Phycocyanin hypoglycemic polypeptide

A phycocyanin and hypoglycemic technology, which is applied in the fields of peptides, depsipeptides, cardiovascular system diseases, etc., can solve the problems of loss of active ingredients of spirulina dry powder, inability to eliminate the fishy smell of spirulina, and low yield of active polypeptides. The effect of avoiding protein denaturation, shortening extraction time, and improving peptide yield

Inactive Publication Date: 2017-11-03
浦江县欧立生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The current preparation of spirulina polypeptide is usually to use spirulina dry powder to add water to prepare a suspension, and then use bio-enzyme engineering technology to hydrolyze spirulina protein to prepare spirulina polypeptide, but the active ingredients of spirulina dry powder have been lost in a large amount, so that the final product has insufficient activity. The extraction rate is low, and the fishy smell of spirulina cannot be eliminated
The invention uses living spirulina as ra...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] A phycocyanin hypoglycemic polypeptide, the preparation steps of the hypoglycemic polypeptide are:

[0016] 1) Cell crushing: prepare algae liquid, the solid-to-liquid ratio of spirulina dry powder to water is 1:50, add 0.07% functional protein peptide of the weight of spirulina dry powder to the algae liquid, choose sodium phosphate buffer as the extraction solvent, The pH value of the sodium phosphate buffer solution is 7.0, at -20°C and 37°C, freeze-thaw repeatedly 5 times, 4h each time, centrifuge at 5000rpm for 30min, collect the supernatant to obtain the crude extract of phycocyanin, place in Store in the refrigerator at 4°C for later use; the amino acid sequence of the functional protein peptide is MKGLSFVLLVLLLLMMPDGEGDPEMQYWTGRGLCRRFCYEYFVGHHCPRRYRCCAIRA;

[0017] 2) Salting out: add (NH 4 ) 2 SO 4 The solution was brought to 80% saturation, left standing at 4°C for 12 hours, and centrifuged at 6000rpm for 15 minutes to obtain the salt-out precipitate;

[0...

Embodiment 2

[0023] A phycocyanin hypoglycemic polypeptide, the preparation steps of the hypoglycemic polypeptide are:

[0024] 1) Cell crushing: prepare algae liquid, the solid-liquid ratio of spirulina dry powder to water is 1:50, add 0.08% functional protein peptide of the weight of spirulina dry powder to the algae liquid, choose sodium phosphate buffer as the extraction solvent, The pH value of the sodium phosphate buffer solution is 7.0, at -20°C and 37°C, freeze-thaw repeatedly 5 times, 4h each time, centrifuge at 5000rpm for 30min, collect the supernatant to obtain the crude extract of phycocyanin, place in Store in the refrigerator at 4°C for later use; the amino acid sequence of the functional protein peptide is MKGLSFVLLVLLLLMMPDGEGDPEMQYWTGRGLCRRFCYEYFVGHHCPRRYRCCAIRA. The active peptide is hydrophilic and lipophilic. The hydrophilicity makes it soluble in distilled water, and the lipophilicity makes it combine with the cell membrane of Spirulina to form small holes under the c...

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PUM

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Abstract

The invention discloses a phycocyanin hypoglycemic polypeptide. The phycocyanin hypoglycemic polypeptide is obtained after the following steps: 1) cell disruption: adding a functional protein peptide into an algae solution, selecting a sodium phosphate buffer solution as an extraction solvent, and performing repeated freezing and thawing and centrifugation to obtain a supernatant namely a protein crude extract; 2) salting-out: adding a (NH4)2SO4 solution into the protein crude extract, performing standing at 3-5 DEG C for 11-13h, and performing centrifugation to obtain a salting-out sediment; 3) aqueous two-phase extraction: mixing glycol and an inorganic salt until the pH value is 5.8-6.5, adding the salting-out sediment, performing magnetic stirring, then transferring the mixture into a separating funnel for layering to obtain an upper phase namely a protein solution; 4) ultrafiltration: putting the protein solution into an ultrafiltration centrifuge tube, and performing centrifugation and freeze-drying to obtain the phycocyanin hypoglycemic polypeptide. The phycocyanin hypoglycemic polypeptide disclosed by the invention has functions of lowering blood pressure and blood fat and activating blood vessels, and the polypeptide yield is significantly improved via cell disruption during preparation, so that the polypeptide yield is high, and the phycocyanin hypoglycemic polypeptide can be applied to foods or beverages.

Description

technical field [0001] The invention belongs to the technical field of natural product protein extraction, in particular to a phycocyanin hypoglycemic polypeptide. Background technique [0002] According to the International Diabetes Federation (IDE) report in Diabetes Atlas in 2006, diabetes has spread globally. At present, diabetes affects 246 million people in the world, accounting for 5.9% of the global population, and nearly 80% of the total number of patients are in developing countries, including 40.9 million in India and 39.8 million in China. The report predicts that if no measures are taken in the next 20 years, the number of patients suffering from diabetes will increase to 380 million people. The prevalence of diabetes worldwide and the rapid development of new drugs for treating diabetes will increase the market for diabetes drugs by 16% every year, reaching 20 billion US dollars in 2006. Diabetes patients are mainly divided into two types: insulin-dependent d...

Claims

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Application Information

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IPC IPC(8): C07K14/795C07K1/34C07K1/30C07K1/14C12N1/06A61P3/10A61P3/06A61P9/14A23L33/18
Inventor 李冰
Owner 浦江县欧立生物技术有限公司
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