Traditional Chinese medicine fermentation preparation for improving growth performance and immunity of litopenaeus vannamei, and preparing method and application thereof
The technology of fermentation preparation and traditional Chinese medicine is applied in the field of traditional Chinese medicine fermentation preparation for improving the growth performance and immunity of Litopenaeus vannamei, and can solve the problems affecting the healthy and sustainable development of the breeding industry of Litopenaeus vannamei, the deterioration of the breeding environment, the degradation of seed resources and the like. , to achieve the effect of improving growth performance and immunity, fast growth and strong resistance to stress
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Embodiment 1
[0036] Preparation of bacterial solution
[0037] The above-mentioned Pediococcus lactis zk160135 strain was inoculated in the activated medium for cultivation at a temperature of 37°C for 24 hours; the activated strain was inoculated in a fermenter at a temperature of 35°C to 40°C for 18h to 24h. Prepare the fermentation broth.
[0038] The activation medium is: MRS medium, the composition is (g / L): peptone 10.0, meat extract 8.0, yeast extract powder 4.0, glucose 20.0, dipotassium hydrogen phosphate 2.0, diammonium hydrogen citrate 2.0, sodium acetate 5.0 , magnesium sulfate 0.2, manganese sulfate 0.04, Tween 80 1.0, adjust the pH to 6.2-6.4. Sterilize at 115°C for 20min.
[0039] The medium used for fermentation is: MRS medium, the composition of which is the same as above.
[0040] After the fermentation is completed, the bacterial content in the bacterial liquid is ≥100 million / mL.
Embodiment 2
[0042] A fermented traditional Chinese medicine preparation for improving the growth performance and immunity of Litopenaeus vannamei, the traditional Chinese medicine components of which are composed of: 1.5 parts of Pulsatillae extract, 0.8 part of Coptis chinensis extract, 1.0 part of Cortex Phellodendri extract, and 1.2 parts of Qinpi extract by weight. 0.7 parts of hawthorn extract, 0.2 parts of woody extract, 0.5 parts of nutmeg extract, and 1.5 parts of licorice extract.
[0043] The traditional Chinese medicine extract was purchased from Sichuan Hengrui Tongda Biotechnology Co., Ltd.
[0044] The specific preparation method is as follows:
[0045](1) Take each Chinese medicine extract according to the amount;
[0046] (2) Add MRS liquid medium whose weight is 10 times that of the traditional Chinese medicine extract, heat to 70°C and stir for 15 minutes, adjust the pH to 6.8, steam sterilize at 100°C for 30 minutes, and cool to 30°C-35°C;
[0047] (3) Add the bacteri...
Embodiment 3
[0049] A fermented traditional Chinese medicine preparation for improving the growth performance and immunity of Litopenaeus vannamei, the Chinese medicine components of which are composed of: 1.2 parts of Pulsatillae extract, 0.5 part of Coptis chinensis extract, 0.8 part of Cortex Phellodendri extract, and 0.8 part of Qinpi extract by weight. 0.5 parts of hawthorn extract, 0.1 part of woody extract, 0.4 part of nutmeg extract, and 1.0 part of licorice extract.
[0050] The preparation method is the same as in Example 2.
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