Lactobacillus plantarum with dual blood glucose-reducing target and application thereof
A Lactobacillus plantarum, hypoglycemic technology, applied in the directions of Lactobacillus, application, bacteria used in food preparation, etc., can solve the problem of little research, and achieve the effects of delaying blood sugar, increasing intake, and improving market prospects
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[0073] 2. Sample preparation
[0074] (1) Preparation of cell metabolite CFS (cell-free excretory supernatant)
[0075] After the probiotics were statically cultured in MRS medium at 37°C for 15-20h, they were centrifuged at 12000r / min at 4°C for 12-20min to collect the bacteria. The collected cells were washed 2-4 times with phosphate-buffered saline (PBS) with a sterile concentration of 0.1M and a pH value of 6.8, and then resuspended in PBS to adjust the concentration of the cells to 1×10 9 cfu / mL, mix well, incubate at 35-40°C for 10-15h, centrifuge at 12000r / min at 4°C for 12-20min, collect the supernatant, and filter it with a 0.2-0.3μm aqueous microfiltration membrane to obtain CFS, -80 Store at ℃.
[0076] Among them, through a large number of experiments, it was found that after the selected probiotics were cultured for 18 hours, they were centrifuged at 12,000 r / min at 4°C for 15 minutes to collect the bacteria. The collected bacteria were washed 3 times with phos...
Embodiment 1
[0233] Inoculating the Lactobacillus plantarum into cow's milk, goat's milk or camel's milk for fermentation, the fermentation steps are as follows:
[0234] Before inoculation, heat cow’s milk, goat’s milk or camel’s milk to 75°C and keep it for 10s for disinfection. After the cow's milk, goat's milk or camel's milk is cooled to about 40°C, add the Lactobacillus plantarum and stir evenly until foaming. Carry out sealed fermentation at a temperature of about 40° C. for 8-10 hours to obtain fermented cow's milk, fermented goat's milk or fermented camel's milk suitable for diabetics.
Embodiment 2
[0236] The bacteria powder is put into soybean milk or peanut milk for fermentation, and the fermentation steps are as follows:
[0237] Before inoculation, heat soybean milk or peanut milk to 65°C and keep it for 30 minutes for disinfection. After the soy milk or peanut milk is cooled to about 40°C, add the Lactobacillus plantarum and stir evenly until foaming. Carry out sealed fermentation at a temperature of about 40° C. for 8-10 hours to obtain fermented soybean milk or fermented peanut milk suitable for diabetics.
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