentration copper. Tolerance to ions and iron ions

Method used

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  • A high copper-resistant and high iron-resistant Leptospira siderophilus byl and its application
  • A high copper-resistant and high iron-resistant Leptospira siderophilus byl and its application
  • A high copper-resistant and high iron-resistant Leptospira siderophilus byl and its application

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Experimental program
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Effect test

Embodiment 1

[0019] Embodiment 1 Isolation and identification of bacterial strains of the present invention

[0020] (1) Take 1ml of water sample from the pit water of Baiyin deep copper mine and inoculate it to contain 20g / L FeSO 4 ·7H 2 O, in 9k medium with pH 2.5-2.8, 30°C enrichment culture for 5 days. Inoculate the bacterial solution obtained from the above-mentioned enrichment culture to a concentration of 60g / L FeSO 4 ·7H 2 O, in 9k medium with pH 2.5-2.8, cultured at 40°C, pH 1.8, passaged once every 3 days, to obtain a bacterial liquid containing Leptospira. Then transfer the above bacterial solution to pH1.6 containing 60g / L FeSO 4 ·7H 2 O, 28g / L ferric sulfate, cultivated in the 9k subculture medium of pH2.5-2.8 for 3 days, and serially diluted and passaged for 3 times (passage gradient is 10 -1 , 10 -2 , 10 -3 , 10 -4 , 10 -5 , 10 -6 , 10 -7 , 10 -8 ), and then transfer the last grown bacteria to the 9k subculture medium with pH 0.8, and then transfer to the 9k sub...

Embodiment 2

[0026] Embodiment 2 Growth characteristics of bacterial strains of the present invention

[0027](1) Take substratum 2 as bacterial growth substratum, add 50ml substratum in 250ml Erlenmeyer flask, the inoculum size of Leptospira ferrophilum BYL is 10%, adjusting pH is 1.0, 1.2, 1.4, 1.6, 1.8, 2.0; 180rpm, cultured at 40°C for 36h, and the iron oxidation rate was determined by potassium dichromate titration. Iron Oxidation Rate IOR (Iron Oxidation Rate) as figure 2 As shown, the optimum pH is 1.4.

[0028] (2) Take culture medium 2 as bacterial growth medium, add 50ml culture medium in 250ml Erlenmeyer flask, the inoculum size of Leptospira ferrophilum BYL is 10%, adjust pH1.4, at temperature be 30 ℃, 35 ℃ , 40°C, 45°C, and measure the iron oxidation rate. The result is as image 3 As shown, the optimum growth temperature range of the bacteria is 35-40°C, and it has oxidation ability at 45°C.

Embodiment 3

[0029] Example 3 Oxidation and tolerance of bacterial strains of the present invention to high-concentration iron ions

[0030] Change the concentration of ferrous sulfate in medium 2 to the following gradient (g / L): 10, 20, 40, 60, 80, 100, 120, 140, 160, 180, 200, 220, 240, 260, 280, 300, 320, 340, 360, 380, 400, the pH value was adjusted to 1.3, and 10 mL of medium with different concentration gradients was used to inoculate Leptospira ferrophilum BYL according to the inoculum size of 10% (v / v), and cultured with shaking at 40°C. After 36 h of oxidation, the concentration of ferrous ions in the culture was measured. get as Figure 4 Shown result, when growing in the culture medium that ferrous sulfate concentration range is 10-220g / L, along with the raising of iron ion concentration, iron oxidation efficiency also increases thereupon; When growing in the culture medium of / L, with the increase of iron ion concentration, the iron oxidation efficiency is obviously reduced. ...

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Abstract

The invention discloses a strain of Leptospirillum ferriphilium BYL (Leptospirillum ferriphilium BYL) with high copper resistance and high iron resistance, which has been preserved in the China Center for Type Culture Collection on April 19, 2015, and the preservation number is CCTCC NO : M 2015242, named Leptospirillum ferriphilium BYL (Leptospirillum ferriphilium BYL). The invention also discloses the oxidation properties of Leptospirillum ferriphilium BYL (Leptospirillum ferriphilium BYL) to high-concentration iron ions and the tolerance to high-concentration copper ions. The bacteria can be widely used in sulfide ore leaching and hydrogen sulfide removal .

Description

technical field [0001] The invention belongs to the field of microbes, and in particular relates to a high copper-resistant and high iron-resistant Leptospirillum ferriphilium BYL (Leptospirillum ferriphilium BYL) and its application in sulfide ore leaching and hydrogen sulfide removal. The fungus has been preserved in the China Center for Type Culture Collection on April 19, 2015, address: China. Wuhan. Wuhan University, Zip Code: 430072, Tel: (027)68754052, Fax: (027)68754833, E-mail : cctcc@whu.edu.cn, its deposit number is CCTCC NO: M 2015242. Background technique [0002] Bioleaching is a technology to recover metals from low-grade ore and refractory ore. This technology has little environmental pollution and low energy consumption. In this process, the most important thing is the efficient microbial leaching strain. Thiobacillus ferrooxidans and Leptospira ferrooxidans are currently considered to be the most potential microbial strains, which can oxidize ferrous ions...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C22B3/18B01D53/84B01D53/52C12R1/01
CPCB01D53/52B01D53/84B01D2257/304C22B3/18C12N1/205C12R2001/01Y02P10/20Y02P20/59Y02A50/20
Inventor 李红玉刘荣辉李洋赵晶戴治娟蒋云龙
Owner LANZHOU UNIVERSITY