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Livestock adenovirus strain, vaccine composition and application thereof

A technology of vaccine composition and poultry adenovirus, which is applied in the field of biomedicine, can solve the problems that it is difficult for vaccines to provide ideal immune effects, it is difficult to obtain high-titer viruses, and it is difficult to cultivate poultry adenoviruses, so as to achieve good virus proliferation titers, Good immunogenicity, good effect of immunogenicity

Active Publication Date: 2017-12-29
PU LIKE BIO ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Vaccine immunization can effectively prevent the disease. However, in the existing technology, because avian adenovirus is not easy to cultivate, it is difficult to obtain high-titer virus, especially the difference between different isolates, which makes it difficult for the prepared vaccine to provide the ideal immune effect.

Method used

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  • Livestock adenovirus strain, vaccine composition and application thereof
  • Livestock adenovirus strain, vaccine composition and application thereof
  • Livestock adenovirus strain, vaccine composition and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment approach

[0039] As an embodiment of the present invention, in the vaccine composition of the present invention, the vaccine composition also includes one or more of the following antigens: chicken Newcastle disease virus antigen, avian influenza virus antigen, infectious bronchitis virus antigen, Chicken Infectious Bursal Virus Antigen, Egg Drop Syndrome Virus Antigen, Avian Reovirus Antigen, Escherichia coli Antigen, Avian Bacteria Paragallily Antigen, Mycoplasma Synovial Bursae, Mycoplasma Gallisepticum Antigen, Pasteurella multocida Antigen , Marek's virus antigen, avian encephalomyelitis virus antigen, or chicken infectious laryngotracheitis virus antigen.

[0040] The vaccine composition of the present invention further comprises other pathogens or antigens and is used in combination to prepare combined vaccines or compound vaccines against various diseases including serotype 4 avian adenovirus infection.

[0041] The term "combination vaccine" is used to refer to a vaccine prepar...

Embodiment 1

[0059] Example 1 Isolation and identification of serotype 4 avian adenovirus FAV-HN strain

[0060] Treatment of diseased chickens sent for inspection by a white-feathered broiler farm in Henan: take part of the liver tissue of the diseased chicken, add the same amount of PBS containing antibiotics for grinding, and centrifuge the grinding liquid to get the supernatant, and inoculate it through the allantoic cavity for 10 days Young SPF chicken embryos were cultured at 37°C. Chicken embryos that died within 24 hours after inoculation were discarded, and the allantoic fluid of chicken embryos that died 24 to 168 hours after inoculation and those that survived 168 hours were harvested.

[0061] Take harvested allantoic fluid for identification:

[0062] (1) Hemagglutination identification: 1% chicken red blood cells were used to detect the hemagglutination of the harvested allantoic fluid samples. The result was negative, indicating that the sample had no hemagglutination acti...

Embodiment 2

[0064] The preparation of embodiment 2 poultry adenovirus (chicken embryo source) inactivated vaccine

[0065] Dilute the seed virus of the avian adenovirus FAV-HN strain 10 times with sterilized normal saline, inoculate 5-7 day-old SPF chicken embryos through the yolk sac, inoculate 0.1ml per embryo, and continue incubation at 37°C. Dead embryos were discarded within 24 hours after inoculation, and the allantoic fluid of 24-144-hour dead embryos and 144-hour surviving embryos was harvested in time. Take a small sample of the harvested virus liquid for sterility testing, and store it at low temperature for later use. After passing the inspection, the virus liquid was mixed, and a sample was taken to detect the half-infection dose (EID) of chicken embryos. 50 ).

[0066] Embryo median infectious dose (EID) 50 ) Assay method: the harvested poultry adenovirus FAV-HN strain virus liquid is carried out 10 times of serial dilutions with sterilized physiological saline: 10 -2 、10 ...

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Abstract

The invention provides a serum type-4 livestock adenovirus strain FAV-HN. The strain has good immunogenicity. A vaccine composition prepared from an antigen of the strain can rapidly generate antibodies after immune, and chickens can be completely protected by using a low-content vaccine composition. In addition, the antigen has combined action with multiple other antigens, and the immunity effects are not affected by the antigens.

Description

technical field [0001] The invention relates to a poultry adenovirus strain, a vaccine composition prepared from the strain and an application thereof, belonging to the field of biomedicine. Background technique [0002] Hydropericardium syndrome (HPS) is a highly contagious disease of poultry caused by serotype 4 avian adenovirus (FAV-4). Broiler chickens aged 3 to 5 weeks are particularly dangerous, and the mortality rate suddenly rises to more than 80% in severe cases. The pathogen of the disease - serotype 4 avian adenovirus (FAV-4), is a member of Adenoviridae, avian adenovirus genus, and avian adenovirus C species. FAV-4 is a non-encapsulated icosahedral symmetrical structure with a diameter of 70-90nm. The genome is a linear double-stranded DNA with a length of about 43Kb. Necrotic foci on the liver surface and basophilic inclusion bodies in the nuclei of hepatocytes were common in infected chickens. [0003] Vaccine immunization can effectively prevent the disease...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N7/00A61K39/235A61K39/39A61P31/14A61P9/00A61P7/10C12R1/93
CPCA61K39/12A61K39/39A61K2039/5252C12N7/00C12N2710/10221C12N2710/10234
Inventor 田克恭刘武杰孙进忠张许科
Owner PU LIKE BIO ENG
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