Lactobacillus plantarum with dual blood glucose-reducing target and application thereof

A Lactobacillus plantarum, hypoglycemic technology, applied in the directions of Lactobacillus, application, bacteria used in food preparation, etc., can solve the problem of little research, and achieve the effects of delaying blood sugar, increasing intake, and improving market prospects

Active Publication Date: 2017-12-15
杨凌集羊农牧发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Numerous studies have shown that probiotics have the potential to reduce the incidence of diabetes, but there is still little research on probiotics as a therapeutic agent for diabetes
In particular, reports on probiotics with dual-target inhibitory capabilities are even rarer

Method used

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  • Lactobacillus plantarum with dual blood glucose-reducing target and application thereof
  • Lactobacillus plantarum with dual blood glucose-reducing target and application thereof
  • Lactobacillus plantarum with dual blood glucose-reducing target and application thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0073] 2. Sample preparation

[0074] (1) Preparation of cell metabolite CFS (cell-free excretory supernatant)

[0075] After the probiotics were statically cultured in MRS medium at 37°C for 15-20h, they were centrifuged at 12000r / min at 4°C for 12-20min to collect the bacteria. The collected cells were washed 2-4 times with phosphate-buffered saline (PBS) with a sterile concentration of 0.1M and a pH value of 6.8, and then resuspended in PBS to adjust the concentration of the cells to 1×10 9 cfu / mL, mix well, incubate at 35-40°C for 10-15h, centrifuge at 12000r / min at 4°C for 12-20min, collect the supernatant, and filter it with a 0.2-0.3μm aqueous microfiltration membrane to obtain CFS, -80 Store at ℃.

[0076] Among them, through a large number of experiments, it was found that after the selected probiotics were cultured for 18 hours, they were centrifuged at 12,000 r / min at 4°C for 15 minutes to collect the bacteria. The collected bacteria were washed 3 times with phos...

Embodiment 1

[0233] Inoculating the Lactobacillus plantarum into cow's milk, goat's milk or camel's milk for fermentation, the fermentation steps are as follows:

[0234] Before inoculation, heat cow’s milk, goat’s milk or camel’s milk to 75°C and keep it for 10s for disinfection. After the cow's milk, goat's milk or camel's milk is cooled to about 40°C, add the Lactobacillus plantarum and stir evenly until foaming. Carry out sealed fermentation at a temperature of about 40° C. for 8-10 hours to obtain fermented cow's milk, fermented goat's milk or fermented camel's milk suitable for diabetics.

Embodiment 2

[0236] The bacteria powder is put into soybean milk or peanut milk for fermentation, and the fermentation steps are as follows:

[0237] Before inoculation, heat soybean milk or peanut milk to 65°C and keep it for 30 minutes for disinfection. After the soy milk or peanut milk is cooled to about 40°C, add the Lactobacillus plantarum and stir evenly until foaming. Carry out sealed fermentation at a temperature of about 40° C. for 8-10 hours to obtain fermented soybean milk or fermented peanut milk suitable for diabetics.

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Abstract

The invention discloses a lactobacillus plantarum with dual blood glucose-reducing target and an application thereof. The lactobacillus plantarum ST-2 with dual blood glucose-reducing target and higher restraining activity is acquired by screening in the manners of in vitro inhibitory activity, probiotic property determination and principal component analysis; the inhibition ratio of the CFS extract thereof for alpha-glucosaccharase activity is 13.44%; the inhibition ratio for dipeptidyl peptidase-IV activity is 21.525%. An experiment proves that ST-2 has a certain inhibitory activity for differently sourced alpha-glucosaccharase and dipeptidyl peptidase-IV; the carbonylated hemoglobin value of type-II diabetic rat is obviously reduced, so that ST-2 has ultrahigh application value for delaying the carbohydrate absorption of the diabetic after the meal and reducing postprandial blood sugar increasing; ST-2 is prepared into freeze-drying bacteria powder in high viable count; the viable count is as high as 89% of that before freeze-drying; besides, ST-2 has an excellent fermenting effect; the application of ST-2 in producing dairy products or being added as a food ingredient into functional food has excellent preventing and intervening effects for diabetes and obesity.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a lactobacillus plantarum with dual hypoglycemic targets and application thereof. Background technique [0002] Diabetes mellitus is a metabolic disorder caused by insufficient insulin secretion (type I) or insulin resistance (type II) and is characterized by chronic hyperglycemia. At present, diabetes has become a major disease, which has caused great damage to human life and health. According to the data of the World Health Organization, by 2017, the prevalence of diabetes in China has ranked first in the world, with about 110 million people, accounting for about one-tenth of the total number of Chinese adults. According to the current trend, by 2040, there will be more than 150 million patients in my country, and the death rate from diabetes will be greater than the sum of the death rates from AIDS, tuberculosis and malaria. Therefore, drugs and functional foods for diabetes hav...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12Q1/37C12Q1/40C12Q1/04A23L33/135C12R1/25
CPCA23L33/135C12Q1/04C12Q1/37C12Q1/40A23V2002/00C12R2001/25C12N1/205A23V2400/169A23V2200/328
Inventor 刘鹭吕加平王芬张书文逄晓阳芦晶
Owner 杨凌集羊农牧发展有限公司
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