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System for nucleic acid purification and amplification and gene detection and application method thereof

A gene detection and nucleic acid technology, applied in biochemical equipment and methods, bioreactors/fermenters for specific purposes, biochemical instruments, etc., can solve the problems of large size, long detection time, and complicated operation.

Active Publication Date: 2018-01-05
BEIJING INSTITUTE OF TECHNOLOGYGY
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  • Abstract
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  • Claims
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Problems solved by technology

[0004] In order to overcome the problems of traditional nucleic acid detection equipment such as huge volume, long detection time and complicated operation, the object of the present invention is to provide a system and application method for nucleic acid purification, amplification and gene detection. The system and application method are a Nucleic acid purification-amplification-detection integrated system and application method based on micro-magnet and magnetic control automation system

Method used

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  • System for nucleic acid purification and amplification and gene detection and application method thereof
  • System for nucleic acid purification and amplification and gene detection and application method thereof
  • System for nucleic acid purification and amplification and gene detection and application method thereof

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Embodiment 1

[0050] The hemolysis-related genes in the human body are E2, E23 or E4, and blood transfusion between individuals with different genotypes is very likely to cause symptoms such as hemolysis. In this embodiment, the genotype related to human hemolysis is judged by the system and its application method for nucleic acid purification, amplification and gene detection described in the present invention.

[0051] A system for nucleic acid purification, amplification and gene detection, such as Figure 1~3 The system shown includes a substrate 1, a microfluidic chip, a micromagnet 9, a magnet array control mechanism, a heating component 14, an excitation light generating component 15, an optical sensor 16, a driver 17, a controllable voltage regulator 18, and a system DC power supply. 19 and microcontroller 20.

[0052] Wherein, the base 1 is an aluminum alloy base attached with a flexible magnet.

[0053] The structure of the microfluidic chip used in this embodiment is as follows...

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Abstract

The invention relates to a system for nucleic acid purification and amplification and gene detection and an application method thereof, and belongs to the technical field of apparatus for biological separation, analysis and purification and gene detection. The system comprises a base, a micro fluidic chip, micro magnets, a magnet array control mechanism, a heating element, a laser generating element, an optical sensor, a driver, a controllable voltage stabilizer, a system direct-current power source and a single-chip microcomputer; according to application, nucleic acid purification and amplification and gene detection are conducted by means of the system. According to the system and the application method, the size of a nucleic acid purification-amplification-detection device is reduced,the period of nucleic acid detection is shortened, the detection throughput is increased, the complexity of user operation is lowered, multiple functions of purification, amplification and gene detection can be achieved, and the problems of large size, long detection time and complex operation of conventional nucleic acid detection equipment are solved.

Description

technical field [0001] The invention relates to a system and an application method for nucleic acid purification, amplification and gene detection, and belongs to the technical field of biological separation analysis and purification instruments and gene detection. Background technique [0002] At present, the integrated detection and analysis process for nucleic acid can be widely used in clinical, epidemic prevention, and public security criminal investigation. Traditional nucleic acid purification methods mostly use centrifugation, adsorption column solid-phase extraction and other programs. The centrifugation method requires larger centrifugal equipment, and the operation time is longer and the complexity is higher. The adsorption column solid-phase extraction method requires the participation of many valves and pump structures; and this method adopts the method of liquid circulation washing to achieve purification, resulting in low reagent utilization and other problem...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/38C12M1/00C12Q1/68C12N15/10
Inventor 邓玉林张德雨张永谦刘可夫董一名耿利娜闫天翼
Owner BEIJING INSTITUTE OF TECHNOLOGYGY
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