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Nanometer enzyme bleeding stopping agent with functions of bleeding stopping and sterilization, and applications thereof

The technology of a hemostatic agent and nano-enzyme is applied in the medical application field of hemostatic antibacterial agent and nano material, which can solve the problems of long time-consuming hemostasis, adhesion, no antibacterial effect, etc., and achieves the effect of simple preparation method and broad-spectrum sterilization.

Inactive Publication Date: 2017-12-22
INSITUTE OF BIOPHYSICS CHINESE ACADEMY OF SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These materials have the following problems: (1) It takes a long time to stop bleeding, and the effect on severe bleeding symptoms is poor; (2) It is easy to stick to the wound, and it is not easy to change the dressing; (3) It does not have antibacterial ability [3]
At present, there are few emergency hemostasis products for severe bleeding at home and abroad. The main products are rapid hemostasis products developed by relying on the super water absorption capacity of zeolite molecular sieves. However, these products will emit a lot of heat when absorbing water. thermal burns [4]
At the same time, such products also do not have antibacterial effects

Method used

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  • Nanometer enzyme bleeding stopping agent with functions of bleeding stopping and sterilization, and applications thereof
  • Nanometer enzyme bleeding stopping agent with functions of bleeding stopping and sterilization, and applications thereof
  • Nanometer enzyme bleeding stopping agent with functions of bleeding stopping and sterilization, and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] The Fe of embodiment 1 polyacrylic acid (PAA), chitosan modification 3 o 4 Synthesis and Characterization of Nanozyme Particles

[0040] In this example, PAA-modified Fc 3 o 4 Nanozyme particles were synthesized using a hydrothermal method. Specifically as follows: 3g FeCl 3 Add 80mL of ethylene glycol and mix well. After fully dissolving, add 6g of anhydrous sodium acetate and 0.6g of PAA, mix thoroughly for 30 minutes, then put the sample into the reaction kettle and react at 200°C for 14 hours. After cooling, magnetically separate, wash with ethanol, dry at 60°C, and store in separate packages. Fe synthesized by this method 3 o 4 The surface of the nanozyme is modified with carboxyl groups.

[0041] In this example, chitosan-modified Fc 3 o 4 The synthesis method of nanozyme particles is also a hydrothermal method. The details are as follows: 3gFeCl 3Add 80mL of ethylene glycol and mix well, after fully dissolving, add 6g of anhydrous sodium acetate, 1g o...

Embodiment 2

[0042] Example 2Fe 3 o 4 Glucose Oxidase Modification of Nanozyme Particles

[0043] In this example, glucose oxidase was modified to Fc 3 o 4 The nanozyme surface adopts the NHS-EDC method. The details are as follows: 5 mg of EDC and NHS were dissolved in 1 mL of deionized water and vortexed. Then, 5 mg of the PAA-modified Fe synthesized above 3 o 4 Nanozymes were added to the solution system and incubated at room temperature for 30 minutes. carboxyl-activated Fe 3 o 4 Nanozyme particles were washed twice with deionized water by magnetic enrichment, and then 0.1 mg / mL glucose oxidase was added. The reaction buffer was 50 mM sodium acetate solution at pH 6. The reaction system was vortexed and incubated overnight at 4°C. Finally, glucose oxidase-modified Fe 3 o 4 Nanozyme particles are washed twice with PBS by magnetic enrichment method, and finally stored in PBS buffer system.

[0044] Fe modified by glucose oxidase 3 o 4 Nanozyme Particles and Chitosan Modifi...

Embodiment 3

[0045] Embodiment 3 The Fc modified by glucose oxidase 3 o 4 Blood coagulation effect of nanozyme particles and its comparison with chitosan-modified nanozymes

[0046] Chitosan has now been widely used as a hemostatic material. In this example, in order to quantitatively compare the Fc modified by glucose oxidase 3 o 4 The coagulation ability of the nanozyme, we choose in the synthesis process, that is, Fe with chitosan modification 3 o 4 nanozyme as a control, and to compare whether glucose oxidase modification affects Fe 3 o 4 Coagulation ability of nanozymes, selected without any modification of Fe 3 o 4 Nanozyme was used as a positive control. In this example, the blood samples are from experimental mice C57BL / 6. The details are as follows: freshly collected mouse blood was anticoagulated with citrate anticoagulant. The principle of citric acid anticoagulation is mainly to chelate Ca in the blood 2+ Ions, because the participation of calcium ions is required i...

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Abstract

The present invention discloses a nanometer enzyme bleeding stopping agent with functions of bleeding stopping and sterilization, and applications thereof. According to the present invention, the nanometer enzyme particles can cause the aggregation of blood platelets and red blood cells in wound blood so as to produce a blood clotting effect while the catalyst modification on the surface of the nanometer enzyme can catalyze the components in blood to produce peroxides such as H2O2, such that the killing of pathogenic bacteria in the wound and the wound infection prevention can be achieved based on the principle; the large-batch and low-cost production of the double-function nanometer enzyme bleeding stopping agent can be achieved; and the nanometer enzyme bleeding stopping agent can be used as the novel antibacterial bleeding stopping agent, has great social and economic benefits, and further has good application prospect.

Description

technical field [0001] The invention belongs to the cross field of nano biomedicine, nano technology and biomedical equipment. In particular, the invention pertains to medical applications of nanomaterials. Specifically, a hemostatic antibacterial agent is provided, which is applied to hemostatic antibacterial treatment of traumatic wounds. technical background [0002] Blood loss and wound infection are the main causes of heavy casualties in emergencies, accidents and wars. Blood loss will directly cause coma and shock of the wounded, and is one of the most common causes of death after mechanical injury to the human body [1] . Wound infection is one of the most serious complications of traumatic wounds such as knife wounds, contusions, and firearm injuries. Wound infection will cause difficulty in wound healing and repair, or even irreparable, or even amputation. The timely hemostasis and antibacterial of the wound is a problem that needs to be solved urgently in the f...

Claims

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Application Information

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IPC IPC(8): A61K33/34A61K33/24A61K33/26A61K33/32A61L15/44A61P31/04A61P31/10A61P7/04
CPCA61K33/24A61K33/26A61K33/32A61K33/34A61L15/44A61L2300/102A61L2300/404A61L2300/418
Inventor 阎锡蕴范克龙高利增段德民张德玺桑建彬
Owner INSITUTE OF BIOPHYSICS CHINESE ACADEMY OF SCIENCES
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