Insecticide d-phenothrin degradation strain, as well as microbial agent and degradation process thereof

A technology of d-phenothrin and degrading bacteria, applied in the direction of bacteria, chemical instruments and methods, biochemical equipment and methods, etc., can solve the problems of excessive d-phenothrin residues, environmental pollution, etc., and achieve the goal of solving the problem of pesticides The problem of excessive residues and environmental pollution, the effect of good degradation effect

Active Publication Date: 2018-01-05
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved in the present invention is to overcome the defects and deficiencies of the existing d-phenothrin-degrading microbial preparations, and provide a microbial strain and The bacterial agent produced by it and the optimal degradation process conditions
Direct applicati

Method used

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  • Insecticide d-phenothrin degradation strain, as well as microbial agent and degradation process thereof
  • Insecticide d-phenothrin degradation strain, as well as microbial agent and degradation process thereof
  • Insecticide d-phenothrin degradation strain, as well as microbial agent and degradation process thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] Isolation and Identification of Example 1 Bacterial Strain P31

[0056] 1. Isolation and screening of strains

[0057] (1) Sample: Collected from the activated sludge at the sewage discharge outlet and surrounding areas of a chemical plant in Guangxi.

[0058] (2) The separation and screening method adopts the enrichment culture method, as follows:

[0059] Add 100 mL of basic mineral salt medium (MSM) into a 250 mL Erlenmeyer flask and sterilize (121° C. for 20 min). After cooling the culture medium, add d-phenothrin sample mother solution (acetone as solvent) under aseptic conditions, so that the final mass concentration of d-phenothrin is 100mg / L. After 7 days of culture on a shaker at 200r / min, transfer to the second batch of MSM medium containing 200mg / L d-phenothrin according to the inoculum size of 10%. After culturing under the same conditions for 7 days, transfer them to the basal medium containing 400 mg / L d-phenothrin according to 10% inoculum size, and co...

Embodiment 2

[0077] The preparation of embodiment 2 bacterial strain P31 degrading bacteria agent

[0078] 1. The production process of using the above-mentioned bacterial strain P31 to prepare the degrading bacterial agent is as follows:

[0079] Inclined species - shake flask seed solution - seed tank culture - production tank fermentation - product (packaging dosage form is liquid dosage form or solid microbial dosage form).

[0080] 2. Specifically, the method for preparing the degrading bacterial agent is as follows:

[0081] (1) Activate the bacterial classification of bacterial strain P31 on the LB solid plate, inoculate it on the inclined surface of the LB test tube for subsequent use;

[0082] (2) Inoculate the test tube species of bacterial strain P31 in a 1000mL shake flask containing 250mL LB medium (medium formulation: 0.5% yeast extract, 1% peptone, 1% NaCl, pH value 7.2~7.5), 30°C Oscillate at constant temperature to the logarithmic phase to obtain strains;

[0083] (3) T...

Embodiment 3

[0086] The optimal degradation process condition screening optimization of embodiment 3 bacterial strain P31 inoculum

[0087] 1. Using the combination of single factor experiment and response surface method, the characteristics of strain P31 degrading d-phenothrin were determined, and the degradation conditions of the strain were optimized at the same time.

[0088] The P31 bacterial agent was subjected to a single-factor degradation test, and the degradation rate of the P31 bacterial agent was determined by sequentially changing the factors that affect growth and degradation, such as temperature, pH value, inoculum size, oscillation rate, liquid volume, etc., to determine the impact on the P31 bacterial agent. key factor in degradation rate. Use SAS 9.0 statistical software to carry out experimental design (Table 3) according to the Response Surface Method Box-Behnken design principle, to affect the key factor temperature value (X 1 ), pH(X 2 ) and inoculation volume (X 3...

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Abstract

The invention discloses an insecticide d-phenothrin degradation strain, as well as a microbial agent and a degradation process thereof. The strain is Pseudomonas fulva strain P31, and is preserved inChina Centre for Type Culture Collection on May 12th, 2017, and the preservation number is CCTCC NO: M2017256. The strain and the microbial agent thereof have a very good degradation effect on a plurality of pyrethriods pesticides, particularly on d-phenothrin; the degradation rate of the strain and the microbial agent of the strain to 50mg/L d-phenothrin reaches 86.4 percent within 48 hours, andthe 50mg/L d-phenothrin can be completely degraded within 72 hours; the residual amount of the d-phenothrin in a water body or soil can be reduced by 77 percent or above within a short time by directly applying the microbial agent; the microbial agent can be used for repairing environments, such as the water body and the soil which are polluted by the pyrethriods pesticides; the problems that residue of pesticides exceeds a standard and causes environmental pollution in agricultural production are solved; non-toxic pollution-free green agricultural products are produced.

Description

technical field [0001] The invention belongs to the technical field of pesticide pollution treatment. More specifically, it relates to an insecticide d-phenothrin-degrading bacterial strain and its production bacterial agent and optimal degradation process conditions. Background technique [0002] The advent and use of pesticides have brought huge economic and social benefits to humans, especially in protecting crops, preventing pests and weeds, and improving the human living environment. However, the continuous use of pesticides will inevitably cause pollution, cause pesticide residues in food, and cause negative impacts on human health. In recent years, people's living standards have been continuously improved, and the whole society has paid more and more attention to the environment and ecological balance. It is imminent to find new technologies for efficient, economical, safe and stable control of environmental and pesticide residue pollution in agricultural products. ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34B09C1/10C12R1/38C02F101/34
Inventor 陈少华冯彦媚占卉范兴辉叶田张恺扬何梓琪
Owner SOUTH CHINA AGRI UNIV
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