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Freeze-dried dental bone powder material and preparation method thereof

A dental bone and freeze-drying technology, applied in medical science, tissue regeneration, prostheses, etc., can solve problems such as insufficient bone tissue preparation methods, and achieve good biocompatibility

Active Publication Date: 2017-12-22
深圳市光明创博生物制品发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In view of the above deficiencies in the prior art, the object of the present invention is to provide a freeze-dried dental bone powder material and a preparation method thereof, aiming at solving the problem of deficiencies in the bone tissue preparation methods in the prior art

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] Step (1), weigh fresh permanent teeth (human teeth), wash them three times, filter out the water, add 2mol / L disinfectant (peracetic acid), soak for 4 hours at room temperature, filter out the solution, soak in purified water 2 hours, repeated 5 times, that is, repeated filtration and soaking 5 times;

[0066] Step (2), adding the permanent teeth in step (1) to 3mol / L calcium bicarbonate solution for filter sterilization, that is, soaking in the calcium bicarbonate solution, stirring during the soaking process, and soaking for 4 hours in total; then adding In 7mol / L reducing agent zinc chloride, continue to stir for 4 hours, then soak for 24 hours; filter the solution, then inject purified water and rinse 5 times, each time for 20min, to obtain material A;

[0067] Step (3), adding the above-mentioned material A to a mixed solution of xylene and diethane (the mass ratio of the two can be 1:1, and the composition of the mixed solution in subsequent examples can also be 1...

Embodiment 2

[0074] Step (1), weigh fresh permanent teeth (pig teeth), wash three times, filter out the water, add 0.5mol / L disinfectant (sodium hypochlorite), soak for 10 hours at room temperature, filter out the solution, soak in purified water for 1 hour , repeated 8 times, that is, repeated filtration and soaking 8 times;

[0075] Step (2), adding the permanent teeth in step (1) to 5mol / L calcium oxalate solution for filter sterilization, that is, soaking in the calcium oxalate solution, stirring during the soaking process, and soaking for 0.1 hour; adding 2mol / L In L reducing agent sodium borohydride, continue to stir for 10 hours, then soak for 1 hour; filter off the solution, then inject purified water to rinse once, each time for 10 hours, to obtain material A;

[0076] Step (3), adding the above-mentioned material A into the mixture of acetone and cyclohexanone to filter and sterilize, that is, adding it to the above-mentioned mixture for soaking, stirring during the soaking proce...

Embodiment 3

[0083] Step (1), weigh fresh permanent teeth (sheep teeth), wash them three times, filter out the water, add 10mol / L disinfectant (peracetic acid), soak at room temperature for 0.5 hours, filter out the solution, soak in purified water 10 hours, repeated 3 times, that is, repeated filtration and soaking 3 times;

[0084] Step (2), add the permanent teeth in step (1) to 0.1mol / L calcium dihydrogen phosphate solution for filter sterilization, that is, soak in the calcium dihydrogen phosphate solution, stir during the soaking process, and soak for 10 hours in total Add 15mol / L reducing agent lithium chloride, continue to stir for 0.1 hour, then soak for 48 hours; filter the solution, then inject purified water and rinse for 10 times, each time for 10min, to obtain material A;

[0085] Step (3), adding the above-mentioned material A into the mixture of chloroform and carbon tetrachloride to filter and sterilize, that is, adding to the above-mentioned mixture for soaking, stirring ...

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Abstract

The invention discloses a freeze-dried dental bone powder material and a preparation method thereof. The method includes the steps of: weighing permanent teeth, and conducting cleaning and disinfection on the permanent teeth; performing degerming treatment on the permanent teeth, then adding a reducing agent, performing stirring for 0.1-010h, and conducting soaking for 1-48h; filtering out the solution, and conducting rinsing to obtain a material A; subjecting the material A to degerming treatment, then performing filtering to obtain a solid, and conducting rinsing to obtain a material B; carrying out fragmentation treatment on the material B to obtain a material C; adding the material C into an acid solution, performing stirring, and then conducting soaking for 1-48h; filtering out the solution, and performing rinsing to obtain a material D; soaking the material D in an alcohol solution; and subjecting the prepared material D to freeze-drying, thus obtaining the freeze-dried dental bone powder material. The product provided by the invention has good biocompatibility, is beneficial to transportation and vascularization of nutrients and metabolites, and is in favor of induction of osteogenic factors and bone tissue construction, and synchronous growth with autologous bone.

Description

technical field [0001] The invention relates to the field of medical materials, in particular to a freeze-dried dental bone powder material and a preparation method thereof. Background technique [0002] Urist in the United States found that the decalcified bone matrix can induce osteogenesis, and then proposed the theory of osteoinductive repair containing bone morphogenetic protein (BMP). Since then, a series of osteogenic materials derived from organisms have been developed at home and abroad, including autologous bone materials and allogeneic bone. Autologous bone has many advantages such as no rejection, good osteoinductive activity, etc., and is the gold standard for bone graft materials, but it also has many disadvantages—it will cause secondary trauma to the patient, lead to functional loss of the bone donor site, and the patient’s physical fitness and age restricted. The allogeneic bone source is relatively sufficient and convenient to use, but it is easy to cause...

Claims

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Application Information

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IPC IPC(8): A61L27/36A61L27/56
CPCA61L27/3608A61L27/365A61L27/3687A61L27/3691A61L27/56A61L2430/02A61L2430/40
Inventor 毕晓寰张明
Owner 深圳市光明创博生物制品发展有限公司
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