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PDE-5 inhibitor colloidal gold immunochromatographic test strip and preparation and application methods thereof

An immunochromatography test strip, PDE-5 technology, applied in the field of PDE-5 inhibitor colloidal gold immunochromatography test strips, can solve the problems of exceeding the safe use dose, health threats, practical application inconvenience, etc., and reach the detection range Wide, high sensitivity, and the effect of expanding the detection range

Inactive Publication Date: 2017-10-27
广东志道医药科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] ①The interaction between PDE-5 inhibitors and nitrate drugs can lead to severe hypotension; ②The analogues of PDE-5 inhibitors have not been clinically studied, and there are unknown risk factors; ③The dosage of illegally added PDE-5 drugs often exceeds the safe dosage. serious threat to health
[0007] At present, there are some colloidal gold immunochromatographic test strips (such as sildenafil) for detecting PDE-5 inhibitors on the market, but most of them can only detect one component at the same time, which brings inconvenience to practical application

Method used

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  • PDE-5 inhibitor colloidal gold immunochromatographic test strip and preparation and application methods thereof
  • PDE-5 inhibitor colloidal gold immunochromatographic test strip and preparation and application methods thereof
  • PDE-5 inhibitor colloidal gold immunochromatographic test strip and preparation and application methods thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Example 1: Preparation of general hapten-carrier protein conjugates:

[0043] Take the preparation of NFA-BSA conjugates as an example: the general-purpose hapten of nafil used in the present invention contains a carboxyl group, and the carboxyl group is combined with the amino group in the carrier protein by the carbodiimide method, and the nafil The general-purpose hapten is coupled to the carrier protein, and the specific operation is as follows: Weigh 0.1069g NFA, 0.0345g NHS and 0.0575g EDC, dissolve it in 3mL DMSO, and stir it overnight with magnetic force to obtain the EDC solution. Weigh 0.12g of BSA, dissolve it in 5mL of sodium bicarbonate buffer solution with a concentration of 0.1mol / L and pH7.0 to obtain a BSA solution, slowly add the EDC solution dropwise to the BSA solution under stirring, and react overnight, The obtained solution was dialyzed with distilled water at 4°C for 5 days, the dialysate was changed every 8 hours every day, and finally the solut...

Embodiment 2

[0044] Embodiment 2: application embodiment:

[0045] Take about 1 / 2 of commercially available aphrodisiac health care products (capsules), add 5% ethanol aqueous solution to the content, shake and extract, place it in layers, absorb the supernatant, and add it dropwise to the colloidal gold immune layer of Nafil and Lafia Put it on the sample pad of the analysis test strip, react for 5-10 minutes, observe the color development of the detection line and the control line, if the detection line and the control line are both red, the result is negative, indicating that the sample does not contain nafil and diafil If the test line does not show red, but the control line shows red, the result is positive, indicating that the sample contains nafil or diafil components; if the control line does not show red, it means that the test strip is invalid, and a new one needs to be used Test strips for testing.

Embodiment 3

[0046] Embodiment 3: sensitivity test:

[0047] 9 kinds of nafiloid components and 3 kinds of nafiloid ingredients are detected with test strip of the present invention, and every kind of composition is all formulated into different concentrations, as shown in table 1:

[0048] Table 1 test strip sensitivity and specificity test results

[0049]

[0050]

[0051] The results showed that sildenafil, reddenafil, vardenafil, pseudovardenafil, homosildenafil, hydroxyhomosildenafil, reddenafil, namoxidenafil The test results of 9 kinds of nafil-like ingredients such as phenafil and thioedenafil were all positive, and the minimum detection limit was between 0.2 mg / kg and 2 mg / kg. Among the 3 kinds of nafil-like ingredients, tadalafil, aminotafil The detection results of Dalafil and Diethylaminopretadalafil are all positive, and the minimum detection limit is between 0.1mg / kg~5mg / kg, illustrating that the test strip of the present invention can be used for most Nafil class com...

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Abstract

The invention discloses a PDE-5 inhibitor colloidal gold immunochromatographic test strip, which comprises a PVC base plate, a sample pad, a gold label pad, a detection line, a control line, a cellulose nitrate membrane and an absorbent pad, wherein the sample pad is arranged at the edge of one end of the PVC base plate; the gold label pad is arranged at the inner side of the sample pad and partially overlaps with the sample pad; the gold label pad is coated with a colloidal gold-labeled non generic antibody and Lafay generic antibody mixture; the absorbent pad is arranged at the other end of the PVC base plate; the cellulose nitrate membrane is arranged between the gold label pad and the absorbent pad and partially overlaps with the gold label pad and the absorbent pad separately; the detection line is arranged at one end, close to the gold label pad, on the cellulose nitrate membrane; and the control line is sprayed at one end close to the absorbent pad. The test strip is capable of detecting most of non ingredient and Lafay ingredient, is high in sensitivity and good in stability, and can be applied to fast detection and initial screening of the non ingredient and the Lafay ingredient in products, such as yang-tonifying health products and Chinese patent medicines.

Description

technical field [0001] The invention relates to the field of detection of nafils and diafils, in particular to a PDE-5 inhibitor colloidal gold immunochromatographic test strip and a preparation and use method thereof. Background technique [0002] Phosphodiesterase type 5 (PDE-5) belongs to the cyclic nucleotide hydrolase family, mainly exists in the penis cavernous body, smooth muscle and platelets, can specifically bind to cyclic guanylic acid, and is the key to the metabolism of cyclic guanylic acid in the penis. Inactive major isozyme. PDE-5 inhibitors can selectively inhibit the activity of PDE-5 present in the cavernous body of the penis, maintain the local concentration of cyclic guanylic acid at a high level, promote the relaxation of smooth muscle in the cavernous body, and increase blood flow to achieve the effect of treating impotence . Currently, the approved PDE-5 inhibitors mainly include sildenafil, vardenafil and tadalafil. [0003] In recent years, scien...

Claims

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Application Information

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IPC IPC(8): G01N33/573
CPCG01N33/573
Inventor 冯锐龙飞罗莉妮杨鹏博
Owner 广东志道医药科技有限公司