Method for efficiently extracting phenol-based extract from pyroligneous acid

Abstract

The present invention relates to a method for efficiently extracting a phenol-based extract from a pyroligneous acid. The method comprises: extracting a pyroligneous acid three times with ethyl acetate, and combining the obtained extraction liquids to obtain a first-stage pyroligneous acid; extracting the first-stage a pyroligneous acid three times by using a 0.6 mol / L NaHCO3 solution, and combining the obtained extraction liquids to obtain a second-stage pyroligneous acid; extracting the second-stage pyroligneous acid twice by using a 1 mol / L sodium hydroxide solution, combining the obtained extraction liquids to obtain a crude phenol extract, adding concentrated phosphoric acid to the crude phenol extract, and adjusting the PH value to obtain a third-stage pyroligneous acid; adding a Na2HPO4 solution with a mass concentration of 5%, 10%, 15% or 20%, uniformly shaking, and extracting with a mixed liquid to obtain a four-stage pyroligneous acid; and carrying out rotary evaporation on the four-stage pyroligneous acid, and drying to obtain the phenol-based extract. The invention provides the method for efficiently extracting the phenol-based extract from the pyroligneous acid, wherein the proportion of the phenol-based extract is optimized, and the results prove that the method is practicable.

Description

technical field [0001] The invention relates to a method for efficiently extracting phenolic extracts from wood vinegar. Background technique [0002] Wood vinegar is a yellow-brown liquid rich in substances such as acids, phenols, and ketones. In recent years, wood vinegar has been widely used in the fields of biomedicine and cosmetics. Scientists have found that wood vinegar has strong bactericidal and antioxidant properties. At the same time, Ho et al. found that wood vinegar has strong anti-inflammatory properties both in vivo and in vitro in 2013, which confirmed that wood vinegar can be used for the treatment of NLRP3 and metabolic syndrome. The above findings have laid a solid theoretical foundation for the wide application of wood vinegar in the field of biomedicine. Loo et al. confirmed in 2007 that phenolic extracts are the main antioxidant substances in wood vinegar. At the same time, Shinsuke confirmed in 2012 that phenolic extracts are the main bactericidal ...

AI Technical Summary

Problems solved by technology

However, this method will introduce a large amount of impurities such as acetic acid and furfural into the phenolic extract, and cannot be directly applied in the field of medicine

Although Shinsuke obtained relatively pure phenolic extracts by combining ethyl acetate, sodium bicarbonate, and column purification, this method is expensive and inevitably brings in a large amount of furfural

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