Synchronous saccharification and fermentation hydrogen production reactor equipped with enzyme recycling and its experimental method

Abstract

A synchronous saccharification and fermentation hydrogen production reactor equipped with enzyme recycling, including an enzymatic hydrolysis unit, a hydrogen production unit, an enzyme desorption unit and an enzyme adsorption unit; the enzymatic hydrolysis unit is located inside the hydrogen production unit; the enzymatic hydrolysis unit is connected to the hydrogen production unit, The lower end of the enzymolysis unit is connected to the feed inlet at the upper end of the enzyme desorption unit, the bottom of the hydrogen production unit is connected to the top of the enzyme adsorption unit through the first support pipe, and the bottom outlet of the enzyme desorption unit is connected to the top of the enzyme adsorption unit through the second support pipe. The top of the enzyme adsorption unit is connected. The invention also discloses an experimental method of a synchronous saccharification and fermentation hydrogen production reactor equipped with enzyme recycling. The present invention recycles the cellulase after the hydrogen production process is over, recycles the cellulase after the enzymolysis reaction, effectively utilizes the residual activity of the cellulase, and improves the sugar yield of the unit enzyme. Further reducing the amount of cellulase can greatly reduce the process cost and promote the industrial production of straw biomass energy conversion.

Description

technical field [0001] The invention belongs to the technical field of rural energy in agricultural engineering, and in particular relates to a synchronous saccharification and fermentation hydrogen production reactor equipped with enzyme recycling and an experimental method thereof. Background technique [0002] The energy shortage and environmental pollution caused by excessive use of fossil energy make it urgent to produce and use clean energy. Hydrogen energy has a high combustion density, clean and pollution-free, and is considered an attractive alternative energy source. Among various hydrogen production methods, biological hydrogen production using microorganisms to produce renewable energy at normal temperature and pressure has become a research hotspot at home and abroad. [0003] Biological hydrogen production mainly includes hydrogen production by photolysis of water, hydrogen production by photofermentation and hydrogen production by dark fermentation. Among the...

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Problems solved by technology

Because if the cellulosic biomass and photosynthetic bacteria are placed in the same reactor, the cellulosic biomass will block the light, which is not conducive to the bacteria receiving light energy, which in turn affects the photosynthesis of photosynthetic bacteria, resulting in low hydrogen production efficiency

[0005] Cellulase plays an indispensable role in the process of biological hydrogen production. It can convert cellulose into fermentable sugar for bacterial fermentation, but due to its high cost, it increases the cost of hydrogen production, which is an obstacle One of the main reasons for the industrial operation of biological hydrogen production

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