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Pantoea ananatis promoting dissolution of ground phosphate rock and applications thereof

A technology of Pantoea pineapple and phosphate rock powder, applied in the field of microorganisms, can solve the problems of low grade of phosphate rock powder, weakened pollution control effect, and poor solubility

Active Publication Date: 2017-11-10
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, when phosphate rock powder is used to passivate heavy metals in farmland soil, due to the low grade and insoluble problems of phosphate rock powder, phosphorus cannot be released effectively, and the effect of pollution control is greatly weakened.

Method used

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  • Pantoea ananatis promoting dissolution of ground phosphate rock and applications thereof
  • Pantoea ananatis promoting dissolution of ground phosphate rock and applications thereof
  • Pantoea ananatis promoting dissolution of ground phosphate rock and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Embodiment 1: Isolation and purification of phosphate-dissolving bacteria

[0024] First carry out surface disinfection (95% alcohol (1min) → 30% hydrogen peroxide + 3% sodium hypochlorite (20min) → 95% alcohol (1min) → rinse with sterile water three times) to the root of Sedum sedum, and put the root tissue after disinfection. In a sterilized mortar, grind it into juice, add an appropriate amount of phosphate buffer solution, stir evenly, let it stand for 10 minutes, stir again, and dilute Sedum sedum and tissue grinding liquid with sterilized water. Pipette 200 μL from the stock solution and the diluted solution into LB solid medium for culture. After one week of plate culture, count and record the morphology of the colonies. At the same time, according to the characteristics of the colonies, different colonies were selected for new culture, purified once, and stored at -80°C.

Embodiment 2

[0025] Embodiment 2: the screening of phosphate-dissolving bacteria

[0026] Qualitative experiment: After activating the strain preserved in Example 1 on LB liquid medium, take 10 μL of the bacterial liquid and spot it in NBRIP (National Botanical Research Institute's phosphate growth medium) solid medium, and place it at 30°C with constant temperature and humidity Cultivate in the incubator for 7 days, if there is an obvious bright circle around the colony, it means that the strain is a phosphate lysing bacteria.

[0027] Quantitative experiment: After the bacterial strain preserved in Example 1 is activated on the LB liquid medium, inoculate 0.5mL of the bacterial liquid of the bacterial strain in the logarithmic growth phase into the 50mL NBRIP liquid medium, and repeat 3 times for each treatment, while adding sterile Ultrapure water was used instead of bacterial solution as blank control, (28°C, 180r·min -1 ) Shaker for 7 days, centrifuged (5 000r·min -1 ) for 10 min, t...

Embodiment 3

[0032] Example 3: Screening and identification of phosphate-dissolving bacteria

[0033]Species identification, individual morphological observation and physiological and biochemical characteristics were carried out on the screened phosphate-dissolving bacteria. The identification and morphological observation of the bacteria in this study were completed by the Guangdong Provincial Microbiological Analysis and Testing Center. The base sequence of the strain was obtained through the Internet in GenBank, etc. Perform a homologous sequence search (blast search) in the international nucleic acid sequence database to find the type strain with the highest homology between the strain and the database or strains preserved in international culture collection centers such as ATCC or DSM.

[0034] (1) Morphological characteristics of bacteria

[0035] Gram-negative bacteria. The bacteria are rod-shaped and transformed to anaerobic. The transmission electron microscope characteristic ma...

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Abstract

The invention belongs to the technical field of microbes, and particularly relates to pantoea ananatis promoting dissolution of ground phosphate rock and applications thereof. The pantoea ananatis HCR2 is deposited in the Guangdong Culture Collection Center on January 16, 2017. The accession number is GDMCC No:60140. The pantoea ananatis can effectively dissolve ground phosphate rock, and can promote a function of the ground phosphate rock for immobilizing lead in soil or solutions.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a kind of Pantoea ananatis which promotes the dissolution of phosphate rock powder and an application thereof. Background technique [0002] At present, the overall situation of my country's soil environment is not optimistic, some areas are seriously polluted, the quality of cultivated land soil environment is worrying, and the soil environment problems of industrial and mining abandoned land are prominent. Human activities such as industrial mining, agriculture and high background value of soil environment are the causes of soil pollution or exceeding the standard. the main reason. Among them, lead polluted soil is more serious. Lead is a highly toxic heavy metal. Once it enters the human body, it will be difficult to get rid of. It can directly damage human brain cells, especially the nervous system of the fetus, which can cause congenital mental retardation...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20B09C1/10B09C1/08C12R1/01
CPCB09C1/08B09C1/10B09C2101/00C12N1/205C12R2001/01
Inventor 龙新宪黄莉敏游敏萍
Owner SOUTH CHINA AGRI UNIV
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