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A kind of lotus pod immobilized microorganism and its preparation method and application

A microorganism and lotus plant technology, applied in the field of materials and the environment, can solve the problems of pollution, cadmium and chromium pollution, waste of resources and the environment, etc., and achieve the effects of simple process, resource saving and pollution avoidance.

Active Publication Date: 2019-12-24
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there have been research reports on the extraction of procyanidins (Li Jiaqiao et al., 2016) and the production of crispy biscuits (Lian Chengjie et al., 2006), but in fact, with the expansion of the lotus seed industry in recent years, the main by-product lotus pods have been Random disposal and incineration cause waste of resources and environmental pollution, and resource utilization is urgently needed to avoid environmental pollution
[0003] In addition, due to the intensification of human activities, a large amount of waste water, waste residue and waste gas containing heavy metals such as chromium and cadmium have been produced, resulting in the pollution of heavy metals such as cadmium and chromium in a large number of water bodies and soil environments (Viti et al., 2013)

Method used

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  • A kind of lotus pod immobilized microorganism and its preparation method and application
  • A kind of lotus pod immobilized microorganism and its preparation method and application
  • A kind of lotus pod immobilized microorganism and its preparation method and application

Examples

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Effect test

Embodiment 1

[0031] A method for preparing microorganisms immobilized in lotus houses includes the following steps:

[0032] (1) Preparation of the lotus house: Collect the lotus house in an oven at 55°C, dry it to a constant weight, then pulverize with a grinder, pass through a 60-mesh sieve, and obtain powder for use;

[0033] (2) The lotus house contains microorganisms: a. Inoculate the WH16-1 bacterial solution with a volume fraction of 1% in the logarithmic growth phase in 100 mL of LB medium, and place it in a shaker at 37°C and 160 r / min. After 12h, centrifuge, discard the supernatant, and add 25mL of fresh culture solution; b. Add 0.5g of lotus powder from step (1) and 25g of sodium alginate solution with a mass fraction of 4% to the liquid obtained in step a. Stir to mix thoroughly; c. Pour the mixture obtained in step b into 100g of a calcium chloride solution with a mass concentration of 2%, let it stand for 24 hours and then pour out the liquid, wash the particles with saline, and f...

Embodiment 2

[0036] A method for preparing microorganisms immobilized in lotus houses includes the following steps:

[0037] (1) Preparation of the lotus house: Collect the lotus house in an oven at 55°C, dry it to a constant weight, then pulverize with a grinder, pass through a 60-mesh sieve, and obtain powder for use;

[0038] (2) The lotus house contains microorganisms: a. Inoculate the WH16-1 bacterial solution with a mass fraction of 1% in the logarithmic growth phase into 100 mL of LB medium, add 0.5 g of the lotus house and place it at 37°C, 160r / Cultivate in a shaker for 12 hours for 12 hours; then centrifuge and vacuum dry to obtain the lotus house with immobilized microorganism particles.

[0039] Take the above-mentioned lotus room with microorganisms immobilized in 100mL medium containing 500mg / L Cr(VI) (potassium dichromate), take samples after a certain period of time, filter the samples by centrifugation, and use diphenylcarbazide (DPC) spectrophotometry Method to measure the rem...

Embodiment 3

[0041] The test of the storage stability of the lotus leaf sodium alginate immobilized microorganisms:

[0042] The preparation of lotus palm sodium alginate immobilized microorganism is the same as in Example 1.

[0043] The same number of bacteria is 10 10 Cfu / mL free bacteria powder and lotus alginate sodium alginate immobilized microbial particles were stored in a dry environment at room temperature for 3 months, and then 1.6 g lotus alginate sodium alginate immobilized microbes and free bacteria powder containing the same number of bacteria were taken for 3 months. In 100mL of LB medium, place it in a shaker at 37℃ and 160r / min for 12h, then measure the number of bacteria in the solution by the dilution coating method, the count of free bacteria powder and microbial particles immobilized by lotus sodium alginate Respectively: 0 and 4×10 5 cfu / mL.

[0044] Take the lotus leaf sodium alginate that has been dried and stored for a certain period of time in 100 mL of medium, add pot...

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Abstract

The invention relates to a preparation method of a lotus seed pod immobilized microorganism. The preparation method comprises the following concrete steps of (1) lotus seed pod preparation; (2) lotus seed pod immobilized microorganism obtaining: a, inoculating a WH16-1 bacterium solution in the logarithmic growth period into an LB culture medium according to the standard that the volume fraction is 1 percent; putting the culture medium into a shaking table to be cultured for 12h; then, performing centrifugation; removing supernate; next, adding 25mL of fresh culture liquid; b, adding lotus seed pods and sodium alginate into the bacterium solution; performing stirring so that the mixture is fully mixed; c, injecting mixed liquid obtained in the step b into a certain mass concentration of a calcium chloride solution; after static curing is performed for 6 to 24h, pouring out liquid; cleanly cleaning particulate matters; performing vacuum freeze drying. The preparation method has the advantages that the lotus seed pods are creatively used as an immobilized material; the reutilization of wastes is realized; the resources are greatly saved; moreover, the prepared lotus seed pod immobilized microorganism has excellent performance on heavy metal; the removal rate on 500mg / L Cr(VI) and 50mg / L Cd(II) within 4 days is respectively 95.32 percent and 80.33 percent; good practical values and application prospects are realized.

Description

Technical field [0001] The invention belongs to the technical field of materials and environment, and specifically relates to a lotus house immobilized microorganisms and a preparation method and application thereof. Background technique [0002] The lotus house is the mature receptacle of lotus. It is mainly composed of polyphenols, alkaloids, proteins, sugars, fats, cellulose, proanthocyanidins, vitamins and other chemical substances. It has antioxidant capacity, hemostasis, removes blood stasis, and drains dampness. At present, there have been research reports using it to extract proanthocyanidins (Li Jiaqiao et al., 2016) and make crisp biscuits (Lian Chengjie et al., 2006), but in fact, with the expansion of the lotus seed industry in recent years, the main by-product lotus house has been Random disposal and incineration cause resource waste and environmental pollution, and it is urgent to use them as resources to avoid environmental pollution. [0003] In addition, due to th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N11/12C12N11/10C12N11/02C12N11/14C02F3/34C02F101/22C02F101/20
CPCC02F3/34C02F2101/20C02F2101/22C12N11/02C12N11/10C12N11/12C12N11/14
Inventor 廖水姣石雄鹰王革娇
Owner HUAZHONG AGRI UNIV
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