Preservation method of probiotics

Abstract

The invention provides a bacteria preservation method for increasing activity of probiotics, and belongs to the technical field of food microorganism. The bacteria preservation method comprises the following steps: 1, inoculating probiotics bacteria to a proper liquid culture medium, and culturing to a logarithmic growth period under an appropriate condition; 2, properly concentrating a probioticssuspension solution obtained through culturing, or collecting bacteria through a centrifuging method; 3, uniformly mixing the probiotics suspension solution or the collected bacteria with sterilizedmicroalgae powder according to a certain proportion; 4, preculturing a probiotics and algae powder mixture for 2 to 4 hours under an appropriate condition; 5, subpackaging, directly preserving or carrying out vacuum drying under an appropriate condition, and preserving at -40 to -20 DEG C after aseptically packaging. According to the bacteria preservation method provided by the invention, the cellactivity of the probiotics can be better kept, the number of living cells in a preserved object can be remarkably increased, the problems of low preservation survival rate and low activity of bacteria in probiotics food and healthcare product production can be well solved, and a chemical protective agent is avoided in a bacteria preservation process.

Description

technical field [0001] It belongs to the technical field of microorganisms and relates to a preservation method of probiotics. Background technique [0002] Probiotics are microbial supplements that exert beneficial effects on the host by improving the balance of gut microbes. Studies have found that probiotics have great physiological effects in regulating the structure of intestinal flora, enhancing immunity, reducing cholesterol, and eliminating carcinogenic factors. meaning. Moreover, in the face of environmental problems, lifestyle and food hygiene problems that bring hidden dangers to people's health, probiotics have great potential in maintaining health and reducing the risk of disease, which makes probiotics in food and biomedical products. and other fields have been more and more widely used. [0003] At present, domestic probiotic products are mainly fermented yogurt and dairy products, and there are more and more other types of probiotic products. However, in o...

AI Technical Summary

Problems solved by technology

[0007] In order to overcome the deficiencies of low survival rate, low cell viability, and complicated operation steps and preservatives in the existing probiotic seed preservation technology, the present invention provides a preservation method for probiotics, which uses edible microalgae powder as a single preservation method. agent, has high nutritional value, does not add any harmful chemical substances, and can maintain the purity and safety of probiotics to the greatest extent; at the same time, the raw materials of the present invention are easy to obtain, the steps are simple, and the operation is convenient, which avoids expensive maintenance procedures such as programmed cooling devices. This equipment is not only suitable for general laboratory research and development purposes, but also suitable for the preservation of probiotics involved in the production and processing of the food industry

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