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Method for inducing umbilical cord mesenchymal stem cell to differentiate to form insulin

A stem cell and insulin technology, applied in biochemical equipment and methods, pancreatic cells, animal cells, etc., can solve problems such as unsatisfactory treatment effects, and achieve the effect of less chance of infection and strong amplification ability

Inactive Publication Date: 2017-12-12
HENAN YINFENG BIOENG CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The current conventional treatment for diabetes is only symptomatic treatment. The best state is to make blood sugar control stable, the dosage is gradually increasing, and the islet function is gradually failing. Most of the current treatment effects are not ideal. With the development of stem cells and clinical transformation research In-depth, the use of stem cell-induced differentiation of islet cells to treat diabetes has become a new hope

Method used

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Embodiment 1

[0017] A method for inducing differentiation of umbilical cord mesenchymal stem cells to form insulin, comprising the following steps:

[0018] (1) In vitro isolation and culture of umbilical cord mesenchymal stem cells: Take umbilical cord blood, mix with an equal amount of PBS buffer, slowly drop into an equal amount of lymphocyte separation solution, and centrifuge at 650g for 15 minutes; extract buffy coat cells, and Wash and centrifuge with an equal amount of PBS buffer, resuspend the basal culture medium, inoculate into a 100mm culture dish, and store at 37°C, saturated humidity, 5% CO 2 cultured in an incubator; the first full amount of medium was changed on the 5th day, and then changed twice a week. After 2 weeks, when 60% to 80% confluence was reached, 0.25% trypsin was routinely digested and passaged; the composition of the basic culture medium was: DMEM / F12, 10% fetal bovine serum, 100 U / mL penicillin and streptomycin, 2 mmol / L L-glutamine.

[0019] (2) Cytokines...

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Abstract

The invention discloses a method for inducing an umbilical cord mesenchymal stem cell to differentiate to form insulin. The method comprises the following steps: separating and cultivating the umbilical cord mesenchymal stem cell in vitro; enabling a cell factor to induce the umbilical cord mesenchymal stem cell to differentiate into an insulin cell: inoculating a third generation umbilical cord mesenchymal stem cell in a culture flask T25cm, adding an inducing liquid when the cell is fused by 80%-90%, and inducing the cell to obtain the insulin cell. The differentiating capability of the umbilical cord mesenchymal stem cell obtained by separating and cultivating umbilical cord blood is relatively strong, and the umbilical cord mesenchymal stem cell has a multi-directional differentiation potential, supports hematopoiesis and has an immunosuppression effect. Compared with donating bone marrow or drawing and mobilizing peripheral blood, a donator has no pain, the risk of infection is reduced, and the amplification capability is strong.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for inducing differentiation of umbilical cord mesenchymal stem cells to form insulin. Background technique [0002] Umbilical cord mesenchymal stem cells (Mesenchymal Stem Cells, MSCs) refer to a kind of multifunctional stem cells existing in the umbilical cord tissue of newborns, which can differentiate into many kinds of tissue cells and have broad clinical application prospects. [0003] Umbilical cord mesenchymal stem cells have high differentiation potential and can differentiate in multiple directions. It has broad clinical application prospects in tissue engineering of bone, cartilage, muscle, tendon, ligament, nerve, liver, endothelium and cardiac muscle. It has been reported that mesenchymal stem cells were isolated from human umbilical cord, and the cell content and proliferation ability were superior to those of bone marrow mesenchymal stem cells, th...

Claims

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Application Information

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IPC IPC(8): C12N5/071C12N5/0775
CPCC12N5/0676C12N2500/25C12N2500/38C12N2501/11C12N2501/115C12N2501/13C12N2501/16C12N2506/1392
Inventor 朱学义聂江华王小龙陈乐陈艳普徐峰波
Owner HENAN YINFENG BIOENG CO LTD
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