Preservation method of probiotics

A preservation method, a technology of probiotics, applied in the field of microorganisms, can solve the problems of low cell viability, low survival rate, complicated operation steps and preservatives, etc., achieve high cell viability, solve the effect of low survival rate, and be easy to promote

Active Publication Date: 2018-01-09
青岛藻蓝生物有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In order to overcome the deficiencies of low survival rate, low cell viability, and complicated operation steps and preservatives in the existing probiotic seed preservation technology, the present invention provides a preservation method for probiotics, which uses edible microalgae powder as a single preservation method. agent, has high nutritional value, does not add any harmful chemical substances, and can maintain the purity and safety of probiotics to the greatest extent; at the same time, the raw materials of the present invention are easy to obtain, the steps are simple, and the operation is convenient, which avoids expensive maintenance procedures such as programmed cooling devices. This equipment is not only suitable for general laboratory research and development purposes, but also suitable for the preservation of probiotics involved in the production and processing of the food industry

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Example 1: Preservation of Lactobacillus acidophilus in kudzu rice powder

[0044] 1.1 Culture of Lactobacillus acidophilus

[0045] Pick a single colony from the Lactobacillus acidophilus preserved on the slant and inoculate it into the MRS liquid medium, place it at 37°C, and let it stand for about 24 hours to obtain a bacterial suspension in the logarithmic growth phase, and adjust the cells of the bacterial suspension. density, making it OD 650nm =0.8, the number of viable bacteria is about 1.0×10 7 cfu / ml.

[0046] 1.2 Mixing of Lactobacillus acidophilus and kudzu rice powder

[0047] Weigh 2 parts of 0.05g Ge Xian Mi powder, spread them on a plate with a diameter of 9 cm, place them on a sterile operating table, sterilize them by ultraviolet radiation for 20 minutes, and then add the prepared bacterial suspension to one of the powders. 0.9 ml of the bacterial suspension was used as the seed preservation test group, the other was added with 0.9 ml of sterile ph...

Embodiment 2

[0054] Example 2: Preservation of Lactobacillus bulgaricus in Spirulina

[0055] 1.1 Culture of Lactobacillus bulgaricus

[0056] Pick a single colony from the slant of Lactobacillus bulgaricus and inoculate it into MRS liquid medium, add 8% tomato juice, 10% wort and 2% whey, adjust the initial pH of the medium to 5.8, and culture it anaerobically at 40°C for 15 hours , the bacterial suspension in logarithmic growth phase is obtained, and the number of viable bacteria in the adjusted bacterial suspension is about 1.0×10 7 cfu / ml.

[0057] 1.2 Mixing of Lactobacillus bulgaricus and Spirulina powder

[0058] Weigh 2 parts of 0.05g spirulina powder, spread them in a plate with a diameter of 9cm, place them on a sterile operating table, sterilize by ultraviolet radiation for 20 minutes, and then add cultured Lactobacillus bulgaricus to one of the powders. 0.9ml of the suspension was used as the seed preservation test group, the other was added with 0.9ml of sterile physiologic...

Embodiment 3

[0065] Example 3: Preservation of Lactobacillus acidophilus and Lactobacillus bulgaricus in kudzu rice powder under dry conditions

[0066] 1.1 Cultivation of probiotics

[0067] According to the methods of Examples 1 and 2, two kinds of probiotic bacteria suspensions in the logarithmic growth phase were obtained by culturing respectively, and the number of viable bacteria in the adjusted bacteria suspension was about 1.0×10 7 cfu / ml.

[0068] 1.2 Mixing and drying of probiotics and kudzu powder

[0069] Weigh 4 parts of each 0.05g Ge Xian Mi powder and spread them in a plate with a diameter of 9 cm, place them on a sterile operating table, sterilize them by ultraviolet radiation for 20 minutes, and then add 2 parts of the algal powder to the algal powder. 0.4ml each of Lactobacillus acidophilus and Lactobacillus bulgaricus was used as the seed preservation test group, and 0.4ml of sterile saline was added to the other two algal powders as the blank control group. Each 0.4m...

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Abstract

The invention provides a bacteria preservation method for increasing activity of probiotics, and belongs to the technical field of food microorganism. The bacteria preservation method comprises the following steps: 1, inoculating probiotics bacteria to a proper liquid culture medium, and culturing to a logarithmic growth period under an appropriate condition; 2, properly concentrating a probioticssuspension solution obtained through culturing, or collecting bacteria through a centrifuging method; 3, uniformly mixing the probiotics suspension solution or the collected bacteria with sterilizedmicroalgae powder according to a certain proportion; 4, preculturing a probiotics and algae powder mixture for 2 to 4 hours under an appropriate condition; 5, subpackaging, directly preserving or carrying out vacuum drying under an appropriate condition, and preserving at -40 to -20 DEG C after aseptically packaging. According to the bacteria preservation method provided by the invention, the cellactivity of the probiotics can be better kept, the number of living cells in a preserved object can be remarkably increased, the problems of low preservation survival rate and low activity of bacteria in probiotics food and healthcare product production can be well solved, and a chemical protective agent is avoided in a bacteria preservation process.

Description

technical field [0001] It belongs to the technical field of microorganisms and relates to a preservation method of probiotics. Background technique [0002] Probiotics are microbial supplements that exert beneficial effects on the host by improving the balance of gut microbes. Studies have found that probiotics have great physiological effects in regulating the structure of intestinal flora, enhancing immunity, reducing cholesterol, and eliminating carcinogenic factors. meaning. Moreover, in the face of environmental problems, lifestyle and food hygiene problems that bring hidden dangers to people's health, probiotics have great potential in maintaining health and reducing the risk of disease, which makes probiotics in food and biomedical products. and other fields have been more and more widely used. [0003] At present, domestic probiotic products are mainly fermented yogurt and dairy products, and there are more and more other types of probiotic products. However, in o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/04C12R1/23C12R1/245C12R1/225C12R1/01C12R1/25
Inventor 陈伟段超刘永梅
Owner 青岛藻蓝生物有限公司
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