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Apolipoprotein A1 detection kit and detection method

A detection kit and apolipoprotein technology are applied in the field of medical immunology in vitro diagnosis, which can solve the problems of inaccurate detection results of the reagents, inability to meet the requirements for use, and the coefficient of variation becomes large, and achieve good stability of the reagents, wide versatility, The effect of accurate detection results

Active Publication Date: 2017-12-12
SICHUAN MACCURA BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] Antibodies are prone to flocculent or flaky precipitation during storage, resulting in poor antibody performance, poor repeatability, and large coefficient of variation (CV), directly resulting in inaccurate reagent test results, and additional filtration steps, complicated operation, And filtration may remove antibodies, affect the detection effect, cause certain impact on patients, and cannot meet the requirements for use

Method used

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  • Apolipoprotein A1 detection kit and detection method
  • Apolipoprotein A1 detection kit and detection method
  • Apolipoprotein A1 detection kit and detection method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Example 1 Apolipoprotein A1 detection kit

[0044] Reagent R1:

[0045]

[0046]

[0047] Reagent R2:

[0048] TRIS buffer

20mmol / L

Sodium chloride

1g / L

Sodium azide

0.5g / L

Goat anti-human apolipoprotein A1 antibody

10mg / L

Ethylphenyl polyethylene glycol

0.8g / L

magnesium sulfate

0.01g / L

calcium acetate

0.01g / L

[0049] Calibrator:

[0050]ApoA1 antigen standard diluent (20mmol / L phosphate buffer, 2g / L sodium chloride, 0.1g / L sodium azide, 0.4g / L dextran, 1g / L trehalose, 1g / L sucrose, 2g / L bovine serum albumin) was dissolved, tested with a commercially available control reagent and adjusted to 120mg / L, and stored in -20°C in aliquots. Take it out before use, and dilute it into different concentrations of ApoA1 standard with standard diluent (ApoA1 antigen concentration: 0mg / L, 10mg / L, 20mg / L, 50mg / L, 70mg / L). Then use a 0.65 μm filter membrane to filter and sterilize, and store a...

Embodiment 2

[0051] Example 2 Apolipoprotein A1 detection kit

[0052] Reagent R1:

[0053] Phosphate buffer

50mmol / L

potassium chloride

6g / L

phenol

0.7g / L

polyethylene glycol 6000

25g / L

Ethylphenyl polyethylene glycol

51g / L

[0054] Reagent R2:

[0055]

[0056]

[0057] Calibrator:

[0058] ApoA1 antigen standard diluent (50mmol / L glycine buffer, 15g / L sodium chloride, 0.1g / L sodium azide, 50g / L dextran, 25g / L trehalose, 30g / L sucrose, 20g / L L bovine serum albumin) was dissolved, tested with a commercially available control reagent and adjusted to 100 mg / L, and stored in -20°C in aliquots. Take it out before use, and dilute it into different concentrations of ApoA1 standard with standard diluent (ApoA1 antigen concentration: 0mg / L, 10mg / L, 30mg / L, 50mg / L, 70mg / L). Then use a 0.65 μm filter membrane to filter and sterilize, and store at 2-8°C.

Embodiment 3

[0059] Example 3 Apolipoprotein A1 Detection Kit

[0060] Reagent R1:

[0061] acetate buffer

90mmol / L

potassium chloride

20g / L

Sodium azide

0.8g / L

polyethylene glycol 6000

50g / L

Ethylphenyl polyethylene glycol

5g / L

[0062] Reagent R2:

[0063] acetate buffer

90mmol / L

potassium chloride

20g / L

Sodium azide

0.8g / L

Goat anti-human apolipoprotein A1 antibody

300mg / L

Ethylphenyl polyethylene glycol

4g / L

magnesium sulfate

0.05g / L

calcium acetate

0.04g / L

[0064] Calibrator:

[0065] ApoA1 antigen standard diluent (70mmol / L TRIS buffer, 20g / L sodium chloride, 1g / L sodium azide, 70g / L dextran, 60g / L trehalose, 60g / L sucrose, 70g / L bovine serum albumin) was dissolved, tested with a commercially available control reagent and adjusted to 200mg / L, and stored in -20°C. Take it out before use, and dilute it into different concentrations of Ap...

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PUM

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Abstract

The invention provides an apolipoprotein A1 detection kit. The apolipoprotein A1 detection kit comprises a reagent R1 and a reagent R2, wherein the reagent R1 comprises 0.8 to 51g / L of octylphenoxypolyethoxyethanol; the reagent R2 comprises 0.8 to 51g / L of octylphenoxypolyethoxyethanol, 0.01 to 15.5g / L magnesium salt, 0.01 to 20g / L of lime acetate, and an antibody. The kit is good in antibody performance and repeatability, and accurate in reagent detection result, and can meet the use requirement.

Description

technical field [0001] The invention relates to the field of in vitro diagnosis of medical immunity, in particular to an apolipoprotein A1 detection kit and a detection method. Background technique [0002] Turbidimetry has been widely used in clinical testing. Now the most common application is immunoturbidimetry. [0003] Most of the early immunoassay techniques analyzed the presence and content of specific proteins in the sample to be tested by observing the formation of precipitates, the occurrence of agglutination and hemolysis, and measuring the light scattering caused by aggregates, such as immunodiffusion, Immunoelectrophoresis, direct and brief hemagglutination, passive hemagglutination, complement fixation test, etc. These detection methods are low in cost, easy to judge the results, easy to master technically, and can be widely used to detect various types of clinical samples. However, the above method tends to be eliminated due to its cumbersome operation, time...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N21/82G01N21/31
CPCG01N21/31G01N21/82G01N33/68G01N2021/825
Inventor 耿英利罗湘宇甘萍萍黎明龙腾镶
Owner SICHUAN MACCURA BIOTECH CO LTD
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