Growth factor-loaded microsphere composite 3D printed scaffold, and preparation method thereof

Abstract

The invention provides a preparation method for a growth factor-loaded microsphere composite 3D printed scaffold. The preparation method comprises the following steps: providing a composite microsphere loaded with growth factors and a synthesis biomaterial solution, adding the composite microsphere loaded with growth factors into the synthesis biomaterial solution and carrying out uniform mixing under stirring so as to form a synthesis biomaterial / microsphere solution; designing the printing parameters of the 3D composite scaffold by using the Cark software of a LDM system, successively connecting a material tank, a feeding pipe and a nozzle, and pouring the synthesis biomaterial / microsphere solution into the material tank; starting a temperature control device and a numerical control device when the temperature of a molding chamber drops to -25 to -35 DEG C, starting printing and shaping according to shaping parameters set by the Cark software and carrying out layer-upon-layer superposition so as to form a three-dimensional frozen scaffold; and taking out the three-dimensional frozen scaffold and carrying out freeze drying so as to obtain the growth factor-loaded microsphere composite 3D printed scaffold.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to a 3D printing scaffold compositely loaded with growth factor microspheres and a preparation method thereof. Background technique [0002] Cartilage defects are caused by sports injuries, inflammation, tumors and natural degeneration. The self-repair ability of cartilage defect is limited, especially articular cartilage, which cannot heal itself after injury. It is estimated that in the United States alone, by 2020, there will be 40 million cases of related cartilage defects. China is a country with a large population, and the incidence rate of cartilage injury among the people undergoing knee arthroscopy is between 61-68%, and it shows a trend of increasing year by year. At present, the commonly used clinical treatment methods mainly include microfracture, autologous chondrocyte transplantation, and artificial joint replacement. These repair methods have their own shortco...

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Problems solved by technology

[0005] The purpose of the present invention is to provide a 3D printing scaffold compositely loaded with growth factor microspheres and its preparation method, aiming to solve the problem that the existing LDM molding scaffold is difficult to solve the problem of long-term stable release of growth factors

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