Method for extracting collagen from animal sebum

A collagen and sebum technology, applied in the biological field, can solve the problems of protein denaturation, low collagen quality, complicated extraction process, etc., and achieve the effect of improved purity and better quality

Inactive Publication Date: 2017-12-15
HEFEI FENGJIE BIOLOGICAL TECH CO LTD
View PDF15 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Extraction methods include acid method, alkali method, enzymatic method, etc., but due to various deficiencies, acid method is rarely used alone. It is generally combined with enzymatic method, and the extraction process is complicated. Alkaline method is easy to cause protein denaturation and is not easy to control. low quality collagen

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0024] The following clearly and completely describes the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only some of the embodiments of the present invention, but not all of them. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0025] In an embodiment of the present invention, a method for extracting collagen from animal sebum comprises the following steps:

[0026] S1, pre-processing:

[0027] S11, weighing 50 parts by weight of animal skin tissue (preferably pig skin or cowhide), degreasing it with a chloroform / ethanol mixed solution, removing subcutaneous fat, removing hair and finely chopping;

[0028] S12, washing the small tissue pieces with ethanol and distilled water for several times;

[0029] S13, put the washed tissue into a blender and stir i...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a method for extracting collagen from animal sebum. The method is based on an acid method for extracting collagen, is improved on the basis of the acid method, uses glacial acetic acid as a carrier to be combined with pepsin as a catalyst so as to avoid problems of generation of odor during use of citric acid and low extraction efficiency of acetic acid, and uses a method of dialysis for separation and purification so as to greatly improve the purity of an obtained collagen solution and achieve better quality.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for extracting collagen from animal sebum. Background technique [0002] Collagen is a biopolymer, the main component of animal connective tissue, and the most abundant and widely distributed functional protein in mammals, accounting for 25% to 30% of the total protein, and even as high as 80% in some organisms . Collagen has been widely used in food, medicine, tissue engineering, cosmetics and other fields due to its good biocompatibility, biodegradability and biological activity. Collagen is extracted from animal skin. In addition to collagen, the skin also contains proteoglycans such as hyaluronic acid and chondroitin sulfate. They contain a large number of polar groups, which are moisturizing factors, and can prevent tyrosine in the skin from converting into melanin. Therefore, collagen has natural moisturizing, whitening, anti-wrinkle, and freckle-removing effects, ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/36C07K1/30C07K1/34
CPCC07K1/303C07K1/34C07K1/36C07K14/78C12P21/06
Inventor 王亚
Owner HEFEI FENGJIE BIOLOGICAL TECH CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap