Two-point integral fluorescence lifetime rapid detection system

Abstract

The invention discloses a two-point integral fluorescence life rapid detection system. The two-point integral fluorescence life rapid detection system comprises an oxygen sensitive fluorescent film, an excitation light source, a photoelectric detection circuit, a detector temperature control circuit, and a master controller. According to the two-point integral fluorescence life rapid detection system, an LED is taken as a light source; a silicon photoelectric multiplier is taken as a detector; a fluorescence attenuation curve is amplified automatically by combining a variable gain amplifier; fluorescence intensity at different times is measured rapidly by adopting a high-speed switch type charge integrator, so that rapid high-sensitivity measurement of multi-point fluorescence intensity of the fluorescence attenuation curve in a fluorescence quenching process is realized; the fluorescence life of the oxygen sensitive fluorescent film is directly inverted by selecting the intensity of two points of the fluorescence attenuation curve, and the dissolved oxygen concentration is obtained. The two-point integral fluorescence life rapid detection system has the characteristics that the measurement is rapid, a detecting circuit is simple and easy to realize and is not easily interfered by external factors, such as light source fluctuation, aging and natural lighting, the measurement is stable, the reliability is high and the like.

Description

technical field [0001] The invention mainly relates to the fields of resources, environment and ocean, in particular to a two-point integral fluorescence lifetime rapid detection system. Background technique [0002] Dissolved oxygen in water refers to the molecular oxygen dissolved in water, which is an indispensable condition for the survival of aquatic organisms and an important parameter to measure aquatic ecosystems. The level of dissolved oxygen can reflect water pollution, especially the degree of organic pollution, so it is also an important comprehensive evaluation index of water quality. Accurate and rapid acquisition of dissolved oxygen concentration information in water bodies is crucial to mastering the ecological environment of water bodies, ensuring drinking water safety, and developing aquaculture. [0003] At present, the commonly used dissolved oxygen detection methods mainly include iodometric method, electrode method, fluorescence quenching method and so...

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Problems solved by technology

[0004] At present, most of the dissolved oxygen detection is to measure the fluorescence lifetime by the frequency domain phase method, which is detected by converting the fluorescence lifetime in the time domain into the lag phase of the fluorescence relative to the excitation light in the frequency domain. For example, ZL201010580318.3 discloses a digital phase lock method Fluorescence lifetime detection technology, but the frequency-domain fluorescence lifetime method for water dissolved oxygen concentration detection technology has problems such as demanding excitation light source, complex phase detection circuit, and long phase difference stabilization period.

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