Traditional Chinese medicine fermentation preparation for improving growth performance and immunity of litopenaeus vannamei, and preparing method and application thereof

The technology of fermentation preparation and traditional Chinese medicine is applied in the field of traditional Chinese medicine fermentation preparation for improving the growth performance and immunity of Litopenaeus vannamei, and can solve the problems affecting the healthy and sustainable development of the breeding industry of Litopenaeus vannamei, the deterioration of the breeding environment, the degradation of seed resources and the like. , to achieve the effect of improving growth performance and immunity, fast growth and strong resistance to stress

Inactive Publication Date: 2017-12-01
哈尔滨中科生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the breeding area and scale of Litopenaeus vannamei in my country are expanding year by year, the degradation of seed resources, the low survival rate of prawns caused by infectious diseases, the high cost of breed

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Preparation of bacterial solution

[0037] The above-mentioned Pediococcus lactis zk160135 strain was inoculated in the activated medium for cultivation at a temperature of 37°C for 24 hours; the activated strain was inoculated in a fermenter at a temperature of 35°C to 40°C for 18h to 24h. Prepare the fermentation broth.

[0038] The activation medium is: MRS medium, the composition is (g / L): peptone 10.0, meat extract 8.0, yeast extract powder 4.0, glucose 20.0, dipotassium hydrogen phosphate 2.0, diammonium hydrogen citrate 2.0, sodium acetate 5.0 , magnesium sulfate 0.2, manganese sulfate 0.04, Tween 80 1.0, adjust the pH to 6.2-6.4. Sterilize at 115°C for 20min.

[0039] The medium used for fermentation is: MRS medium, the composition of which is the same as above.

[0040] After the fermentation is completed, the bacterial content in the bacterial liquid is ≥100 million / mL.

Embodiment 2

[0042] A fermented traditional Chinese medicine preparation for improving the growth performance and immunity of Litopenaeus vannamei, the traditional Chinese medicine components of which are composed of: 1.5 parts of Pulsatillae extract, 0.8 part of Coptis chinensis extract, 1.0 part of Cortex Phellodendri extract, and 1.2 parts of Qinpi extract by weight. 0.7 parts of hawthorn extract, 0.2 parts of woody extract, 0.5 parts of nutmeg extract, and 1.5 parts of licorice extract.

[0043] The traditional Chinese medicine extract was purchased from Sichuan Hengrui Tongda Biotechnology Co., Ltd.

[0044] The specific preparation method is as follows:

[0045](1) Take each Chinese medicine extract according to the amount;

[0046] (2) Add MRS liquid medium whose weight is 10 times that of the traditional Chinese medicine extract, heat to 70°C and stir for 15 minutes, adjust the pH to 6.8, steam sterilize at 100°C for 30 minutes, and cool to 30°C-35°C;

[0047] (3) Add the bacteri...

Embodiment 3

[0049] A fermented traditional Chinese medicine preparation for improving the growth performance and immunity of Litopenaeus vannamei, the Chinese medicine components of which are composed of: 1.2 parts of Pulsatillae extract, 0.5 part of Coptis chinensis extract, 0.8 part of Cortex Phellodendri extract, and 0.8 part of Qinpi extract by weight. 0.5 parts of hawthorn extract, 0.1 part of woody extract, 0.4 part of nutmeg extract, and 1.0 part of licorice extract.

[0050] The preparation method is the same as in Example 2.

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PUM

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Abstract

The invention discloses a traditional Chinese medicine fermentation preparation for improving growth performance and immunity of litopenaeus vannamei, and a preparing method and application thereof, and belongs to the technical field of traditional Chinese medicine fermentation preparations. The traditional Chinese medicine fermentation preparation is prepared by fermenting of a mixture of traditional Chinese medicine components and pediococcus acidilactici bacteria liquid. The traditional Chinese medicine components comprise, by weight, 1.2-2.0 parts of pulsatilla extract, 0.5-1.0 part of coptis chinensis extract, 0.8-1.2 parts of cortex phellodendri extract, 0.8-1.5 parts of cortex fraxini extract, 0.4-0.7 part of nutmeg extract, 0.5-1.0 part of hawthorn extract, 0.1-0.3 part of costusroot extract and 1.0-2.0 parts of liquorice extract. According to the traditional Chinese medicine fermentation preparation for improving growth performance and immunity of litopenaeus vannamei, the preparing method and the application thereof, selected traditional Chinese medicine extracts are reasonable in structure and proper in proportion; selected pediococcus acidilactici is high in activity and stress resistance, and the efficacy of an original medicine is improved remarkably; and the growth performance and immunity of the litopenaeus vannamei can be improved significantly.

Description

technical field [0001] The invention belongs to the technical field of traditional Chinese medicine fermented preparations, and in particular relates to a traditional Chinese medicine fermented preparation for improving the growth performance and immunity of Litopenaeus vannamei, its preparation method and application. Background technique [0002] In recent years, my country's shrimp farming industry has developed rapidly. Although the breeding area and scale of Litopenaeus vannamei in my country are expanding year by year, the degradation of seed resources, the low survival rate of prawns caused by infectious diseases, the high cost of breeding, and the deterioration of the breeding environment have brought huge losses to shrimp farmers. Economic loss has seriously affected the healthy and sustainable development of Litopenaeus vannamei aquaculture. Although the application of antibiotics has achieved certain results, the resulting drug resistance and harm to the environme...

Claims

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Application Information

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IPC IPC(8): A61K36/756A61P37/04A23K10/18A23K10/12A23K10/30A23K50/80A61K35/741
CPCA61K35/741A61K36/185A61K36/285A61K36/484A61K36/63A61K36/71A61K36/718A61K36/734A61K36/756A61K2236/19A61K2300/00Y02A40/818
Inventor 隋利涛曹亚彬梁晓彤
Owner 哈尔滨中科生物工程有限公司
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