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Applications of 9-hydroxyl-3-isophenoxazone in matrix-assisted laser desorption ionization

A matrix-assisted laser and isophenoxazinone technology is applied in the detection field to achieve the effects of ensuring integrity, avoiding the formation of salt crystals, and reducing background interference

Active Publication Date: 2018-01-05
INST OF CHEM CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] However, particle mass determination using ion trap mass spectrometry still needs further development and improvement

Method used

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  • Applications of 9-hydroxyl-3-isophenoxazone in matrix-assisted laser desorption ionization
  • Applications of 9-hydroxyl-3-isophenoxazone in matrix-assisted laser desorption ionization
  • Applications of 9-hydroxyl-3-isophenoxazone in matrix-assisted laser desorption ionization

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] In this example, polystyrene spheres and red blood cells were used as particles to be analyzed, and the inventors introduced in detail the use of 9-hydroxy-3-isophenoxazinone as a matrix for matrix-assisted laser desorption ionization and ion trap mass spectrometry Analyzing the experimental procedure.

[0038] 1) Disperse 9-hydroxyl-3-isophenoxazinone in a mixed solution of acetonitrile and water with a volume ratio of 1:1, and the mixed solution contains 0.1v% trifluoroacetic acid to prepare 9-hydroxyl- The content of 3-isophenoxazinone is 1 mg / mL suspension, and the suspension is ultrasonically mixed.

[0039] 2) The 3 μm polystyrene spheres were first washed three times in deionized water and collected by centrifugation, and finally dispersed in deionized water to control the concentration at about 2 mg / mL.

[0040] Kunming mouse erythrocytes were first washed 3 times in PBS solution to remove the serum part, and then soaked in PBS containing 0.25% glutaraldehyde f...

Embodiment 2

[0047] In the development process of the previous experimental method, the inventor used polystyrene spheres as the particles to be analyzed, considering that 9-hydroxy-3-isophenoxazinone, anthraquinone 1,5 disulfonic acid sodium salt, nuclear fast red, The six molecules of cresyl violet, rhodamine B, and rose bengal all have strong absorption at 532nm, so they may be used as matrix molecules for visible light MALDI, and the inventors further compared the performance of these six molecules as matrix molecules through experiments. Desorption ionization efficiency.

[0048] The inventors followed the method described in Example 1 to prepare matrix solution, sample preparation and mass analysis of 3 μm polystyrene spheres. The mass spectra obtained by the 6 matrices are as follows Figure 6 As shown, the results show that: anthraquinone 1,5 disulfonic acid sodium salt, cresyl violet, rhodamine B and 9-hydroxy-3-isophenoxazinone can achieve good desorption and ionization of 3 μm ...

Embodiment 3

[0050] Furthermore, in the development process of the previous experimental method, the inventors investigated the change of the desorption ionization efficiency with the concentration of 9-hydroxy-3-isophenoxazinone. During the investigation, polystyrene balls were used as the To analyze the particles, the specific experimental process is as follows:

[0051] 9-Hydroxy-3-isophenoxazinone was formulated as 0.25 mg / mL, 0.5 mg / mL, 1 mg / mL and 2.5 mg / mL respectively according to the method in Example 1. And the analysis was performed on 3 μm polystyrene spheres. The desorption ionization efficiency of 9-hydroxy-3-isophenoxazinone with its concentration was investigated by using the average number of signals in each spectrum as an index. The result is as Figure 7 Shown, wherein, the desorption ionization efficiency of 3 μ m polystyrene spheres represents with the average number of signals in the spectrogram (n=30), and the result shows: when the concentration of 9-hydroxyl-3-is...

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Abstract

The invention provides applications of 9-hydroxyl-3-isophenoxazone in matrix-assisted laser desorption ionization, and method used for measuring the mass of particles to be measured. The acidity of 9-hydroxyl-3-isophenoxazone is weak, no corrosion on particle samples is caused by 9-hydroxyl-3-isophenoxazone, the maximum absorption wavelength is at visible light region, the absorption of most largemolecular compounds at visible light region is weak, so that 9-hydroxyl-3-isophenoxazone is capable of reducing sample excitation and breaking caused by laser effectively. When 9-hydroxyl-3-isophenoxazone is applied in matrix-assisted laser desorption ionization, complete particles can be obtained, and especially high efficiency desorption and ionization of biological particles without hard shells, such as large particles and cells, can be realized. The method used for measuring the mass of particles to be measured is capable of avoiding defects in the prior art that the completeness of particles without hard shells such as cells is difficult to be maintained by organic small molecular matrix. The matrix used in the method is low in background interference, and high in desorption ionization efficiency, and is suitable for mass analysis and measuring of a plurality of inorganic, organic, and biological particles.

Description

technical field [0001] The present invention relates to the detection field, specifically, the present invention relates to the application of 9-hydroxyl-3-isophenoxazinone in matrix-assisted laser desorption ionization, more specifically, the present invention relates to 9-hydroxyl-3-isophenoxazine Use of ketones in matrix-assisted laser desorption ionization and a method for determining the mass of particles to be tested. Background technique [0002] Particles are a widespread form of basic matter, such as viruses, bacteria, cells, etc. in the biological field, dust, aerosols, etc. drug carrier, etc. These particles play important roles in fields such as biomedicine, environment and materials science, so the characterization of these particles is crucial. Particle mass is an important physical parameter for particle characterization, which can comprehensively reflect the volume and density information of particles. In addition, compared with the volume and density, whi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/64
Inventor 熊彩侨聂宗秀王继云黄熹刘会会
Owner INST OF CHEM CHINESE ACAD OF SCI
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